Genetic marking shows that Ph+ cells present in autologous transplants of chronic myelogenous leukemia (CML) contribute to relapse after autologous bone marrow in CML

Relapse after autologous bone marrow transplantation for chronic myelogenous leukemia (CML) can be due either to the persistence of leukemia cells in systemic tissues following preparative therapy, or due to the persistence of leukemia cells in the autologous marrow used to restore marrow function after intensive therapy. To help distinguish between these two possible causes of relapse, we used safety-modified retroviruses, which contain the bacterial resistance gene NEO, to mark autologous marrow cells that had been collected from patients early in the phase of hematopoietic recovery after in vivo chemotherapy. The cells were then subjected to ex vivo CD34 selection following collection and 30% of the bone marrow were exposed to a safety-modified virus. This marrow was infused after delivery of systemic therapy, which consisted of total body irradiation (1,020 cGy), cyclophosphamide (120 mg/kg), and VP-16 (750 mg/m2). RT PCR assays specific for the bacterial NEO mRNA, which was coded for by the virus, and the bcr-abl mRNA showed that in two evaluable CML patients transplanted with marked cells, sufficient numbers of leukemia cells remained in the infused marrow to contribute to systemic relapse. In addition, both normal and leukemic cells positive for the retroviral transgenome persisted in the systemic circulation of the patients for at least 280 days posttransplant showing that the infused marrow was responsible for the return of hematopoiesis following the preparative therapy. This observation shows that it is possible to use a replication-incompetent safety-modified retrovirus in order to introduce DNA sequences into the hematopoietic cells of patients undergoing autologous bone marrow transplantation. Moreover, this data suggested that additional fractionation procedures will be necessary to reduce the probability of relapse after bone marrow transplantation in at least the advanced stages of the disease in CML patients undergoing autologous bone marrow transplantation procedures.     

共生视域下河北工业遗产数字化保护系统研究

文中从数字化的深度和广度两个方面,将工业遗产数字化保护划分为4个阶段,即基础信息化、应用数字化、全面系统化和共生视域下的智慧生态化。随后围绕共生视域,从数字化保护、文化遗产等方向进行了研究,发现可以考虑以数字孪生技术为依托,实现工业遗产数字化保护。基于此,文中搭建了一款基于数字孪生的工业遗产数字化保护系统,该系统包含感知层、数据层、运算层、功能层、应用层等。最后,以该系统为依托,构建了河北工业遗产综合服务体系,希望能为工业遗产的数字化保护提供一定的借鉴与参考。     

林口县学校周边“小饭桌”食品卫生监督管理成效初探

为加强和规范学校周边"小饭桌"的经营行为,提高卫生监督管理水平,确保学生的身体健康,对林口县学校周边"小饭桌"实施了一系列监督管理措施,取得了良好的成效,并将在日常的卫生监督管理中引入HACCP系统原理,紧抓关键控制点,以确保广大学生的饮食安全。     

致密砂岩变黏滑溜水体系的研制与应用

目的 为了满足致密砂岩气藏储层改造需求以及解决作业现场压裂返排液处理难题,开发了一种自缔合乳液变黏滑溜水(VSW)体系,该体系仅含一种多效添加剂。方法 通过含量控制实现滑溜水与携砂液的在线转变,评价了压裂液的降阻性能、耐温抗剪切性能、携砂性能、破胶液性能及岩心基质伤害,并在苏里格气田开展了水平井现场试验。结果 配方为1.0%(w)VSW的高黏滑溜水在清水和标准盐水中的黏度分别为93 mPa·s和64 mPa·s;清水配制的1.0%(w)VSW高黏滑溜水,在90℃、170 s^-1下剪切1 h后,黏度为78 mPa·s;携砂性能良好,0.425～0.850 mm陶粒支撑剂的沉降速度为0.84 mm/s。配方为0.1%(w)～0.3%(w)VSW的低黏滑溜水降阻率可超过75%。高黏滑溜水破胶液黏度为1.74 mPa·s,对岩心基质的损害率低于10%;现场压裂施工最高加砂质量浓度达700 kg/m³,平均无阻流量达104.69×10⁴ m³/d,返排液回收利用率达97.5%。结论 该体系具有良好的增黏性及抗...     

Surface Tension and Density of Bismuth Germanate Melts as a Function of Composition and Temperature

The surface tension and density of x Bi₂O₃–(1− x )GeO₂ (BGO) melts have been measured systematically from their respective melting points up to around 1480 K within 0.25≤ x ≤0.86. With the addition of Bi₂O₃, the density of this system increased smoothly with a positive curvature. Deviation of molar volume from ideality of this system melts decreased firstly, past a minimum at about x =0.4, and then increased. Partial molar volumes of Bi₂O₃ and GeO₂ have also been calculated based on the measured density data and compared with those of a binary bismuth borate system. On increasing the content of Bi₂O₃, the surface tension of BGO melts increased slowly when x <∼0.40, past a maximum at about x =0.50, and then decreased rapidly. In addition, the temperature coefficient of surface tension remained positive within the lower content range of Bi₂O₃, changed sign from positive to negative at about x =0.38, and then was almost independent of the composition within the measured temperature range when the Bi₂O₃ content was increased further.     

Preparation of adsorbent for palladium ions by amination of acrylonitrile grafted polypropylene nonwoven fabrics

A new chelating fabrics are synthesized by radiation induced grafting of acrylonitrile (AN) onto polypropylene nonwoven fabrics (PP fabrics) and subsequent conversion of cyano to an imine by reaction with ethylene diimine (EDA). Influence of various parameters such as initial concentration, pH and adsorption time on the adsorption amount for palladium ions was investigated. It was found that adsorbent achieved excellent adsorption performance in palladium ion removal in the pH range of 1.88–5.70. The adsorption amount of ions increased with shaking time and initial concentration of palladium ions. The grafted and aminated fabrics were characterized by FT‐IR, TGA and SEM. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010     

向日葵茎髓提取物的抑菌活性及机制

以向日葵茎髓为原料,制得3种不同极性向日葵茎髓提取物。采用琼脂扩散法测定3种不同极性向日葵茎髓提取物对4种受试菌的抑菌活性;并以其中的金黄色葡萄球菌和大肠杆菌为研究对象,探讨了乙酸乙酯萃取部位(EE)的抑菌机制。结果显示:不同极性萃取部位对4种受试菌均有一定抑制作用。其中,EE处理金黄色葡萄球菌和大肠杆菌的最低抑菌质量浓度(MIC)均为0.78 g/L,半数抑制质量浓度(IC50)分别为(1.11?0.01)和(1.59?0.03)g/L;抑菌作用与其总酚、总黄酮含量呈显著正相关性(P0.05)。通过SEM分析发现,EE处理引起菌体形态变化,破坏菌体的细胞膜结构;EE可增加金黄色葡萄球菌和大肠杆菌细胞膜结构的通透性,使菌体内的电解质、蛋白质、碱性磷酸酶向胞外泄露;应用4?,6-二脒基-2-苯基吲哚(DAPI)染色和荧光检测发现,EE能干扰细菌遗传物质(DNA)的复制,最终抑制受试菌增殖。     

司盘-80辅助回流法提取红藤中的黄酮类物质

采用表面活性剂辅助回流法提取红藤中的黄酮类物质,考察了表面活性剂的种类、浓度,液料比,溶剂pH值以及回流时间等因素对红藤中黄酮类物质得率的影响,并用正交实验确定最佳工艺条件为：司盘-800．007g／mL,液料比50mL／g,溶剂pH值为6,回流时间60rain,此时红藤总黄酮的得率为19．5％。与传统水提法相比,得率提高了92．74％。     

多层底水油藏层间干扰规律研究及应用 ——以秦皇岛32-6油田为例

在一些多层底水油藏注水开发中,由于油水关系复杂、纵向各层物性及流体性质差异大,因此层间矛盾突出,后期调整难度较大.以秦皇岛32-6油田为例,采用动态反演方法定量评价层间干扰变化规律.综合考虑油水关系、渗透率、有效厚度及流体等关键因素,建立了层间干扰定量预测方法;综合考虑层间干扰与启动压力的影响,建立了普通稠油油藏多层合...     

城市生活垃圾热值的特征变量选择方法及预测建模

在垃圾焚烧的过程中,垃圾热值的波动会影响垃圾焚烧的稳定性.为了实现城市生活垃圾热值的实时在线预测以及变化趋势预测,采用模糊神经网络软测量方法,利用焚烧发电厂在线运行数据作为输入,实现垃圾热值的实时预测功能.首先采用互信息方法从若干特征变量中剔除部分无关变量;然后将模糊神经网络和粒子群优化算法结合起来从上述选择出的特征变量中进一步剔除冗余变量,从而确定预测垃圾热值的输入变量,并从中训练出垃圾热值的模糊神经网络预测模型;最后通过采集的样本数据进行性能测试.结果表明该方法有较好的预测准确率和实时性,适用于垃圾热值的在线预测.