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排序方式: 共有107条查询结果,搜索用时 15 毫秒
11.
Kouki Ishii Yoshihiro Mizutani Yoshiro Suzuki Yoichiro Koga Ryosuke Matsuzaki 《Advanced Composite Materials》2019,28(4):383-395
Three-dimensional (3D) printing is an attractive technology to produce complex structures without the need for expensive tools and molds. Additives are usually incorporated with the plastic materials used in 3D printing to increase their strength and rigidity. In particular, carbon fiber-reinforced plastic (CFRP) shows promise as a material for use in 3D printing. However, the strength of CFRP after printing is still unclear, although it is known that its strength is affected by the plastic melting during printing. In this study, we analyzed the fracture behavior of CFRP specimens before and after bending to different curvature radii. From the experimental results, a fracture criterion that described the behavior of the materials by considering tensile and compressive loads was developed. The fracture mechanism was the same for CFRP specimens with different curvature radii. These results increase our understanding of the mechanical properties of CFRP materials used in 3D printing. 相似文献
12.
Karima Rabaani Noureddine Boulejfen Ammar B. Kouki Fadhel M. Ghannouchi 《国际射频与微波计算机辅助工程杂志》2020,30(4)
A novel, low complexity approach for the analysis of nonuniform lossy substrate‐integrated waveguide transmission lines based on the method of moments is proposed. The approach uses frequency‐dependent basis functions derived from the structure's propagation characteristics. Two tapered structures are analyzed, fabricated, and measured to validate the proposed approach. The analytical results of the proposed approach for both structures are compared to those obtained by measurement and by three‐dimensional field simulation. Excellent agreement is observed between the three sets of results with simulation time savings on more than 98% and memory requirement reduction of more than 97%. 相似文献
13.
GFP expression by intracellular gene delivery of GFP-coding fragments using nanocrystal quantum dots
Hoshino A Manabe N Fujioka K Hanada S Yasuhara M Kondo A Yamamoto K 《Nanotechnology》2008,19(49):495102
Gene therapy is an attractive approach to supplement a deficient gene function. Although there has been some success with specific gene delivery using various methods including viral vectors and liposomes, most of these methods have a limited efficiency or also carry a risk for oncogenesis. We herein report that quantum dots (QDs) conjugated with nuclear localizing signal peptides (NLSP) successfully introduced gene-fragments with promoter elements, which promoted the expression of the enhanced green fluorescent protein (eGFP) gene in mammalian cells. The expression of eGFP protein was observed when the QD/gene-construct was added to the culture media. The gene-expression efficiency varied depending on multiple factors around QDs, such as (1) the reading direction of the gene-fragments, (2) the quantity of gene-fragments attached on the surface of the QD-constructs, (3) the surface electronic charges varied according to the structure of the QD/gene-constructs, and (4) the particle size of QD/gene complex varied according to the structure and amounts of gene-fragments. Using this QD/gene-construct system, eGFP protein could be detected 28 days after the gene-introduction whereas the fluorescence of QDs had disappeared. This system therefore provides another method for the intracellular delivery of gene-fragments without using either viral vectors or specific liposomes. 相似文献
14.
A chirped grating segmented into partitions each having a constant blaze angle to use in a demultiplexer for coarse wavelength division multiplexing with multimode optical fibers is developed. Its designed configuration utilizes a resonance region to achieve high diffraction efficiency and large dispersion. The width, blaze angle, and diffraction order of each partition were optimized by vector diffraction analysis. The diffraction loss of the manufactured grating was less than 1.5 dB, and polarization-dependent loss was less than 0.6 dB within a wavelength width of at least 70 nm. It is confirmed that a demultiplexer with the developed chirped grating had a wide passband and low cross talk. 相似文献
15.
Yamamoto S Manabe N Fujioka K Hoshino A Yamamoto K 《IEEE transactions on nanobioscience》2007,6(1):94-98
Vitreous is transparent tissue located between the lens and the retina of the eye, thus, difficult to look at by even ophthalmological microscope. But vitreous is connected with some sight-threatening eye diseases, for example, retinal detachment, macular hole, epi-retinal membrane, and so forth. Quantum dots (QDs) have been applied to a wide range of biological studies by taking advantage of their fluorescence properties. We established a novel technique of aqueous colloidal QD (ACQD) as a vitreous lesion detector. When compared with some conventional dyes used for clinical situation, i.e. fluorescein, indocyanine green, and triamcinolone acetonide, ACQD exerted a higher performance to detect a Weiss Ring. Furthermore ACQD is also effective to perform vitrectomy, an eye surgery to cut and eliminate vitreous. Some functional structures in vitreous are detected clearly when ACQD was injected into an enucleated porcine eye. We demonstrated that ACQD enabled any ophthalmic surgeon to perform vitrectomy reliably, easily, and more safely. Taken together, the ACQD-oriented vitreous staining system will promote ophthalmological science, and it will raise the cure rate of eye diseases 相似文献
16.
We studied the imaging performance of a chirped grating for a demultiplexer designed for coarse wavelength division multiplexing using a wavefront aberration analysis and the ray tracing simulation. The demultiplexer was composed of a chirped grating, cylindrical lenses, and a waveguide. The best image point and the spot shape focused by the chirped grating were effectively calculated with the wavefront aberration. We applied the aberration analysis to design a waveguide to connect branched beams to photodetectors, and we confirmed the demultiplexing performance experimentally. 相似文献
17.
Sakai K Obata K Yoshikawa M Takano R Shibata M Maeda H Mizutani A Terada K 《Drug development and industrial pharmacy》2012,38(10):1254-1261
Purpose: To design a high drug loading formulation of self-microemulsifying/micelle system. Methods: A poorly-soluble model drug (CH5137291), 8 hydrophilic surfactants (HS), 10 lipophilic surfactants (LS), 5 oils, and PEG400 were used. A high loading formulation was designed by a following stepwise approach using a high-throughput formulation screening (HTFS) system: (1) an oil/solvent was selected by solubility of the drug; (2) a suitable HS for highly loading was selected by the screenings of emulsion/micelle size and phase stability in binary systems (HS, oil/solvent) with increasing loading levels; (3) a LS that formed a broad SMEDDS/micelle area on a phase diagram containing the HS and oil/solvent was selected by the same screenings; (4) an optimized formulation was selected by evaluating the loading capacity of the crystalline drug. Aqueous solubility behavior and oral absorption (Beagle dog) of the optimized formulation were compared with conventional formulations (jet-milled, PEG400). Results: As an optimized formulation, d-α-tocopheryl polyoxyethylene 1000 succinic ester: PEG400?=?8:2 was selected, and achieved the target loading level (200?mg/mL). The formulation formed fine emulsion/micelle (49.1?nm), and generated and maintained a supersaturated state at a higher level compared with the conventional formulations. In the oral absorption test, the area under the plasma concentration-time curve of the optimized formulation was 16.5-fold higher than that of the jet-milled formulation. Conclusions: The high loading formulation designed by the stepwise approach using the HTFS system improved the oral absorption of the poorly-soluble model drug. 相似文献
18.
Yasushi Abe Kunio Matsubara Kiyoaki Sasagawa Kouki Matsuse 《IEEJ Transactions on Electrical and Electronic Engineering》2008,3(1):113-119
This paper presents a medium‐voltage inverter applying series connected general‐purpose 1.2 kV insulated gate bipolar transistors (IGBTs) as a switching device to achieve low switching losses compared to inverters applying high‐voltage IGBTs with over 3 kV rating. Gate signal synchronization, which is essential to keep the balance of collector‐emitter voltages across the IGBTs, is achieved by magnetically coupling all gate lines using a simple two‐windings transformer. In order to obtain better voltage balancing, influence of stray capacitance distribution associated with an insulating substrate in a two‐in‐one IGBT module on the voltage sharing is investigated, and an optimized layout of heat sinks for the IGBT modules is proposed. To validate some performances concerning the device losses and the voltage sharing, a 170 kVA inverter based on three 1.2 kV IGBTs connected in series is built and evaluated. The experimental results are shown. Copyright © 2007 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc. 相似文献
19.
There have been several studies on biopolymer synthesis under hydrothermal conditions. The conventional hydrothermal methods
make it possible to synthesize only a dipeptide and short oligopeptides as well as cyclo-dimer, from amino acids. As these
studies that were applied with various quenching methods suggested the importance of quenching rate from hydrothermal conditions,
rapid quenching could avoid hydrolysis of the oligomers that had already been synthesized under hydrothermal conditions. In
this study, therefore, we designed a novel hydrothermal flow reactor adopted with adiabatic expansion cooling system from
the reason that it was thought to be one of the most rapid quenching methods. It mimics geysers, fumaroles, hot springs, and
volcanic eruptions. Once aqueous solutions of monomers were treated at high temperature and pressure, the solutions were released
into the atmosphere through an orifice to be depressurized and cooled down simultaneously with the Joule–Thomson effect. We
demonstrated oligomerization of glycine up to decamer (Gly10) by using the flow reactor, which had never been yielded with any other quenching methods. This suggests that rapid quenching
methods under non-equilibrium conditions such as adiabatic expansion cooling is an efficient way to produce long oligomers
connected by covalent bonds via dehydration condensation. 相似文献
20.
Toyoda S Suzuki Y Hattori S Yamada K Fujii A Yoshida N Kouno R Murayama K Shiomi H 《Environmental science & technology》2011,45(3):917-922
Wastewater treatment processes are believed to be anthropogenic sources of nitrous oxide (N(2)O) and methane (CH(4)). However, few studies have examined the mechanisms and controlling factors in production of these greenhouse gases in complex bacterial systems. To elucidate production and consumption mechanisms of N(2)O and CH(4) in microbial consortia during wastewater treatment and to characterize human waste sources, we measured their concentrations and isotopomer ratios (elemental isotope ratios and site-specific N isotope ratios in asymmetric molecules of NNO) in water and gas samples collected by an advanced treatment system in Tokyo. Although the estimated emissions of N(2)O and CH(4) from the system were found to be lower than those from the typical treatment systems reported before, water in biological reaction tanks was supersaturated with both gases. The concentration of N(2)O, produced mainly by nitrifier-denitrification as indicated by isotopomer ratios, was highest in the oxic tank (ca. 4000% saturation). The dissolved CH(4) concentration was highest in in-flow water (ca. 3000% saturation). It decreased gradually during treatment. Its carbon isotope ratio indicated that the decrease resulted from bacterial CH(4) oxidation and that microbial CH(4) production can occur in anaerobic and settling tanks. 相似文献