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11.
12.
Svetlana V. Klinova Boris A. Katsnelson Ilzira A. Minigalieva Oksana P. Gerzen Alexander A. Balakin Ruslan V. Lisin Ksenia A. Butova Salavat R. Nabiev Oleg N. Lookin Leonid B. Katsnelson Larisa I. Privalova Daniil A. Kuznetsov Vladimir Ya. Shur Ekaterina V. Shishkina Oleg H. Makeev Irene E. Valamina Vladimir G. Panov Marina P. Sutunkova Larisa V. Nikitina Yuri L. Protsenko 《International journal of molecular sciences》2021,22(7)
Subchronic intoxication was induced in outbred male rats by repeated intraperitoneal injections with lead oxide (PbO) and/or cadmium oxide (CdO) nanoparticles (NPs) 3 times a week during 6 weeks for the purpose of examining its effects on the contractile characteristics of isolated right ventricle trabeculae and papillary muscles in isometric and afterload contractions. Isolated and combined intoxication with these NPs was observed to reduce the mechanical work produced by both types of myocardial preparation. Using the in vitro motility assay, we showed that the sliding velocity of regulated thin filaments drops under both isolated and combined intoxication with CdO–NP and PbO–NP. These results correlate with a shift in the expression of myosin heavy chain (MHC) isoforms towards slowly cycling β–MHC. The type of CdO–NP + PbO–NP combined cardiotoxicity depends on the effect of the toxic impact, the extent of this effect, the ratio of toxicant doses, and the degree of stretching of cardiomyocytes and muscle type studied. Some indices of combined Pb–NP and CdO–NP cardiotoxicity and general toxicity (genotoxicity included) became fully or partly normalized if intoxication developed against background administration of a bioprotective complex. 相似文献
13.
A regularity of the predicted sensitivities to random and proportional dye concentration errors in regard to the position of target colour has been observed for the case of dyeing acrylic with basic dyes. The sensitivity of the recipe colour to random dye concentration errors is highest for light neutral target colours and is almost negligible for dark-shade recipes. Recipes for less saturated targets are slightly more sensitive than recipes for more saturated targets of equal lightness. The span of the sensitivities to weighing error of the recipes matching a given target varies with the position of the target in colour space. By contrast, the sensitivity of recipes to dye strength error is the highest at medium to low lightness for neutral and near neutral target shades. The span of sensitivities of the recipes for any such particular target is broad, with some having low sensitivity to strength errors. Recipes for the target colours at the 'lighter' part of the gamut border were the least sensitive to strength errors. Received: 10 June 2005; Accepted: 11 July 2005. 相似文献
14.
Sophie Lakard Nadège Morrand-Villeneuve Boris Lakard Guillaume Herlem Bernard Fahys 《Electrochimica acta》2007,53(3):1114-1126
Up to today, several techniques have been used to maintain cells in culture for studying many aspects of cell biology and physiology. More often, cell culture is dependent on proper anchorage of cells to the growth surface. Thus, poly-l-lysine, fibronectin or laminin are the most commonly used substrates. In this study, electrosynthesized biocompatible polymer films are proposed as an alternative to these standard substrates. The electrosynthesized polymers tested were polyethylenimine, polypropylenimine and polypyrrole. Then, the adhesion, proliferation and morphology of rat neuronal cell lines were investigated on these polymer substrates in an attempt to develop new and efficient polymer materials for cell culture. During their growth on the polymers, the evolution of the cell morphology was monitored using both confocal microscopy and immunohistochemistry, leading to the conclusion of a normal development. An estimation of the adhesion and proliferation rates of rat neuronal cell cultures indicated that polyethylenimine and polypropylenimine were the best substrates for culturing olfactory neuronal cells. A method to favour the differentiation of the neuronal cells was also developed since the final aim of this work is to develop a biosensor for odour detection using differentiated neuronal cells as transducers. Consequently, a biosensor was microfabricated using silicon technology. This microsystem allowed us to culture the cells on a silicon wafer and to position the cells on certain parts of the silicon wafer. 相似文献
15.
16.
Asoke C. D. Chaklader Sankar Das Gupta Edmond C. Y. Lin Boris Gutowski 《Journal of the American Ceramic Society》1992,75(8):2283-2285
Al2 O3 and SiC composite materials have been produced from mixtures of aluminosilicates (both natural minerals and synthetic) and carbon as precursor materials. These composites are produced by heating a mixture of kaolinite (or synthetic aluminosilicates) and carbon in stoichiometric proportion above 1550°C, so that only Al2 O3 and SiC remain as the major phases. A similar process has also been used for synthesizing other composite powders having mixtures of Al2 O3 , SiC, TiC, and ZrO2 in different proportions (all compounds together or selective mixtures of some of them), as desired. The microstructure of hot-pressed dense compacts, produced from these powders, revealed that the SiC phase is distributed very homogeneously, even occasionally within Al2 O3 grains on a nanosize scale. The homogeneous distribution of SiC particles within the system produced high fracture toughness of the hot-pressed material (KIC ∼ 7.0 MPa · m1/2 ) and having Vicker's hardness values greater than 2000 kgf/mm2 . 相似文献
17.
Pt/Al2O3 catalysts with platinum contents of 0.1, 1.0 and 3.0 wt% were used for the low temperature combustion of methanol in the absence and the respective presence of a trace amount of ammonia. It is observed that ammonia inhibits the combustion of methanol, which is due to competition between methanol and ammonia for the same sites. For a fixed space velocity and without ammonia in the gas, the performances of the catalysts increase up to a Pt loading of 1.0 wt%. In the presence of ammonia, however, no upper limit of performance is observed with increased Pt content. The results are discussed in terms of both Pt–support interface and the Pt surface being active. 相似文献
18.
Electrophoretic deposition of carbon nanotubes 总被引:1,自引:0,他引:1
Aldo R. Boccaccini Johann Cho Judith A. Roether Boris J.C. Thomas E. Jane Minay Milo S.P. Shaffer 《Carbon》2006,44(15):3149-3160
Electrophoretic deposition (EPD) has been gaining increasing interest as an economical and versatile processing technique for the production of novel coatings or films of carbon nanotubes (CNTs) on conductive substrates. The purpose of the paper is to present an up-to-date comprehensive overview of current research progress in the field of EPD of CNTs. The paper specifically reviews the preparation and characterisation of stable CNT suspensions, and the mechanism of the EPD process; it includes discussion of pure CNT coatings and CNT/nanoparticle composite films. A complete discussion of the EPD parameters is presented, including electrode materials, deposition time, electrode separation, deposition voltage and resultant electric field. The paper highlights potential applications of the resulting CNT and CNT/composite structures, in areas such as field emission devices, fuel cells, and supercapacitors. 相似文献
19.
Boris Raymond Gaël Guennebaud Pascal Barla Romain Pacanowski Xavier Granier 《Computer Graphics Forum》2014,33(2):313-321
This paper introduces a system for the direct editing of highlights produced by anisotropic BRDFs, which we call anisotropic highlights. We first provide a comprehensive analysis of the link between the direction of anisotropy and the shape of highlight curves for arbitrary object surfaces. The gained insights provide the required ingredients to infer BRDF orientations from a prescribed highlight tangent field. This amounts to a non‐linear optimization problem, which is solved at interactive framerates during manipulation. Taking inspiration from sculpting software, we provide tools that give the impression of manipulating highlight curves while actually modifying their tangents. Our solver produces desired highlight shapes for a host of lighting environments and anisotropic BRDFs. 相似文献
20.
The metabolism of 1-acyl-2-acetyl-sn-glycero-3-phosphocholine (1-acyl-PAF), a naturally occurring analogue of platelet activating factor (PAF), was investigated
in rabbit platelets. Our studies showed that 1-acyl-[3H]PAF (1-palmitoyl-2-acetyl-sn-glycero-3-phospho[N-methyl-3H]-choline) was converted by platelets into phosphatidyl-[3H]choline ([3H]PC) in a time-dependent fashion. The formation of [3H]PC occurred at a rate similar to that observed when lyso-[3H]PC (palmitoyl-sn-glycero-3-phospho[N-methyl-3H]choline) was used as substrate. In addition, a time-dependent increase in the level of water-soluble radioactivity was observed
during the incubation of platelets with either 1-acyl-[3H]PAF or lyso-[3H]PC. This increase was parallel to the formation of [3H]PC and was not observed in the presence of [14C]PAF (1-octadecyl-2-acetyl-sn-glycerol-3-phospho[N methyl-14C]choline). Analysis by thin-layer chromatography showed that the soluble radioactivity was mainly associated with glycerophosphocholine
(GPC). On the other hand, the preincubation of platelets with phenylmethylsulfonyl fluoride, an inhibitor of the acetylhydrolase,
reduced the hydrolysis of 1-acyl-[3H]PAF to [3H]GPC with a concomitant accumulation of radioactivity in 1-acyl-PAF. These findings suggest that 1-acyl-PAF is converted
into PC through deacetylation-reacylation with lysoPC as an obligatory intermediate. The findings also indicate that the lysoPC
resulting from 1-acyl-PAF is either reacylated to phosphatidylcholine (PC) or hydrolyzed to GPC by lysophospholipase. Finally,
we showed that the stimulation of platelets with PAF led to a time- and concentration-dependent increase in the conversion
of 1-acyl-[3H]PAF to [3H]PC. The stimulatory effect of PAF was not observed when platelets were lysed before incubation, suggesting that PAF enhances
the metabolism of 1-acyl-PAF, probably by accelerating its translocation through the plasma membrane. 相似文献