Functional Chromatography Reveals Three Natural Products that Target the Same Protein with Distinct Mechanisms of Action

Access to lead compounds with defined molecular targets continues to be a barrier to the translation of natural product resources. As a solution, we developed a system that uses discrete, recombinant proteins as the vehicles for natural product isolation. Here, we describe the use of this functional chromatographic method to identify natural products that bind to the AAA+ chaperone, p97, a promising cancer target. Application of this method to a panel of fungal and plant extracts identified rheoemodin, 1‐hydroxydehydroherbarin, and phomapyrrolidone A as distinct p97 modulators. Excitingly, each of these molecules displayed a unique mechanism of p97 modulation. This discovery provides strong support for the application of functional chromatography to the discovery of protein modulators that would likely escape traditional high‐throughput or phenotypic screening platforms.     

筛余砂浆气孔结构对其28d抗压强度的影响

为便于高性能混凝土引气剂的开发和应用,采用定量体视学图像分析法测定掺有11种引气剂的混凝土筛余砂浆气孔结构参数,运用灰色关联分析方法研究不同范围孔径对筛余砂浆28d抗压强度的影响,并采用显微硬度仪对筛余砂浆界面过渡区显微硬度进行了测试分析.结果表明：筛余砂浆28d抗压强度与各范围孔径的灰色关联度均为负值,但不同范围孔径对筛余砂浆28d抗压强度的影响有所不同.在筛余砂浆总孔隙率相近条件下,增加10~200μm孔径的孔隙率,降低200~1600μm孔径的孔隙率,可使砂浆气孔的平均孔径减小,砂浆界面过渡区宽度缩短、显微硬度提高,最终减小因引气而造成的砂浆28d抗压强度损失.     

水泥土连拱抗滑墙加固软基边坡的应用研究

软基边坡处理中常采用水泥土搅拌桩复合地基。但受水平推力作用时桩体存在弯折效应且施工时易出现劣质层。本文采用水泥土连拱抗滑墙加固软基边坡,采用数值分析的方法建立三维模型,分析加固前后边坡的水平侧移、沉降、应力、边坡稳定性的变化,并深入的分析了连拱抗滑墙抗滑机理。研究表明连拱抗滑墙加固边坡技术具有性能可靠、施工方便的优势,在软基边坡加固领域具有广阔的应用前景。     

On the Diversity Order of a General Cooperative Relaying Communication System

Cooperative communication technology exploiting distributed spatial diversity is a promising solution for the future high data-rate cellular and ad-hoc wireless communications. To characterize the performance of a cooperative network, the cooperative diversity is commonly used. In this paper, we revisit the concept of diversity and look into the essence of cooperative diversity. We analyze the diversity performance of a general cooperative relaying system with multiple branches. Moreover, each branch consists of one or more hops. Unlike in other analyses, the assumptions that each node being only equipped with a single antenna and that all channel characteristics belonging to the same family are NOT required in our study. Using the decode-and-forward (DF) relaying protocol as an illustration, we provide analytical results on the diversity gain and the coding gain. The results also encompass most of the existing results as special cases. We further show the relationship between the diversity order of a branch and those of the constituent links; and the relationship between the diversity order of the network and those of the constituent branches. In particular, we show that for the uncoded DF protocol, the full diversity order can be achieved by using simple hard-decision detection at the destination. The requirement is to set appropriate signal-to-noise-ratio (SNR) thresholds for the multi-hop branches. To improve the bandwidth efficiency, we also randomly select one branch, among those branches satisfying the SNR-threshold requirement, for the transmission. We show that such a scheme accomplishes the full diversity order and produces a good error performance.     

改进水热法合成ZnO的形貌调控及光学性质

以Zn(NO3)2·6H2O为初始原料,以乙二醇甲醚作溶剂,乙醇胺为沉淀剂,采用水热法制备了不同形貌的ZnO粉体。通过X射线衍射、扫描电镜、荧光光谱等测试手段对不同形貌的ZnO粒子进行了表征。SEM结果表明调节反应物浓度和反应温度能够控制ZnO的尺寸和形貌。荧光光谱测试表明ZnO的紫外发射峰位为399nm,随反应温度升高而发生宽化和红移,而绿光发射峰在453~493nm之间分裂为4个次级峰。     

Effects of Mn2+ on the electrical resistance of electrolessly plated Ni–P thin-film and its application as embedded resistor

The effects of manganese sulfate monohydrate (denoted as MSM) on the properties of electroless Ni–P thin-film were investigated. The properties of Ni–P thin-film were examined by means of atomic force microscopy (AFM), cyclic voltammetry, scanning electron microscopy (SEM), X-ray diffraction, energy-dispersive X-ray spectrometer and X-ray photoelectron spectroscopy. AFM and SEM results showed that MSM had significant influences on the morphology, including the formation of smaller nodules, nano-size particles boundaries on both surface and inside of nodules. Due to the specific topologies, the resistance of Ni–P thin-film deposited with MSM was increased. Based on the characterization results, we summarized that the Mn²⁺ or Mn²⁺-compound were primarily confirmed to act as the stabilizer in Ni–P electroless process. Moreover, the verification test for Ni–P thin-film in application as embedded resistor was carried out through electrical, thermal stability examination by means of current–voltage (I–V), temperature coefficient of resistance and thermal shock tests, respectively. Experiment results demonstrated the Ni–P thin-film deposited with MSM is qualified for embedded resistors.     

Fabrication and polarization-modulated resistive switching behavior of predominantly (110)-oriented BiFeO3 thin films on indium tin oxide/glass substrates

Predominantly (110)-oriented BiFeO₃ (BFO) thin films were deposited on indium tin oxide/glass substrates using a metal organic decomposition process by controlling the preheating temperature. The structure evolution with film thickness at different preheating temperatures was investigated to clarify the growth mode of (110)-predominant BFO film. The formation of the (110)-oriented BFO film is due to the low-temperature nucleation of (110)-oriented grains preheated at 425 °C. In the Au/BFO(110)/ITO heterostructure, a polarization-modulated bistable resistive switching behavior with high ratio of resistance and large diode current characteristics was observed, which makes the heterostructure attractive for application in resistive ferroelectric memory.     

多尺度岩体结构面抗剪强度直剪试验一体机的分级和验证

岩体结构面抗剪强度尺寸效应变化规律的研究需要在专门的多尺度岩体结构面抗剪强度直剪试验机上进行,以获得稳定的抗剪强度力学参数。为保证测试数据的可靠性,在满足测试载荷范围为2～1000 kN时要求恒定荷载示值精度为1％。由于现有测试系统的技术限制,无法实现在同一台设备上进行全部荷载的试验。为此,首先在提出一种设备荷载分级方法的基础上开发了一种多尺度岩体结构面抗剪强度直剪试验一体机,在满足测试精度的要求下分3级尺度直剪仪进行直剪测试,每一级尺度直剪仪设置了荷载重叠区以保证测试数据的等效性。最后对该直剪试验一体机的精度和载荷范围进行了测试,通过了多尺度试样的测试要求。目前该多尺度岩体结构面抗剪直剪试验一体机为多尺度试样的测试研究提供了平台。