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171.
Caterina Dallari Riccardo Innocenti Elena Lenci Andrea Trabocchi Francesco Saverio Pavone Caterina Credi 《International journal of molecular sciences》2022,23(10)
Surface-enhanced Raman spectroscopy (SERS) exploiting Raman reporter-labeled nanoparticles (RR@NPs) represents a powerful tool for the improvement of optical bio-assays due to RRs’ narrow peaks, SERS high sensitivity, and potential for multiplexing. In the present work, starting from low-cost and highly available raw materials such as cysteamine and substituted benzoic acids, novel bioorthogonal RRs, characterized by strong signal (103 counts with FWHM < 15 cm−1) in the biological Raman-silent region (>2000 cm−1), RRs are synthesized by implementing a versatile, modular, and straightforward method with high yields and requiring three steps lasting 18 h, thus overcoming the limitations of current reported procedures. The resulting RRs’ chemical structure has SH-pendant groups exploited for covalent conjugation to high anisotropic gold-NPs. RR@NPs constructs work as SERS nanoprobes demonstrating high colloidal stability while retaining NPs’ physical and vibrational properties, with a limit of detection down to 60 pM. RR@NPs constructs expose carboxylic moieties for further self-assembling of biomolecules (such as antibodies), conferring tagging capabilities to the SERS nanoprobes even in heterogeneous samples, as demonstrated with in vitro experiments by transmembrane proteins tagging in cell cultures. Finally, thanks to their non-overlapping spectra, we envision and preliminary prove the possibility of exploiting RR@NPs constructs simultaneously, aiming at improving current SERS-based multiplexing bioassays. 相似文献
172.
Maria Consiglia Trotta Carlo Gesualdo Francesco Petrillo Caterina Claudia Lepre Alberto Della Corte Giancuomo Cavasso Giulia Maggiore Anca Hermenean Francesca Simonelli Michele DAmico Settimio Rossi 《International journal of molecular sciences》2022,23(9)
The most frequent retinal diseases, such as diabetic retinopathy, age-related macular degeneration and posterior uveitis, are underlined by oxidative stress or aging-induced retinal inflammation, which contributes to vision impairing or loss. Resolution of inflammation is emerging as a critical phase able to counteract the inflammatory process leading to the progression of retinal damage. Particularly, pro-resolving mediators (PMs) play a key role in the modulation of inflammatory exudates and could be considered a new target to be investigated in different inflammatory-autoimmune pathologies. Here, we highlight the most recent studies concerning the role of the main PMs (lipoxins, resolvins, prtectins, maresins and annexins) in retinal inflammation, in order to collect the best evidence in the field of inflammatory retinal damage resolution and to propose novel pharmacological approaches in the management of the most common retinal diseases. 相似文献
173.
Paola Matarrese Sonia Maccari Barbara Ascione Rosa Vona Vanessa Vezzi Tonino Stati Maria Cristina Gr Giuseppe Marano Caterina Ambrosio Paola Molinari 《International journal of molecular sciences》2022,23(9)
Adrenergic receptors (AR) belong to the G protein-coupled receptor superfamily and regulate migration and proliferation in various cell types. The objective of this study was to evaluate whether β-AR stimulation affects the antiproliferative action of α2-AR agonists on B16F10 cells and, if so, to determine the relative contribution of β-AR subtypes. Using pharmacological approaches, evaluation of Ki-67 expression by flow cytometry and luciferase-based cAMP assay, we found that treatment with isoproterenol, a β-AR agonist, increased cAMP levels in B16F10 melanoma cells without affecting cell proliferation. Propranolol inhibited the cAMP response to isoproterenol. In addition, stimulation of α2-ARs with agonists such as clonidine, a well-known antihypertensive drug, decreased cancer cell proliferation. This effect on cell proliferation was suppressed by treatment with isoproterenol. In turn, the suppressive effects of isoproterenol were abolished by the treatment with either ICI 118,551, a β2-AR antagonist, or propranolol, suggesting that isoproterenol effects are mainly mediated by the β2-AR stimulation. We conclude that the crosstalk between the β2-AR and α2-AR signaling pathways regulates the proliferative activity of B16F10 cells and may therefore represent a therapeutic target for melanoma therapy. 相似文献
174.
Qihui Yang Don M. Gruenbacher Caterina M. Scoglio 《Journal of the Royal Society Interface》2022,19(188)
After one pandemic year of remote or hybrid instructional modes, universities struggled with plans for an in-person autumn (fall) semester in 2021. To help inform university reopening policies, we collected survey data on social contact patterns and developed an agent-based model to simulate the spread of severe acute respiratory syndrome coronavirus 2 in university settings. Considering a reproduction number of R0 = 3 and 70% immunization effectiveness, we estimated that at least 80% of the university population immunized through natural infection or vaccination is needed for safe university reopening with relaxed non-pharmaceutical interventions (NPIs). By contrast, at least 60% of the university population immunized through natural infection or vaccination is needed for safe university reopening when NPIs are adopted. Nevertheless, attention needs to be paid to large-gathering events that could lead to infection size spikes. At an immunization coverage of 70%, continuing NPIs, such as wearing masks, could lead to a 78.39% reduction in the maximum cumulative infections and a 67.59% reduction in the median cumulative infections. However, even though this reduction is very beneficial, there is still a possibility of non-negligible size outbreaks because the maximum cumulative infection size is equal to 1.61% of the population, which is substantial. 相似文献
175.
Jacopo Manso Loris Bertazza Susi Barollo Alberto Mondin Simona Censi Sofia Carducci Alfonso Massimiliano Ferrara Isabella Merante Boschin Stefania Zovato Francesca Schiavi Michele Gregianin Gianmaria Pennelli Maurizio Iacobone Caterina Mian 《International journal of molecular sciences》2022,23(5)
Pheochromocytoma (Pheo) is a tumor derived from chromaffin cells. It can be studied using 18F-dihydroxyphenylalanine (DOPA)—positron emission tomography (PET) due to its overexpression of L-type amino acid transporters (LAT1 and LAT2). The oncogenic pathways involved are still poorly understood. This study examined the relationship between 18F-DOPA-PET uptake and LAT1 expression, and we explored the role of miR-375 and putative target genes. A consecutive series of 58 Pheo patients were retrospectively analyzed, performing 18F-DOPA-PET in 32/58 patients. Real-time quantitative PCR was used to assess the expression of LAT1, LAT2, phenylethanolamine N-methyltransferase (PNMT), miR-375, and the major components of the Hippo and Wingless/Integrated pathways. Principal germline mutations associated with hereditary Pheo were also studied. Pheo tissues had significantly higher LAT1, LAT2, and PNMT mRNA levels than normal adrenal tissues. MiR-375 was strongly overexpressed. Yes-associated protein 1 and tankyrase 1 were upregulated, while beta-catenin, axin2, monocarboxylate transporter 8, and Frizzled 8 were downregulated. A positive relationship was found between 18F-DOPA-PET SUV mean and LAT1 gene expression and for 24 h-urinary norepinephrine and LAT1. This is the first experimental evidence of 18F-DOPA uptake correlating with LAT1 overexpression. We also demonstrated miR-375 overexpression and downregulated (Wnt) signaling and identified the Hippo pathway as a new potentially oncogenic feature of Pheo. 相似文献
176.
Pasquale Pisapia Antonino Iaccarino Caterina De Luca Gennaro Acanfora Claudio Bellevicine Roberto Bianco Bruno Daniele Luisa Ciampi Marco De Felice Teresa Fabozzi Luigi Formisano Pasqualina Giordano Cesare Gridelli Giovanni Pietro Ianniello Annamaria Libroia Paolo Maione Mariantonia Nacchio Fabio Pagni Giovanna Palmieri Francesco Pepe Gianluca Russo Maria Salatiello Antonio Santaniello Rachele Scamarcio Davide Seminati Michele Troia Giancarlo Troncone Elena Vigliar Umberto Malapelle 《International journal of molecular sciences》2022,23(15)
Background: Immune-checkpoint inhibitors (ICIs) have increased and improved the treatment options for patients with non-oncogene-addicted advanced stage non-small cell lung cancer (NSCLC). However, the role of ICIs in oncogene-addicted advanced stage NSCLC patients is still debated. In this study, in an attempt to fill in the informational gap on the effect of ICIs on other driver mutations, we set out to provide a molecular landscape of clinically relevant oncogenic drivers in programmed death-ligand 1 (PD-L1) positive NSCLC patients. Methods: We retrospectively reviewed data on 167 advanced stage NSCLC PD-L1 positive patients (≥1%) who were referred to our clinic for molecular evaluation of five driver oncogenes, namely, EGFR, KRAS, BRAF, ALK and ROS1. Results: Interestingly, n = 93 (55.7%) patients showed at least one genomic alteration within the tested genes. Furthermore, analyzing a subset of patients with PD-L1 tumor proportion score (TPS) ≥ 50% and concomitant gene alterations (n = 8), we found that n = 3 (37.5%) of these patients feature clinical benefit with ICIs administration, despite the presence of a concomitant KRAS gene alteration. Conclusions: In this study, we provide a molecular landscape of clinically relevant biomarkers in NSCLC PD-L1 positive patients, along with data evidencing the clinical benefit of ICIs in patient NSCLC PD-L1 positive alterations. 相似文献
177.
Influence of pig genetic type on sensory properties and consumer acceptance of Parma,San Daniele and Toscano dry‐cured hams
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178.
In the continuing development of Soviet psychology, a 1968 decree provides for the awarding of advanced degrees in the psychological sciences, thus recognizing psychology as a science in its own right. A full translation of this historically significant decree is provided. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
179.
Presents information on the organization of the Society of the Psychologists of the USSR, including the structure of the governing body, the divisions, and the levels of organization. (0 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
180.
We consider the computational power of constant width polynomial size cylindrical circuits and nondeterministic branching
programs. We show that every function computed by a
circuit can also be computed by a constant width polynomial size cylindrical nondeterministic branching program (or cylindrical
circuit) and that every function computed by a constant width polynomial size cylindrical circuit belongs to ACC0. 相似文献