Structural Studies of DNA–Cationic Lipid Complexes Confined in Lithographically Patterned Microchannel Arrays

We have used lithographically patterned microchannel arrays with channel widths ranging from 1 to 20 m, fabricated using electron beam lithography and reactive ion etching, in structural studies of DNA–cationic lipid complexes in confinement. Various techniques have been developed for loading these DNA–membrane complexes into the microchannels or to form the complexes in situ by sequentially depositing DNA and lipid solutions into the microchannels. Optical microscopy studies indicate that such complex formation is strongly influenced by the periodic channel structure even at channel widths much larger than the persistent length of the DNA molecules. Preliminary x-ray diffraction experiments conducted at Stanford Synchrotron Radiation Laboratory (SSRL) yielded only a weak signal from the lipid bilayers in the complexes. The use of a microfocused x-ray beam produced by the newly developed Bragg–Fresnel optics at a third-generation synchrotron facility may dramatically increase the signal-to-noise ratio and allow observation of orientational as well as positional ordering of DNA molecules induced by the microchannels. Structural control of the DNA–membrane complexes has a broad range of potential applications in gene probe technology and as mesoscopic biomolecular composites.     

A network-based mobility management scheme for future Internet

The current Internet was originally designed for “fixed” terminals and can hardly support mobility. It is necessary to develop new mobility management schemes for the future Internet. This paper proposes an Identifiers Separating and Mapping Scheme (ISMS), which is a candidate for the future Internet mobility management, and discusses the basic principles and detailed message flow. ISMS is a network-based mobility management scheme that takes advantage of the identity and location separation. The mobility entities in the core network are responsible for the location management. ISMS is designed to satisfy the requirements of faster handover, route optimism, advanced management, location privacy and security. The average handover delay of ISMS is on the order of milliseconds only, which is far smaller than that of Mobile IPv6. Analyses show that ISMS can reduce packet overhead on wireless channels. We build a prototype and perform some experiments. Results verify the feasibility of ISMS.     

Organizational impact of system quality,information quality,and service quality

Increased organizational dependence on information systems drives management attention towards improving information systems’ quality. A recent survey shows that “Improve IT quality” is one of the top concerns facing IT executives. As IT quality is a multidimensional measure, it is important to determine what aspects of IT quality are critical to organizations to help Chief Information Officers (CIOs) to devise effective IT quality improvement strategies. In this research, we model the relationship between information systems’ (IS) quality and organizational impact. We hypothesize greater organizational impact in situations in which system quality, information quality and service quality are high. We also hypothesize a positive relationship between system quality and information quality. We test our hypotheses using survey data. Our structural equation model exhibits a good fit with the observed data. Our results show that IS service quality is the most influential variable in this model (followed by information quality and system quality), thus highlighting the importance of IS service quality for organizational performance. This paper contributes theoretically to IS success models through the system quality-to-information quality and IS quality-to-organizational impact links. Implications of our results for practice and research are discussed.     

Assessment of working conditions in two different semiconductor manufacturing lines: Effective ergonomics interventions

This article examines two manufacturing lines producing semiconductors using different technology concepts, namely Conventional Line (CL) and Lean Production Line (LPL). Both lines manufacturing the same products were compared using various factors, including working conditions, task risks and dangers of the job, and physical body stress. Ergonomic approaches were adopted in the investigation of the two lines. Survey questionnaires were administered to 30% of the workers, and multiple statistical tests were used to determine crucial predictors and to investigate the interactions between the factors. This research has shown that improved ergonomics factors will lead to better working conditions and thus increased job satisfaction. The observed ergonomic differences of these two production lines are compared, and appropriate managerial remedial action is recommended. The interventions to both the lines should reduce accident rates, minimize waste of resources, improve work effectiveness, and provide a better working environment that enhances employees' morale and maximizes productivity and profits. © 2010 Wiley Periodicals, Inc.     

A comparative study of two techniques for improving power‐handling capability of CMOS T/R switches

This article proposes an asymmetric topology for transmit/receive (T/R) switches and more importantly presents a comparative study of both LC‐tuned and resistive body‐floating techniques for improving the power‐handling capability of the T/R switches in the same 0.18‐μm triple‐well CMOS. It is shown from simulations and measurements that the switches adopting either technique achieve comparable performances. For instance, the switch employing the LC‐tuned body‐floating technique exhibits insertion loss of 1.5 dB, isolation of 23.5 dB, and power‐handling capability of 22.5 dBm at 5.2 GHz, whereas the switch using the resistive body‐floating technique exhibits insertion loss of 1.3 dB, isolation of 24 dB, and power‐handling capability of 22.2 dBm, respectively. Therefore, one can conclude that the asymmetric topology with the resistive body‐floating technique is more suitable for designing T/R switches for wireless local area network applications as it consumes smaller silicon area. © 2010 Wiley Periodicals, Inc. Int J RF and Microwave CAE, 2010.     

Fabrication of metallic micromirror using electroplating technology

A nickel micromirror array was designed and successfully fabricated using a thick photoresist as a sacrificial layer and as a mold for nickel electroplating. It was composed of two address electrodes, two support posts and a nickel mirror plate. The mirror plate, which is supported by two nickel posts, is overhung about 10 μm from the silicon substrate. The nickel mirror plate is actuated by an electrostatic force generated by electrostatic potential difference applied between the mirror plate and the address electrode. Optimized fabrication processes have been developed to reduce residual stress in mirror plate and prevent contact between the mirror plate and the substrate, which ensure a reasonable flat and smooth micromirror for operation at low actuation voltage.

     

Development of a web-based insulin decision aid for the elderly: usability barriers and guidelines

Universal Access in the Information Society - In recent years, researchers have attempted to shift patient decision aids (PDAs) from paper-based to web-based to increase its accessibility. Insulin...     

Comparison of image reconstruction by using near‐field and far‐field data for an imperfect conductor

Image reconstruction by using near‐field and far‐field data for an imperfectly conducting cylinder is investigated. A conducting cylinder of unknown shape and conductivity scatters the incident wave in free space and the scattered near and far fields are measured. By using measured fields, the imaging problem is reformulated into an optimization problem and solved by the genetic algorithm. Numerical results show that the convergence speed and final reconstructed results by using near‐field data are better than those obtained by using far‐field data. This work provides both comparative and quantitative information. © 2001 John Wiley & Sons, Inc. Int J RF and Microwave CAE 11: 69–73, 2001.     

物流配送车辆计划调度系统的设计与实现

深入探讨了物流配送系统信息支撑台的要求与结构,详细介绍了作者开发的财政物流配送车辆计划调度系统的设计和实现过程,最后介绍了该计划调度系统的主要功能。