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131.
We describe the application of knowledge-based potentials implemented in the MOE program to compare the ligand-binding sites of several proteins. The binding probabilities for a polar and a hydrophobic probe are calculated on a grid to allow easy comparison of binding sites of superimposed related proteins. The method is fast and simple enough to simultaneously use structural information of multiple proteins of a target family. The method can be used to rapidly cluster proteins into subfamilies according to the similarity of hydrophobic and polar fields of their ligand-binding sites. Regions of the binding site which are common within a protein family can be identified and analysed for the design of family-targeted libraries or those which differ for improvement of ligand selectivity. The field-based hierarchical clustering is demonstrated for three protein families: the ligand-binding domains of nuclear receptors, the ATP-binding sites of protein kinases and the substrate binding sites of proteases. More detailed comparisons are presented for serine proteases of the chymotrypsin family, for the peroxisome proliferator-activated receptor subfamily of nuclear receptors and for progesterone and androgen receptor. The results are in good accordance with structure-based analysis and highlight important differences of the binding sites, which have been also described in the literature. 相似文献
132.
Hans Christian Boas 《Language Resources and Evaluation》2006,40(2):153-174
New methods of documenting languages with digital technologies has led to a multitude of different formats that are difficult
to reuse over time. To overcome the problems surrounding the portability of digital language documentation, linguists are
in the process of formulating best-practice recommendations to increase the likelihood of their work's long-term survival.
This paper describes the implementation of a comprehensive set of current best-practice recommendations pertaining to content,
format, discovery, access, citation, preservation, and rights in the context of the language documentation efforts of the
Texas German Dialect Project. This project is different from others in that it is not primarily concerned with digitizing
and archiving existing recordings. Instead, the archive it is creating is the end-result of a research project whose workflow
begins with data-collection in the filed and ends with depositing digitized and annotated language materials in a web-accessible
digital archive of Texas German. This paper shows how a number of conflicting best-practice recommendations can be resolved,
thereby satisfying the diverse needs of academic research, teaching, and outreach to the community. As such, the results reported
here are an important contribution to the search for strategies guaranteeing the long-term survival of digital language documentation
resources.
相似文献
Hans Christian BoasEmail: |
133.
Coordination components for collaborative virtual environments 总被引:1,自引:0,他引:1
Alberto B. Raposo Adailton J. A. da Cruz Christian M. Adriano Lo P. Magalhes 《Computers & Graphics》2001,25(6):1025-1039
This paper deals with the behavior of virtual environments from the collaboration point-of-view, in which actors (human or virtual beings) interact and collaborate by means of interdependent tasks. In this sense, actors may realize tasks that are dependent on tasks performed by other actors, while the interdependencies between tasks (through resource management and temporal relations) delineate the overall behavior of a virtual environment. Our main goal is to propose an approach for the coordination of those behaviors. Initially a generic study of possible interdependencies between collaborative tasks is presented, followed by the formal modeling (using Petri Nets) of coordination mechanisms for those dependencies. In order to implement such mechanisms, an architecture of reusable and pluggable coordination components is also introduced. These components are used in an implementation of a multi-user videogame. The presented approach is a concrete step to create virtual societies of actors that collaborate to reach common goals without the risk of getting involved in conflicting or repetitive tasks. 相似文献
134.
Critical success factors revisited: success and failure cases of information systems for senior executives 总被引:1,自引:0,他引:1
The literature suggests the existence of critical success factors (CSFs) for the development of information systems that support senior executives. Our study of six organizations gives evidence for this notion of CSFs. The study further shows an interesting pattern, namely that companies either “get it right”, and essentially succeed on all CSFs, or “get it completely wrong”, that is, fall short on each of the CSFs. Among the six cases for which data were collected through in-depth interviews with company executives, three organizations seemed to manage all the CSFs properly, while two others managed all CSFs poorly. Only one organization showed a mixed scorecard, managing some factors well and some not so well. At the completion of the study, this organization could neither be judged as a success, nor as a failure. This dichotomy between success and failure cases suggests the existence of an even smaller set of “meta-success” factors. Based on our findings, we speculate that these “meta-success” factors are “championship”, “availability of resources”, and “link to organization objectives”. 相似文献
135.
The use of information technology to enhance teaching and learning processes has been practiced for a number of years now.
However, the rapid growth in the use of the Internet has led to a new dimension in interactive and collaborative learning
anytime and anyplace dynamically. With the explosion of “virtual education initiatives”, the question of the feasibility and
success criteria for such projects quickly arises. To address the question in an organized way, we propose a project assessment
based on critical success factors. Hence, in this article, we draw on a widely recognized critical success factor framework.
We (slightly) adjust the framework to fit the special characteristics of virtual education initiatives, and apply to one case
study, namely the virtual education initiative at the Faculty of Business at the City University of Hong Kong. The results
suggest that the past success of the case is due to the adherence to the large majority of critical success factors. However,
it also outlines some areas of concern. The paper concludes with a discussion on the strength and limitations of virtual learning
environment as well as future directions.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
136.
This paper outlines a generic evaluation methodology for multimedia and real time applications. It concentrates on the application layer and the service aspect. Principles of software evaluation for quality assessment and measurement for networked services and distributed applications are used to present a specific method for measuring important characteristics. The method and the measurement procedure allow comparison of the actual characteristics of service quality with the required characteristics and thresholds. The proof of concepts will be made by the implementation of quality measurement agents following the approach outlined in this paper. Our approach of performing service level management (SLM) with agent technology is distributed, slim, minimizable to the maximum, independent in its methodology and offers comparable, objective results. 相似文献
137.
Ulrich Schoch Jürg Lüthy und Christian Schlatter 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1984,178(5):351-355
Zusammenfassung In Rohextrakten von 18Penicillium camemberti- und 6P. roqueforti-Stämmen, die als Reifungsorganismen bei der Schimmelkäseproduktion in Schweizer Käsereien eingesetzt werden, wurde das mutagene Potential mit Hilfe des Ames-Testes bestimmt. In keinem der geprüften Extrakte konnte eine mutagene Aktivität festgestellt werden. Roquefortin, ein in Blauschimmelkäsen nachweisbares Mykotoxin vonP. roqueforti, erwies sich im Ames-Test als nicht mutagen. Die ermittelten Resultate ergaben keine Anhaltspunkte für genotoxische Effekte durch die Konsumation von schimmelgereiftem Käse.
Auszug aus der Dissertation von U. Schoch, Mykotoxine in schimmelgereiften Käsen, Diss. ETH Nr. 7278, ETH Zürich, 1983 相似文献
Mutagenicity testing of commercialP. Camemberti- andP. roqueforti-strains
Summary We tested the mutagenic potential of crude extracts of 18 strains ofPenicillium camemberti- and 6 strains ofP. roqueforti, which are used commercially in the production of mould ripened cheese in Switzerland. No mutagenic activity could be detected in any of the extracts. Roquefortine, a mycotoxin ofP. roqueforti, often found in Blue cheese, was negative in the Amestest. The results obtained do not lead reasons for experting undesired long term effects from the consumption of mould ripened cheese.
Auszug aus der Dissertation von U. Schoch, Mykotoxine in schimmelgereiften Käsen, Diss. ETH Nr. 7278, ETH Zürich, 1983 相似文献
138.
Viet-Phuong Nguyen Christian Prins Caroline Prodhon 《Engineering Applications of Artificial Intelligence》2012,25(1):56-71
The two-echelon location-routing problem (LRP-2E) is raised by the design of transportation networks with two types of trips: first-level trips serving from one main depot a set of satellite depots, to be located, and second-level trips supplying customers from these satellites. In the proposed multi-start iterated local search (MS-ILS), three greedy randomized heuristics are used cyclically to get initial solutions. Each ILS run alternates between two search spaces: LRP-2E solutions, and travelling salesman (TSP) tours covering the main depot and the customers. The number of iterations allotted to a run is reduced whenever a known solution (stored in a tabu list) is revisited. MS-ILS can be reinforced by a path-relinking procedure (PR), used internally for intensification, as post-optimization, or both. On two sets with 24 and 30 LRP-2E instances, MS-ILS outperforms on average two GRASP algorithms and adding PR brings a further improvement. Our metaheuristic also surpasses a tabu search on 30 instances for a more general problem with several main depots. It is still effective on a particular case, the capacitated location-routing problem (CLRP): In a comparison with four published metaheuristics, only one (LRGTS, Prins et al., 2007) does better. 相似文献
139.
Christian Bur Peter Reimann Mike Andersson Anita Lloyd Spetz Andreas Schütze 《Microsystem Technologies》2012,18(7-8):1015-1025
A silicon carbide based enhancement type metal insulator field effect transistor with porous gate metallization has been investigated as a total NO x sensor operated in a temperature cycling mode. This operating mode is quite new for gas sensors based on the field effect but promising results have been reported earlier. Based on static investigations we have developed a suitable T-cycle optimized for NO x detection and quantification in a mixture of typical exhaust gases (CO, C2H4, and NH3). Significant features describing the shape of the sensor response have been extracted and evaluated with multivariate statistics (e.g. linear discriminant analysis) allowing quantification of NO x . Additional cleaning-cycles every 30?min improve the stability of the sensor further. With this kind of advanced signal processing the influence of sensor drift and cross sensitivity to ambient gases can be reduced effectively. Measurements have proven that different concentrations of NO x can be detected even in a changing mixture of other typical exhaust gases under dry and humid conditions. In addition to that, unknown concentrations of NO x can be detected based on a small set of training data. It can be concluded that the performance of GasFETs for NO x determination can be enhanced considerably with temperature cycling and appropriate signal processing. 相似文献
140.
Forecasting and trading the EUR/USD exchange rate with Gene Expression and Psi Sigma Neural Networks
Georgios Sermpinis Jason Laws Andreas Karathanasopoulos Christian L. Dunis 《Expert systems with applications》2012,39(10):8865-8877
The motivation for this paper is to investigate the use of two promising classes of artificial intelligence models, the Psi Sigma Neural Network (PSI) and the Gene Expression algorithm (GEP), when applied to the task of forecasting and trading the EUR/USD exchange rate. This is done by benchmarking their results with a Multi-Layer Perceptron (MLP), a Recurrent Neural Network (RNN), a genetic programming algorithm (GP), an autoregressive moving average model (ARMA) plus a naïve strategy. We also examine if the introduction of a time-varying leverage strategy can improve the trading performance of our models. 相似文献