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By methods of DOT hybridization and polymerase chain reaction (PCR) structures similar to Alu repeats were shown in the genome of fish and lamprey. The fact of the presence of such sections in representatives of many orders of fishes, and also our experimental data allow to suppose that Alu-like structures, similar to 3' region of Alu repeats on mammals, have been already formed in these. The evolutionary significance of described structures has been supposed. 相似文献
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Using total quality management principles within a shared governance framework, this case study discusses how to design and implement a plan to achieve independent nursing practice when an employee has unique needs. This experience demonstrates the importance of looking at root causes, separating ability and compliance issues, using all available resources creatively, and planning for success. 相似文献
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AR Reilein IS Tint NI Peunova GN Enikolopov VI Gelfand 《Canadian Metallurgical Quarterly》1998,142(3):803-813
We used melanophores, cells specialized for regulated organelle transport, to study signaling pathways involved in the regulation of transport. We transfected immortalized Xenopus melanophores with plasmids encoding epitope-tagged inhibitors of protein phosphatases and protein kinases or control plasmids encoding inactive analogues of these inhibitors. Expression of a recombinant inhibitor of protein kinase A (PKA) results in spontaneous pigment aggregation. alpha-Melanocyte-stimulating hormone (MSH), a stimulus which increases intracellular cAMP, cannot disperse pigment in these cells. However, melanosomes in these cells can be partially dispersed by PMA, an activator of protein kinase C (PKC). When a recombinant inhibitor of PKC is expressed in melanophores, PMA-induced pigment dispersion is inhibited, but not dispersion induced by MSH. We conclude that PKA and PKC activate two different pathways for melanosome dispersion. When melanophores express the small t antigen of SV-40 virus, a specific inhibitor of protein phosphatase 2A (PP2A), aggregation is completely prevented. Conversely, overexpression of PP2A inhibits pigment dispersion by MSH. Inhibitors of protein phosphatase 1 and protein phosphatase 2B (PP2B) do not affect pigment movement. Therefore, melanosome aggregation is mediated by PP2A. 相似文献
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VI Korol''kov VP Krotov V Konvertino P Le?tem EV Trambovetski? J Fanton R Krisman IuV Gordeev D Evert AM Nosovski? J Konnolli 《Canadian Metallurgical Quarterly》1998,32(3):30-37
Medical practice guidelines are being developed rapidly and advocated as a crucial component in transforming and improving the delivery of health care services. However, little is known about the ability of hospitals to institute guidelines outside of experimental settings. This article approaches guideline implementation from the viewpoint of process innovation assimilation and presents specific recommendations for organizational tactics leading to successful implementation of practice guidelines. 相似文献
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GN Kremenchutski? ML Gorbunova LG Iurgel' VI Chu?ko SA Cherniaev 《Canadian Metallurgical Quarterly》1994,56(4):36-42
The paper deals with the data on biology of Aerococcus, a slightly studied group of microorganisms. Physiological-biochemical properties of Aerococcus are described, data of their distribution in nature are given. Peculiar attention is paid to the estimate of the role of Aerococcus in human microbiocenoses. As a result of the profound and all-round study of this group of microorganisms the authors have developed new bacterial drug "A-bakterin" based on the aerococcus strain. Data presented about the results of clinical tests of "A-bakterin" are presented, a possibility to use Aerococcus lysate in the elaboration of new drugs is discussed. 相似文献