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61.
Using the polymerase chain reaction (PCR) and two primers for conserved regions of the small subunit ribosomal RNA (SSU-rRNA) of Microsporidia, a DNA segment about 1,195 base pairs long was amplified from a DNA template prepared from purified spores of the microsporidian species Pleistophora anguillarum. These spores had been isolated from adult eels (Anguilla japonica) with "Beko Disease." A comparison of sequence data from other microsporidian species showed P. anguillarum SSU-rRNA to be most similar to Vavraia oncoperae. When juvenile eels were artificially infected with P. anguillarum, enzyme-linked immunosorbent assay could detect a positive infection only 12 days post-infection. However, when suitable PCR primers were used, a DNA fragment of about 0.8 kb was detected from these juvenile eels after only 3 days post infection. No PCR product was obtained with templates prepared from clinically healthy control animals. 相似文献
62.
Transmission electron microscopy study of a dental gallium alloy 总被引:1,自引:0,他引:1
A. E. Gunnæs A. Olsen H. Herø 《Journal of materials science. Materials in medicine》1996,7(7):447-455
Analytical transmission electron microscopy (TEM) studies of a dental gallium alloy have been carried out. This commercial Ga alloy was made by triturating a Ag-Sn-Cu-rich alloy powder with a liquid Ga alloy containing Ga, In and Sn. Ga alloys are of increasing interest as an alternative to amalgam. The dental material studied in the present work was found to be a composite consisting of remaining, undissolved particles from the Ag-based alloy powder in a matrix of reaction products with the liquid Ga alloy. The phases in the matrix and the remaining Ag-based alloy particles have been identified by electron diffraction, high resolution electron microscopy and energy dispersive X-ray spectroscopy. the following phases were identified: orthorhombic Ag3Sn, cubic -Cu9Ga4, cubic Ag9In4, tetragonal -Sn and hexagonal Ag2Ga. In addition to these well-known phases Ga-rich regions of Cu-Ga were observed consisting of an intergrowth of the tetragonal CuGa2 and one of the cubic -Cu9Ga4 phases. 相似文献
63.
Asbjørn Solheim Sverre Rolseth Egil Skybakmoen Lisbet Støen Åsmund Sterten Trond Støre 《Metallurgical and Materials Transactions B》1996,27(5):739-744
Temperatures for primary crystallization of Na3AlF6 in multicomponent electrolyte systems of interest for the aluminum electrolysis process were determined by thermal analysis. The results are presented as binary and quasibinary diagrams and discussed in view of the literature data. An empirical equation describing liquidus temperatures for primary crystallization of Na3AlF6 was derived: $$\begin{gathered} t/(^\circ C) = 1011 + 0.50[AlF_3 ] - 0.13[AIF_3 ] - \frac{{3.45[CaF_2 ]}}{{1 + 0.0173[CaF_2 ]}} \hfill \\ + 0.124[CaF_2 ] \cdot [AlF_3 ] - 0.00542([CaF_2 ] \cdot [AlF_3 ])^{1.5} \hfill \\ - \frac{{7.93[Al_2 O_3 ]}}{{1 + 0.0936[Al_2 O_3 ] - 0.0017[Al_2 O_3 ]^2 - 0.0023[AlF_3 ] \cdot [Al_2 O_3 ]}} \hfill \\ - \frac{{8.90[LiF]}}{{1 + 0.0047[LiF] + 0.0010[AlF3]^2 }} - 3.95[MgF_2 ] - 3.95 \hfill \\ \end{gathered} $$ wheret is the temperature in degree Celsius and the square brackets denote the weight percent of components in the system Na3AlF6-AlF3-CaF2-Al2O3-LiF-MgF2-KF. The composition limitations are [AlF3] ≈ [CaF2] ≈ [LiF] < 20 wt pct, [MgF2] ≈ [KF] < 5 wt pct, and [A12O3] up to saturation. 相似文献
64.
Stangegaard M Frøslev TG Frank-Hansen R Hansen AJ Morling N 《Journal of laboratory automation》2011,16(2):134-140
We have implemented and validated automated protocols for DNA extraction and PCR setup using a Tecan Freedom EVO liquid handler mounted with the Te-MagS magnetic separation device (Tecan, M?nnedorf, Switzerland). The protocols were validated for accredited forensic genetic work according to ISO 17025 using the Qiagen MagAttract DNA Mini M48 kit (Qiagen GmbH, Hilden, Germany) from fresh whole blood and blood from deceased individuals. The workflow was simplified by returning the DNA extracts to the original tubes minimizing the risk of misplacing samples. The tubes that originally contained the samples were washed with MilliQ water before the return of the DNA extracts. The PCR was setup in 96-well microtiter plates. The methods were validated for the kits: AmpF?STR Identifiler, SGM Plus and Yfiler (Applied Biosystems, Foster City, CA), GenePrint FFFL and PowerPlex Y (Promega, Madison, WI). The automated protocols allowed for extraction and addition of PCR master mix of 96 samples within 3.5h. In conclusion, we demonstrated that (1) DNA extraction with magnetic beads and (2) PCR setup for accredited, forensic genetic short tandem repeat typing can be implemented on a simple automated liquid handler leading to the reduction of manual work, and increased quality and throughput. 相似文献
65.
Björn Schuller 《International Journal of Speech Technology》2011,14(2):77-87
Little is known about the impact of reverberation on performance of running speaker state classification systems. This study
thus aims to approach the topic by measuring effects on a state-of-the-art engine with consideration of six public room impulse
responses for convolution of the affective speech signals of three standard datasets comprising of emotion and interest. Speech
data thereby is given by this year’s INTERSPEECH Paralinguistic Challenge corpus TUM AVIC and the frequently used Berlin and
eNTERFACE sets. The room impulse responses comprise rooms in private apartments, chapels, a factory hall, and a van. Speaker
independent performance after speaker adaptation is investigated. To cope with reverberation, matched condition learning and
acoustic space adaptation are considered as efficient means. By that a report is provided on suitability of feature types
given the type of impulse response. In the result almost all occurring corruption arising from reverberation can be restored,
yet the general impact varies with the type of room or acoustic environment. 相似文献
66.
We have implemented a compiler for key parts of Modelica, an object-oriented language supporting equation-based modeling and simulation of complex physical systems. The compiler is extensible, to support experiments with emerging tools for physical models. To achieve extensibility, the implementation is done declaratively in JastAdd, a metacompilation system supporting modern attribute grammar mechanisms such as reference attributes and nonterminal attributes.This paper reports on experiences from this implementation. For name and type analyses, we illustrate how declarative design strategies, originally developed for a Java compiler, could be reused to support Modelica’s advanced features of multiple inheritance and structural subtyping. Furthermore, we present new general design strategies for declarative generation of target ASTs from source ASTs. We illustrate how these strategies are used to resolve a generics-like feature of Modelica called modifications, and to support flattening, a fundamental part of Modelica compilation. To validate that the approach is practical, we have compared the execution speed of our compiler to two existing Modelica compilers. 相似文献
67.
Animated meshes are often represented by a sequence of static meshes with constant connectivity. Due to their frame-based representation they usually occupy a vast amount of bandwidth or disk space. We present a fast and efficient scalable predictive coding (SPC) scheme for frame-based representations of animated meshes. SPC decomposes animated meshes in spatial and temporal layers which are efficiently encoded in one pass through the animation. Coding is performed in a streamable and scalable fashion. Dependencies between neighbouring spatial and temporal layers are predictively exploited using the already encoded spatio-temporal neighbourhood. Prediction is performed in the space of rotation-invariant coordinates compensating local rigid motion. SPC supports spatial and temporal scalability, and it enables efficient compression as well as fast encoding and decoding. Parts of SPC were adopted in the MPEG-4 FAMC standard. However, SPC significantly outperforms the streaming mode of FAMC with coding gains of over 33%, while in comparison to the scalable FAMC, SPC achieves coding gains of up to 15%. SPC has the additional advantage over FAMC of achieving real-time encoding and decoding rates while having only low memory requirements. Compared to some other non-scalable state-of-the-art approaches, SPC shows superior compression performance with gains of over 16% in bit-rate. 相似文献
68.
We consider interconnections of locally input-to-state stable (LISS) systems. The class of LISS systems is quite large, in particular it contains input-to-state stable (ISS) and integral input-to-state stable (iISS) systems.Local small-gain conditions both for LISS trajectory and Lyapunov formulations guaranteeing LISS of the composite system are provided in this paper. Notably, estimates for the resulting stability region of the composite system are also given. This in particular provides an advantage over the linearization approach, as will be discussed. 相似文献
69.
With motivation from considerations in XML database theory and model checking, data strings have been introduced as an extension of finite alphabet strings which carry, at each position, a symbol and a data value from an infinite domain. Previous work has shown that it is difficult to come up with an expressive yet decidable automaton model for data languages. Recently, such a model, data automata, was introduced. This paper introduces a simpler but equivalent model and investigates its expressive power, algorithmic and closure properties, and some extensions. 相似文献
70.
Jon Olav Hauglid Norvald H. Ryeng Kjetil Nørvåg 《Distributed and Parallel Databases》2010,28(2-3):157-185
In distributed database systems, tables are frequently fragmented and replicated over a number of sites in order to reduce network communication costs. How to fragment, when to replicate and how to allocate the fragments to the sites are challenging problems that has previously been solved either by static fragmentation, replication and allocation, or based on a priori query analysis. Many emerging applications of distributed database systems generate very dynamic workloads with frequent changes in access patterns from different sites. In such contexts, continuous refragmentation and reallocation can significantly improve performance. In this paper we present DYFRAM, a decentralized approach for dynamic table fragmentation and allocation in distributed database systems based on observation of the access patterns of sites to tables. The approach performs fragmentation, replication, and reallocation based on recent access history, aiming at maximizing the number of local accesses compared to accesses from remote sites. We show through simulations and experiments on the DASCOSA distributed database system that the approach significantly reduces communication costs for typical access patterns, thus demonstrating the feasibility of our approach. 相似文献