Maskless writing of microfluidics: Rapid prototyping of 3D microfluidics using scratch on a polymer substrate

We present a new rapid prototyping method designed for simple fabrication of 3D microfluidics using a maskless direct writing technique on polymer substrates. The entire process is enabled by a commercial cutter plotter with 10 μm resolution precision and high speed. A CAD design of top and bottom microstructures is directly written on a polymer substrate using a cutter plotter after setting up the suitable force. The smallest channel width of 20 μm was obtained with the minimum force and 100 μm from the maximum. Also the written depth increased linearly with force from 30 to 130 μm. Several 3D microfluidic devices are demonstrated using a maskless writing technique. The entire fabrication process from CAD layout to a final 3D device can be completed in 30 min outside the clean room facilities.     

Comprehensive Automation for Specialty Crops: Year 1 results and lessons learned

Comprehensive Automation for Specialty Crops is a project focused on the needs of the specialty crops sector, with a focus on apples and nursery trees. The project’s main thrusts are the integration of robotics technology and plant science; understanding and overcoming socio-economic barriers to technology adoption; and making the results available to growers and stakeholders through a nationwide outreach program. In this article, we present the results obtained and lessons learned in the first year of the project with a reconfigurable mobility infrastructure for autonomous farm driving. We then present sensor systems developed to enable three real-world agricultural applications—insect monitoring, crop load scouting, and caliper measurement—and discuss how they can be deployed autonomously to yield increased production efficiency and reduced labor costs.     

A Quantum Monte Carlo method at fixed energy

In this paper we explore ways to study the zero temperature limit of quantum statistical mechanics using Quantum Monte Carlo simulations. We develop a Quantum Monte Carlo method in which one fixes the ground state energy as a parameter. The Hamiltonians we consider are of the form H=H₀+λV with ground state energy E. For fixed H₀ and V, one can view E as a function of λ whereas we view λ as a function of E. We fix E and define a path integral Quantum Monte Carlo method in which a path makes no reference to the times (discrete or continuous) at which transitions occur between states. For fixed E we can determine λ(E) and other ground state properties of H.     

Top-down proteomic analysis by MALDI-TOF profiling: Concentration-independent biomarkers

Although numerous protein biomarkers have been correlated with advanced disease states, no new clinical assays have been developed. Goals often anticipate disease-specific protein changes that exceed values among healthy individuals, a property common to acute phase reactants. This review considers somewhat different approaches. It focuses on intact protein isoform ratios that present a biomarker without change in the total concentration of the protein. These will seldom be detected by peptide level analysis or by most antibody-based assays. For example, application of an inexpensive method to large sample groups resulted in observation of several polymorphisms, including the first structural polymorphism of apolipoprotein C1. Isoform distribution of this protein was altered and was eventually linked to increased obesity. Numerous other protein isoforms included C- and N-terminal proteolysis, changes of glycoisoform ratios and certain types of sulfhydryl oxidation. While many of these gave excellent statistical correlation with advanced disease, clinical utility was not apparent. More important may be that protein isoform ratios were very stable in each individual. Diagnosis by longitudinal analysis of the same individual might increase sensitivity of protein biomarkers by 20-fold or more. Protein changes that exceed the range of values found among healthy individuals may be uncommon.     

The effects of lumbar massage on muscle fatigue, muscle oxygenation, low back discomfort, and driver performance during prolonged driving

An increasing dependence of society on automobiles for both work and leisure and the corresponding increase in time spent seated in the car has been correlated with a greater risk of low back pain and absence from work (Porter and Gyi 2002). This study examined the effects of three types of lumbar massage units on seating comfort, muscle fatigue, muscle oxygenation, muscle blood flow and driving performance during a 1 h simulated driving task. Electromyographic (EMG) signals were recorded from the right and left thoracic and lumbar erector spinae musculature. Average EMG (AEMG), mean power frequency (MPF), gaps and amplitude probability distribution function (APDF) parameters were analysed from the three massage seats and compared to a control seat. Near infrared spectroscopy (NIRS) and skin temperature from the right thoracic and lumbar erector spinae were used as an indication of muscle oxygenation and blood flow throughout the driving task. Ratings of perceived discomfort were used to assess driver discomfort, and driving performance was assessed by calculating mean lap times for the duration of each driving trial. The results showed statistically significant increases in skin temperature compared with the control seat after 60 min of driving. The NIRS results reflected these trends although the results were not statistically significant. AEMG and MPF measures showed no significant differences between the seats. MPF measures were found to increase over time, effects attributed to increases in muscle temperature. Gaps and APDF analyses revealed greater rest times and lower activation levels, respectively, with the control seat, which could result in increased loading of passive structures. This study demonstrated the beneficial effects of lumbar massage systems in increasing muscle blood flow and oxygenation. Although EMG parameters were not significantly different, the trends support the significant blood flow results. Future research should include longer driving times and adjustments in EMG measures to account for the effects of increasing muscle temperature on AEMG and MPF measures.     

TreePlus: interactive exploration of networks with enhanced tree layouts

Despite extensive research, it is still difficult to produce effective interactive layouts for large graphs. Dense layout and occlusion make food Webs, ontologies and social networks difficult to understand and interact with. We propose a new interactive visual analytics component called TreePlus that is based on a tree-style layout. TreePlus reveals the missing graph structure with visualization and interaction while maintaining good readability. To support exploration of the local structure of the graph and gathering of information from the extensive reading of labels, we use a guiding metaphor of "plant a seed and watch it grow." It allows users to start with a node and expand the graph as needed, which complements the classic overview techniques that can be effective at (but often limited to) revealing clusters. We describe our design goals, describe the interface and report on a controlled user study with 28 participants comparing TreePlus with a traditional graph interface for six tasks. In general, the advantage of TreePlus over the traditional interface increased as the density of the displayed data increased. Participants also reported higher levels of confidence in their answers with TreePlus and most of them preferred TreePlus     

Rule-based interpretation of aerial imagery

In this paper, we describe the organization of a rule-based system, SPAM, that uses map and domain-specific knowledge to interpret airport scenes. This research investigates the use of a rule-based system for the control of image processing and interpretation of results with respect to a world model, as well as the representation of the world model within an image/map database. We present results on the interpretation of a high-resolution airport scene wvhere the image segmentation has been performed by a human, and by a region-based image segmentation program. The results of the system's analysis is characterized by the labeling of individual regions in the image and the collection of these regions into consistent interpretations of the major components of an airport model. These interpretations are ranked on the basis of their overall spatial and structural consistency. Some evaluations based on the results from three evolutionary versions of SPAM are presented.     

A prological definition of HASL a purely functional language with unification based conditional binding expressions

We present a definition in Prolog of a new purely funtional (applicative) language HASL (HArvey’s StaticLanguage). HASL is a descendant of Turner’s SASL and differs from the latter in several significant points: it includes Abramson’s unification based conditional binding constructs; it restricts each clause in a definition of a HASL function to have the same arity, thereby complicating somewhat the compilation of clauses to combinators, but simplifying considerably the HASL reduction machine; and it includes the single element domain {fail} as a component of the domain of HASL data structures. It is intended to use HASL to express the functional dependencies in a translator writing system based on denotational semantics, and to study the feasibility of using HASL as a functional sublanguage of Prolog or some other logic programming language. Regarding this latter application we suggest that since a reduction mechanism exists for HASL, it may be easier to combine it with a logic programming language than it was for Robinson and Siebert to combine LISP and LOGIC into LOGLISP: in that case a fairly complex mechanism had to be invented to reduce uninterpreted LOGIC terms to LISP values. The definition is divided into four parts. The first part defines the lexical structure of the language by means of a simple Definite Clause Grammar which relates character strings to “token” strings. The second part defines the syntactic structure of the language by means of a more complex Definite Clause Grammar and relates token strings to a parse tree. The third part is semantic in nature and translates the parse tree definitions and expressions to a variable-free string of combinators and global names. The fourth part of the definition consists of a set of Prolog predicates which specifies how strings of combinators and global names are reduced to “values”, ie., integers, truth values, characters, lists, functions, fail, and has an operational flavour: one can think of this fourth part as the definition of a normal order reduction machine.     

The influence of alcohol on the persistent effects on human performance of the hypnotics Mandrax and Nitrazepam

Psychotropic drugs are prescribed to modify human behavior but they have persistent central sedative activity which may become a troublesome side-effect. Alcohol is known to interact with psychotropes, often to potentiate their central effects. We have previously shown that residues of some hypnotic drugs persist in the body for up to a week after a single therapeutic dose and have demonstrated that alcohol decreases the elimination rate of methaqualone even when taken 2 or 3 days after the drug. We have therefore looked at the influence of alcohol on the residual effects of Mandrax and nitrazepam on three measurements of human performance. The study was a double-blind, 3-way, cross-over study in which the following treatments were used: drug + alcohol, drug + alcohol placebo, and drug placebo + alcohol. In each case the alcohol or alcohol placebo was given 1, 2, and 3 days after the drug or placebo. The subject's kinetic visual acuity was measured 40 minutes after the alcohol had been taken. This was followed by a Stroop test. Changes in mental state and arousal were measured by an 18-item visual analogue scale. An interaction between alcohol and Mandrax was apparent in the results obtained from the Stroop and visual analogue scale on the third day. However, no interaction with nitrazepam could be clearly demonstrated by any of the tests on any occasion. The application of these results to the "real life" situation is discussed.