Partial preservation of pancreatic beta-cells by vanadium: evidence for long-term amelioration of diabetes

Streptozotocin (STZ)-diabetic rats treated with vanadium can remain euglycemic for up to 20 weeks following withdrawal from vanadium treatment. In this study, we examined the effects of short-term vanadium treatment in preventing or reversing the STZ-induced diabetic state. Male Wistar rats were untreated (D) or treated (DT) with vanadyl sulfate for 1 week before administering STZ. Treatment was subsequently maintained for 3 days (DT3) or 14 days (DT14) post-STZ, after which vanadium was withdrawn. At 4 to 5 weeks post-STZ and following long-term withdrawal from vanadium, DT14 rats demonstrated levels of food and fluid intake and glucose tolerance that were not significantly different from those of age-matched untreated nondiabetic rats, and had significantly reduced glycemic levels in the fed state compared with D and DT3 groups. The proportion of animals that were euglycemic (fed plasma glucose < 9.0 mmol/L) was significant in DT14 (five of 10) relative to D (one of 10) and DT3 (one of 10) (P = .01). All euglycemic animals had an improved pancreatic insulin content that, albeit low (12% of control), was strongly linked to euglycemia in the fed state (r = -.91, P < .0001). Moreover, the highly significant correlation persisted with the analysis of untreated STZ-rats alone (r = -.95, P < .0001). Similarly, improvements in glucose tolerance and insulin secretory function in euglycemic rats were strongly correlated with small changes in residual insulin content. Hence, as vanadium pretreatment did not prevent STZ-induced beta-cytotoxicity, the vanadium-induced amelioration of the diabetic state appears to be secondary to the preservation of a functional portion of pancreatic beta cells that initially survived STZ toxicity. The partial preservation of pancreatic beta cells, albeit small in proportion to the total insulin store, was both critical and sufficient for a long-term reversal of the diabetic state. These results suggest that apparently modest effects in preserving residual pancreatic insulin content can have profound consequences on glucose homeostasis and may bear important implications for interventions that have "limited" protective effects on beta cells.     

Protein identification using 20-minute Edman cycles and sequence mixture analysis

We have developed a 20-min Edman cycle and a multiple sample horizontal flow reactor for the sequence analysis of PVDF-electroblotted proteins. The 20-min cycle uses a 12-min C18 phenylthiohydantoin separation. This cycle and separation is compatible with most Applied Biosystems sequencers. Using this rapid cycle, 10 residues on six different proteins can be completed within a 24-h period. We also demonstrate the use of an algorithm that can sort mixture sequences derived from PVDF bands that contain coeluting proteins.     

Influence of mechanical tolerances on field quality in the LHC maindipoles

We evaluate the influence of mechanical tolerances in the field quality in the LHC dipoles. We show that the most relevant effect is due to tolerances on the coil and on the internal part of the collars. The sensitivities of the field error multipoles on the mechanical tolerances are worked out using a finite element model of the dipole cross section. A Monte Carlo method is used to simulate the overall effect of both collar and coil tolerances on field quality. Correlation between random multipoles is worked out, and a comparison with the target table of the LHC field errors is given     

Node Re-Routing and Congestion Reduction Scheme for Wireless Vehicular Networks

Recently, the interest of research is going to be focused on the emerging vehicular ad-hoc networks paradigm. In these networks, vehicles communicate with each other and have the possibility of exploiting a distributed approach, typical of ad-hoc networks, which allow mobile nodes (vehicles) to communicate with each other. Thanks to the different standards for this kind of network, such as DSRC, WAVE/IEEE802.11p, the researchers have the possibility of designing and developing new MAC and routing algorithms, trying to enhance the mobile users experience in the mobile environment. In this paper, the attention is focused on the optimization of traffic flowing in a vehicular environment with vehicle-2-roadside capability. The proposed idea exploits the information that is gathered by road-side units with the main aim of redirecting traffic flows (in terms of vehicles) to less congested roads, with an overall system optimization, also in terms of Carbon Dioxide emissions reduction. A deep campaign of simulations has been carried out to give more effectiveness to our proposal.     

EURECA: European-Japanese Microcalorimeter Array

The EURECA project aims to demonstrate technological readiness of a micro-calorimeter array for application in future X-ray astronomy missions, like Constellation-X, EDGE, and XEUS. The prototype instrument consists of a 5×5 pixel array of TES-based micro-calorimeters read out by two SQUID-amplifier channels using frequency-domain-multiplexing (FDM) with digital base-band feedback. The detector array is cooled by a cryogen-free cryostat consisting of a pulse tube cooler and a two stage ADR. Initial tests of the system at the PTB beam line of the BESSY synchrotron showed stable performance and an X-ray energy resolution of 1.5 eV at 250 eV for read-out of one TES-pixel only. Next step is deployment of FDM to read-out the full array. Full performance demonstration is expected end 2008.      

A novel, compact, low-cost, impulse ground-penetrating radar for nondestructive evaluation of pavements

This paper reports on the development of a novel, compact, low-cost, impulse ground-penetrating radar (GPR) and demonstrate its use for nondestructive evaluation of pavement structures. This GPR consists of an ultrashort-monocycle-pulse transmitter (330 ps), an ultrawide-band (UWB) sampling receiver (0-6 GHz), and two UWB antennas (0.2-20 GHz)-completely designed using microwave-integrated circuits with seamless electrical connections between them. An approximate analysis is used to determine the signal loss and power budget. Performance of this GPR has been verified through the measurements of relative permittivity and thicknesses of various samples, and a good agreement between the experimental and theoretical results has been achieved.     

Relationship between serum immunoglobulin, inhalation bronchial challenges and skin tests in severe asthmatics

The relationship among inhalation bronchial challenge tests, skin tests, total serum immunoglobulin E, A, M, and G values were investigated. Positive correlation between the results of the inhalation bronchial challenges and IgG and negative correlation between the value of IgG and IgA were found but no other correlations.     

A new typhoid vaccine composed of the Vi capsular polysaccharide

Typhoid is still prevalent in many parts of the world. We reviewed all published and unpublished studies of a newly licensed vaccine composed of the Vi capsular polysaccharide of Salmonella typhi, the causative agent of the disease, which had been licensed previously outside the United States. These included observational studies and double-blind randomized studies done in the United States, Europe, and the developing world in which children and adults unexposed to typhoid or those living in endemic areas were enrolled. A single dose of 25 micrograms of the purified polysaccharide was given by intramuscular injection. The vaccine was well tolerated, inducing only minor reactions in fewer than 10% of subjects. An antibody response occurred in about 90% of subjects and lasted about 3 years. Seroconversion was shown in children as young as 2 years. Protective efficacy was evaluated in two studies conducted in areas in which typhoid is endemic; the efficacy was 55% and 75%, respectively, in adults and in children older than 5 years. The Vi vaccine compares favorably with other typhoid vaccines in regard to safety, patient compliance, immunogenicity, and efficacy. Vi polysaccharide is a well-standardized antigen that is effective in a single parenteral dose, is safer than whole-cell vaccine, and may be used in children 2 years of age or older.