Preparation and Surface Activity of Phosphated Alkyl Oligoglucosides

A family of phosphated alkyl oligoglucoside surfactants was prepared by reacting alkyl oligoglucosides with phosphorus oxychloride. The alkyl oligoglucosides were obtained by an usual method in which the glucose is reacted with a fatty alcohol containing 10–18 carbon atoms. These novel phosphated surfactants have been found to exhibit good surface tension, foaming and wetting power. The critical micelle concentration was found to increase with the length of hydrocarbon chain of the surfactant. The surface excess concentration and the interfacial area per surfactant molecule are reported. These phosphated surfactants also exhibit a good performance to improve the whiteness and wetting of cotton fabrics in a hydrogen oxide bleaching system, and they are also found to be more biodegradable than conventional surfactants.     

Preparation,morphology, and properties of silane‐modified MWCNT/epoxy composites

Both epoxy resin and acid‐modified multiwall carbon nanotube (MWCNT) were treated with 3‐isocyanatopropyltriethoxysilane (IPTES). Scanning electron microscopy (SEM) and transmission electronic microscope (TEM) images of the MWCNT/epoxy composites have been investigated. Tensile strength of cured silane‐modified MWCNT (1.0 wt %)/epoxy composites increased 41% comparing to the neat epoxy. Young's modulus of cured silane‐modified MWCNT (0.8 wt %)/epoxy composites increased 52%. Flexural strength of cured silane‐modified MWCNT (1.0 wt %)/epoxy composites increased 145% comparing to neat epoxy. Flexural modulus of cured silane‐modified MWCNT (0.8 wt %)/epoxy composites increased 31%. Surface and volume electrical resistance of MWCNT/epoxy composites were decreased with IPTES‐MWCNT content by 2 orders and 6 orders of magnitude, respectively. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2010     

A spread spectrum clock generator with a wide frequency and attenuation tuning range based on a third-order error-feedback delta-sigma modulator

In this paper, a novel design of spread-spectrum clock generator (SSCG) with a third-order error-feedback delta-sigma modulator is presented. The proposed SSCG with triangular modulation can generate clocks with center spread ratios of 0.25, 1, 1.75, 2.5, 3.5, 5% and down spread ratios of 0.5, 2, 3.5, 5, 7, 10% over a wide frequency range from 20 to 700 MHz. The SSCG is implemented on a chip using SMIC 0.13 um CMOS process. Our tests show that 11.31 dB attenuation of the EMI at 80 MHz with down spread ratio of 10% and 12.98 dB attenuation at 133.3 MHz with center spread ratio of 5% can be achieved which is in agreement with the theoretic calculation.     

栅氧化层经时击穿物理模型应用分析

讨论了栅氧化层击穿的统计模型,并对业界广泛应用的1/E模型、E模型以及幂指数模型中栅氧化层击穿的物理机理进行了仔细研究,指出了各个模型应用的局限性,结合实验数据分析,明确给出了各个模型的应用范围。详细讨论了电场加速因子与激活能在三个模型中的不同物理含义,总结了电场加速因子、激活能随着栅氧化层厚度变化的发展趋势以及所对应的击穿机理,据此提出了通过激活能与电场加速因子选择和验证所用加速模型是否合理的方法。此方法为判断测试条件是否合理,分析测试结果的内在含义提供了更直接、有用的参考信息。     

"楔形"TEM样品的机械研磨制备技术

介绍了一种用机械研磨法制备集成电路TEM楔形样品的技术,讨论了该制样技术所需注意的关键点,并给出了判断样品薄区是否满足TEM分析要求的两种方法。楔形样品减薄技术兼具制样速度快和样品质量好的优点。该技术既可用于制备非定点TEM样品,也可用于制备定点的TEM样品。给出了用该技术制备的定点失效的MOS器件TEM照片。熟练的技术人员可以用此方法在半小时内完成一个样品的制备。     

Scalable user-friendly image sharing

Image sharing addresses a fault-tolerant protection mechanism for important images. In a typical image sharing scheme, the generated shadow images usually have a noise-like appearance that conceals the secret image completely, but this makes them difficult to identify and manage. This paper proposes a scalable user-friendly image sharing scheme in which each generated shadow image looks like a shrunken replica of the original image. The scheme provides an easy-to-identify interface for managing the shadow images. Compared with previous user-friendly image sharing schemes, the proposed method can reconstruct the original image without any loss and still has the small-size shadow images. A notable feature is that the qualities of generated shadow images are scalable in the proposed scheme, which allows the quality of the shadow images to be adjusted according to the requirements of diverse applications.     

Efficient Mode Decision Algorithm Based on Spatial,Temporal, and Inter‐layer Rate‐Distortion Correlation Coefficients for Scalable Video Coding

The layered coding structure of scalable video coding (SVC) with adaptive inter‐layer prediction causes noticeable computational complexity increments when compared to existing video coding standards. To lighten the computational complexity of SVC, we present a fast algorithm to speed up the inter‐mode decision process. The proposed algorithm terminates inter‐mode decision early in the enhancement layers by estimating the rate‐distortion (RD) cost from the macroblocks of the base layer and the enhancement layer in temporal, spatial, and inter‐layer directions. Moreover, a search range decision algorithm is also proposed in this paper to further increase the motion estimation speed by using the motion vector information from temporal, spatial, or inter‐layer domains. Simulation results show that the proposed algorithm can determine the best mode and provide more efficient total coding time saving with very slight RD performance degradation for spatial and quality scalabilities.     

Galactose Encapsulated Multifunctional Nanoparticle for HepG2 Cell Internalization

The fluorescent dye Cy3 and galactose derivatives are covalently assembled with different ratios on the surfaces of magnetic nanoparticles (MNPs) to produce multifunctional HepG2 cancer cell–targeting agents and the effect of ligand spatial orientation on the MNP surface is investigated on targeting specificity. By using a mixture of bis‐N‐hydroxysuccinimide ester (a bifunctional linker) and OSu‐activated Cy3 (w/w = 30:1), stable and quantifiable fluorescent MNPs (Cy3@MNPs) are synthesized that could be subsequently loaded with galactosyl ligands. A mono‐antennary and two different tri‐antennary galactosyl ligands are individually immobilized on Cy3@MNPs, and the uptake efficiencies of the resulting galactosyl Cy3@MNPs by HepG2 and HeLa cells are investigated using confocal microscopy. The confocal images show that galactosyl Cy3@MNPs are sprayed over cytoplasm of the HepG2 cells, indicating that the MNP uptake occurs via receptor‐mediated endocytosis that is followed by release from endosomes. The results also reveal that the ligand spatial orientation affects the efficiency of the receptor‐mediated endocytosis and one of the tri‐antennary galactosyl ligands shows the best uptake efficiency owing to the optimal spatial presentation of the galactosyl moieties. Overall, it is shown that the MNP is a good ligand carrier and that, when pre‐assembled, the multivalent ligand structure enhances the interactions between the surface ligands of the MNPs and receptors of HepG2 cells. Additionally, the galactosyl Cy3@MNPs are not cytotoxic, indicating that they may potentially be used for in vivo applications.     

Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells without Reprogramming Factor c-Myc

The only curative treatment for hepatic failure is liver transplantation. Unfortunately, this treatment has several major limitations, as for example donor organ shortage. A previous report demonstrated that transplantation of induced pluripotent stem cells without reprogramming factor c-Myc (3-genes iPSCs) attenuates thioacetamide-induced hepatic failure with minimal incidence of tumorigenicity. In this study, we investigated whether 3-genes iPSC transplantation is capable of rescuing carbon tetrachloride (CCl(4))-induced fulminant hepatic failure and hepatic encephalopathy in mice. Firstly, we demonstrated that 3-genes iPSCs possess the capacity to differentiate into hepatocyte-like cells (iPSC-Heps) that exhibit biological functions and express various hepatic specific markers. 3-genes iPSCs also exhibited several antioxidant enzymes that prevented CCl(4)-induced reactive oxygen species production and cell death. Intraperitoneal transplantation of either 3-genes iPSCs or 3-genes iPSC-Heps significantly reduced hepatic necrotic areas, improved hepatic functions, and survival rate in CCl(4)-treated mice. CCl(4)-induced hepatic encephalopathy was also improved by 3-genes iPSC transplantation. Hoechst staining confirmed the successful engraftment of both 3-genes iPSCs and 3-genes iPSC-Heps, indicating the homing properties of these cells. The most pronounced hepatoprotective effect of iPSCs appeared to originate from the highest antioxidant activity of 3-gene iPSCs among all transplanted cells. In summary, our findings demonstrated that 3-genes iPSCs serve as an available cell source for the treatment of an experimental model of acute liver diseases.     

Glucose 1‐Phosphate Thymidylyltransferase in the Synthesis of Uridine 5′‐Diphosphate Galactose and its Application in the Synthesis of N‐Acetyllactosamine

In this study, we describe the direct synthesis of uridine 5′‐diphosphate galactose (UDP‐Gal) by a wild‐type bacterial thymidylyltransferase (RmlA), which is used to synthesize thymidine 5′‐diphosphate glucose (TDP‐glucose) in nature. By using magnesium (Mg²⁺) as a cofactor and a reaction temperature of 55 °C, a one hundred milligram‐scale synthesis of UDP‐Gal was achieved by RmlA. In addition, RmlA was site‐specifically and covalently immobilized on magnetic nanoparticles (MNPa) The resulting RmlA‐MNP complex retained almost 95% of its activity after reuse in ten consecutive enzyme assays. Furthermore, β‐1,4‐galactosyltransferase (GalT) from Neisseria meningitides was successfully overexpressed and purified by using an intein‐mediated protein expression system. GalT was relatively stable at 25 °C, and its activity was enhanced in the presence of DTT and BSA. Thus, it was feasible to synthesize N‐acetyllactosamine (LacNAc) using RmlA and GalT in a sequential addition of enzyme and adjustment of thereaction temperature. These results demonstrate the potential applications of bacterial RmlA in carbohydrate synthesis.