The E3 Ligase GmPUB21 Negatively Regulates Drought and Salinity Stress Response in Soybean

E3-ubiquitin ligases are known to confer abiotic stress responses in plants. In the present study, GmPUB21, a novel U-box E3-ubiquitin ligase-encoding gene, was isolated from soybean and functionally characterized. The expression of GmPUB21, which possesses E3-ubiquitin ligase activity, was found to be significantly up-regulated by drought, salinity, and ABA treatments. The fusion protein GmPUB21-GFP was localized in the cytoplasm, nucleus, and plasma membrane. Transgenic lines of the Nicotiana benthamiana over-expressing GmPUB21 showed more sensitive to osmotic, salinity stress and ABA in seed germination and inhibited mannitol/NaCl-mediated stomatal closure. Moreover, higher reactive oxygen species accumulation was observed in GmPUB21 overexpressing plants after drought and salinity treatment than in wild-type (WT) plants. Contrarily, silencing of GmPUB21 in soybean plants significantly enhanced the tolerance to drought and salinity stresses. Collectively, our results revealed that GmPUB21 negatively regulates the drought and salinity tolerance by increasing the stomatal density and aperture via the ABA signaling pathway. These findings improved our understanding of the role of GmPUB21 under drought and salinity stresses in soybean.     

The characteristics of waves around an entrance of a lagoon in South China Sea

The characteristics of the waves around a lagoon entrance in South China Sea were observed during the past one and half years based on the wave measurements.The...     

洁具高压注浆成形坯料粘土成分研究的新进展

本文介绍了国外关于洁具高压注浆成形坯料粘土成分研究的新进展．为国内同行提供一定的参考作用。     

Fabrication and performance of atmospheric plasma sprayed solid oxide fuel cells with liquid antimony anodes

A solid oxide fuel cell (SOFC) with a liquid antimony anode (LAA) is a potential energy conversion technology for the use of impurity-containing fuels.Atmospher...     

基于DSP的嵌入式系统的程序引导设计

作对以DSP为核心的嵌入式系统的程序引导功能进行了深入的研究及分析,并以TI公司的TMS320C33芯片为实例,归纳了其实现流程及设计方案,从硬件和软件两个方面阐述了实现该功能的关键点和值得注意的地方。该设计已被应用于一个配电网环网柜智能终端装置。运行实践表明,是一种简便、可靠的方案。     

电机转速和转子位置数字测量方法的研究

电机转速和转子位置的测量在电机交直流调速控制系统中占有重要的位置,为提高测量的精度和避免噪声影响,多采用数字式测量方法.基于常规的M/T法,提出了电机转速和转子位置测量的综合M/T法,该方法是利用测量电路对光电编码器信号进行预处理,以确定采样信号脉冲与码盘信号脉冲之间的相对位置,并将这个相对位置表示为含有小数的码盘脉冲个数,由此不仅可以测量出电机在采样时刻的转速,同时还可以测量出该时刻电机的转子位置.与常规的M/T法相比,该方法提高了测量的精度,扩展了M/T法的应用范围.     

Milling process simulation for the variable pitch cutter based on an integrated process-machine model

Milling process simulations provide a useful way to design and adjust the technical parameters of milling process. As the milling process involves coupling     

炸药冲击波激励氩气闪光实验研究

建立了对可见光和近红外的瞬态测试记录系统。用实验方法研究了氩气在炸药冲击小励下产生的可见光光强。测得了不同压力下氩气的辐射强度,分析了气体压力对光强的影响。