Measurement of oocyte temporal maturation process by means of a simple optical micro-system

We present a simple optical micro-system used to measure the transmission spectra of oocytes in order to qualify their maturation stage. Two applications of the device are possible: (i) the evaluation of the maturation stages of oocytes, and (ii) the development of a fertilization indicator. For the first application, GV, MI and MII oocytes were also analysed. Transmission spectra allow the 3 maturation stages to be identified but cannot be used to estimate the maturity of an unknown oocyte. Oocytes are subject to continuous development. This is why spectral separation of the 3 maturation stages cannot be made although they can be visually identified. However, the visual observation remains biologist-dependent. We therefore investigated the temporal maturation evolution of the oocytes in terms of transmission spectra and probability analysis. Results show that oocytes to be fertilized should not only be chosen in the MII stage, but also at the right time during the MII stage. This particular aspect requires further investigation. However, spectral measurements could be used as a technique for monitoring the maturation evolution of the oocytes. Fertilized oocytes exhibiting fertilization abnormalities were also tested. The device proved to be an efficient fertilization indicator.     

An Efficient Newton-Krylov Implementation of the Constrained Runs Scheme for Initializing on a Slow Manifold

The long-term dynamic behavior of many dynamical systems evolves on a low-dimensional, attracting, invariant slow manifold, which can be parameterized by only a few variables (“observables”). The explicit derivation of such a slow manifold (and thus, the reduction of the long-term system dynamics) is often extremely difficult or practically impossible. For this class of problems, the equation-free framework has been developed to enable performing coarse-grained computations, based on short full model simulations. Each full model simulation should be initialized so that the full model state is consistent with the values of the observables and close to the slow manifold. To compute such an initial full model state, a class of constrained runs functional iterations was proposed (Gear and Kevrekidis, J. Sci. Comput. 25(1), 17–28, 2005; Gear et al., SIAM J. Appl. Dyn. Syst. 4(3), 711–732, 2005). The schemes in this class only use the full model simulator and converge, under certain conditions, to an approximation of the desired state on the slow manifold. In this article, we develop an implementation of the constrained runs scheme that is based on a (preconditioned) Newton-Krylov method rather than on a simple functional iteration. The functional iteration and the Newton-Krylov method are compared in detail using a lattice Boltzmann model for one-dimensional reaction-diffusion as the full model simulator. Depending on the parameters of the lattice Boltzmann model, the functional iteration may converge slowly or even diverge. We show that both issues are largely resolved by using the Newton-Krylov method, especially when a coarse grid correction preconditioner is incorporated.     

Using coverage to automate and improve test purpose based testing

Test purposes have been presented as a solution to avoid the state space explosion when selecting test cases from formal models. Although such techniques work very well with regard to the speed of the test derivation, they leave the tester with one important task that influences the quality of the overall testing process: test purposes have to be formulated manually. In this paper, we present an approach that assists a test engineer with test purpose design in two ways: it allows automatic generation of coverage based test suites and can be used to automatically exercise those aspects of the system that are missed by hand-crafted test purposes. We consider coverage of Lotos specifications, and show how labeled transition systems derived from such specifications have to be extended in order to allow the application of logical coverage criteria to Lotos specifications. We then show how existing tools can be used to efficiently derive test cases and suggest how to use the coverage information to minimize test suites while generating them.     

The impact of false warnings on partial and full lane departure warnings effectiveness and acceptance in car driving

In the past, lane departure warnings (LDWs) were demonstrated to improve driving behaviours during lane departures but little is known about the effects of unreliable warnings. This experiment focused on the influence of false warnings alone or in combination with missed warnings and warning onset on assistance effectiveness and acceptance. Two assistance unreliability levels (33 and 17%) and two warning onsets (partial and full lane departure) were manipulated in order to investigate interaction. Results showed that assistance, regardless unreliability levels and warning onsets, improved driving behaviours during lane departure episodes and outside of these episodes by favouring better lane-keeping performances. Full lane departure and highly unreliable warnings, however, reduced assistance efficiency. Drivers’ assistance acceptance was better for the most reliable warnings and for the subsequent warnings. The data indicate that imperfect LDWs (false warnings or false and missed warnings) further improve driving behaviours compared to no assistance.

Practitioner Summary: This study revealed that imperfect lane departure warnings are able to significantly improve driving performances and that warning onset is a key element for assistance effectiveness and acceptance. The conclusion may be of particular interest for lane departure warning designers.     

Supervised image segmentation using Q-Shift Dual-Tree Complex Wavelet Transform coefficients with a texton approach

In this study, we propose a simple and efficient texture-based algorithm for image segmentation. This method constitutes computing textons and bag of words (BOWs) learned by support vector machine (SVM) classifiers. Textons are composed of local magnitude coefficients that arise from the Q-Shift Dual-Tree Complex Wavelet Transform (DT-CWT) combined with color components. In keeping with the needs of our research context, which addresses land cover mapping from remote images, we use a few small texture patches at the training stage, where other supervised methods usually train fully representative textures. We accounted for the scale and rotation invariance issue of the textons, and three different invariance transforms were evaluated on DT-CWT-based features. The largest contribution of this study is the comparison of three classification schemes in the segmentation algorithm. Specifically, we designed a new scheme that was especially competitive and that uses several classifiers, with each classifier adapted to a specific size of analysis window in texton quantification and trained on a reduced data set by random selection. This configuration allows quick SVM convergence and an easy parallelization of the SVM-bank while maintaining a high segmentation accuracy. We compare classification results with textons made using the well-known maximum response filters bank and speed up robust features features as references. We show that DT-CWT textons provide better distinguishing features in the entire set of configurations tested. Benchmarks of our different method configurations were made over two substantial textured mosaic sets, each composed of 100 grey or color mosaics made up of Brodatz or VisTex textures. Lastly, when applied to remote sensing images, our method yields good region segmentation compared to the ENVI commercial software, which demonstrates that the method could be used to generate land cover maps and is suitable for various purposes in image segmentation.     

SEBS elastomers for fabrication of microfluidic devices with reduced drug absorption by injection molding and extrusion

The majority of microfluidic devices used for cell culture, including Organ-on-a-Chips (Organ Chips), are fabricated using polydimethylsiloxane (PDMS) polymer because it is flexible, optically clear, and easy to mold. However, PDMS possesses significant challenges for high volume manufacturing and its tendency to absorb small hydrophobic compounds limits its usefulness as a material in devices used for drug evaluation studies. Here, we demonstrate that a subset of optically clear, elastomeric, styrenic block copolymers based on styrene-ethylene-butylene-styrene exhibit reduced absorption of small hydrophobic molecules and drug compounds compared to PDMS and that they can be fabricated into microfluidic devices with fine features and the flexibility required for Organ Chips using mass production techniques of injection molding and extrusion.     

Simultaneous automated design of structured QFT controller and prefilter using nonlinear programming

This paper describes a nonlinear programming‐based robust design methodology for controllers and prefilters of a predefined structure for the linear time‐invariant systems involved in the quantitative feedback theory. This controller and prefilter synthesis problem is formulated as a single optimization problem with a given performance optimization objective and constraints enforcing stability and various specifications usually enforced in the quantitative feedback theory. The focus is set on providing constraints expression that can be used in standard nonlinear programming solvers. The nonlinear solver then computes in a single‐step controller and prefilter design parameters that satisfy the prescribed constraints and maximizes the performance optimization objective. The effectiveness of the proposed approach is demonstrated through a variety of difficult design cases like resonant plants, open‐loop unstable plants, and plants with variation in the time delay. Copyright © 2016 John Wiley & Sons, Ltd.     

Compliance by design – Bridging the chasm between auditors and IT architects

System and process auditors assure – from an information processing perspective – the correctness and integrity of the data that is aggregated in a company’s financial statements. To do so, they assess whether a company’s business processes and information systems process financial data correctly. The audit process is a complex endeavor that in practice has to rely on simplifying assumptions. These simplifying assumptions mainly result from the need to restrict the audit scope and to focus it on the major risks. This article describes a generalized audit process. According to our experience with this process, there is a risk that material deficiencies remain undiscovered when said simplifying assumptions are not satisfied. To address this risk of deficiencies, the article compiles thirteen control patterns, which – according to our experience – are particularly suited to help information systems satisfy the simplifying assumptions. As such, use of these proven control patterns makes information systems easier to audit and IT architects can use them to build systems that meet audit requirements by design. Additionally, the practices and advice offered in this interdisciplinary article help bridge the gap between the architects and auditors of information systems and show either role how to benefit from an understanding of the other role’s terminology, techniques, and general work approach.