Progression and Dissemination of Pulmonary Mycobacterium Avium Infection in a Susceptible Immunocompetent Mouse Model

Pulmonary infections caused by the group of nontuberculosis mycobacteria (NTM), Mycobacterium avium complex (MAC), are a growing public health concern with incidence and mortality steadily increasing globally. Granulomatous inflammation is the hallmark of MAC lung infection, yet reliable correlates of disease progression, susceptibility, and resolution are poorly defined. Unlike widely used inbred mouse strains, mice that carry the mutant allele at the genetic locus sst1 develop human-like pulmonary tuberculosis featuring well-organized caseating granulomas. We characterized pulmonary temporospatial outcomes of intranasal and left intrabronchial M. avium spp. hominissuis (M.av) induced pneumonia in B6.Sst1S mice, which carries the sst1 mutant allele. We utilized traditional semi-quantitative histomorphological evaluation, in combination with fluorescent multiplex immunohistochemistry (fmIHC), whole slide imaging, and quantitative digital image analysis. Followingintrabronchiolar infection with the laboratory M.av strain 101, the B6.Sst1S pulmonary lesions progressed 12–16 weeks post infection (wpi), with plateauing and/or resolving disease by 21 wpi. Caseating granulomas were not observed during the study. Disease progression from 12–16 wpi was associated with increased acid-fast bacilli, area of secondary granulomatous pneumonia lesions, and Arg1+ and double positive iNOS+/Arg1+ macrophages. Compared to B6 WT, at 16 wpi, B6.Sst1S lungs exhibited an increased area of acid-fast bacilli, larger secondary lesions with greater Arg1+ and double positive iNOS+/Arg1+ macrophages, and reduced T cell density. This morphomolecular analysis of histologic correlates of disease progression in B6.Sst1S could serve as a platform for assessment of medical countermeasures against NTM infection.     

Molecular Effects of Low-Intensity Shock Wave Therapy on L6 Dorsal Root Ganglion/Spinal Cord and Blood Oxygenation Level-Dependent (BOLD) Functional Magnetic Resonance Imaging (fMRI) Changes in Capsaicin-Induced Prostatitis Rat Models

Neurogenic inflammation and central sensitization play a role in chronic prostatitis/chronic pelvic pain syndrome. We explore the molecular effects of low-intensity shock wave therapy (Li-ESWT) on central sensitization in a capsaicin-induced prostatitis rat model. Male Sprague–Dawley rats underwent intraprostatic capsaicin (10 mM, 0.1 cm³) injections. After injection, the prostate received Li-ESWT twice, one day apart. The L6 dorsal root ganglion (DRG)/spinal cord was harvested for histology and Western blotting on days 3 and 7. The brain blood oxygenation level-dependent (BOLD) functional images were evaluated using 9.4 T fMRI before the Li-ESWT and one day after. Intraprostatic capsaicin injection induced increased NGF-, BDNF-, and COX-2-positive neurons in the L6 DRG and increased COX-2, NGF, BDNF, receptor Trk-A, and TRPV1 protein expression in the L6 DRG and the dorsal horn of the L6 spinal cord, whose effects were significantly downregulated after Li-ESWT on the prostate. Intraprostatic capsaicin injection increased activity of BOLD fMRI responses in brain regions associated with pain-related responses, such as the caudate putamen, periaqueductal gray, and thalamus, whose BOLD signals were reduced after Li-ESWT. These findings suggest a potential mechanism of Li-ESWT on modulation of peripheral and central sensitization for treating CP/CPPS.     

Topology-aided cross-layer fast handoff designs for IEEE 802.11/mobile IP environments

This study first reviews state-of-the-art fast handoff techniques for IEEE 802.11 or Mobile IP networks. Based on that review, topology-aided cross-layer fast handoff designs are proposed for Mobile IP over IEEE 802.1.1 networks. Time-sensitive applications, such as voice over IP (VoIP), cannot tolerate the long layer-2 plus layer-3 handoff delays that arise in IEEE 802.11/Mobile IP environments. Cross-layer designs are increasingly adopted to shorten the handoff latency time. Handoff-related layer-2 triggers may reduce the delay between layer-2 handoff completion and the associated layer-3 handoff activation. Cross-layer topology information, such as the association between 802.11 access points and Mobile IP mobility agents, together with layer-2 triggers, can be utilized by a mobile node to start layer-3 handoff-related activities, such as agent discovery, address configuration, and registration, in parallel with or prior to those of layer-2 handoff. Experimental results indicate that the whole handoff. delay can meet the delay requirement of VoIP applications when layer-3 handoff activities occur prior to layer-2 handoffs.     

A novel dynamic Multiple Ring-based Local Restoration for point-to-multipoint multicast traffic in WDM mesh networks

Optical networks with DWDM (Dense Wavelength Division Multiplex) can provide multiple data channels to supply high speed, high capacity to perform bandwidth-intensive multicast transmission service. Light-tree is a popular technique applied to support point-to-multipoint multicast services. Any failure during a multicast session would cause severe service loss or disruptions, especially when the faults occur near the source node. A novel ring-based local fault recovery mechanism, Multiple Ring-based Local Restoration (MRLR), for point-to-multipoint multicast traffic based on the minimum spanning tree (MST) in WDM mesh networks is proposed in this article. The MRLR mechanism dismembers the multicast tree into several disjoint segment-blocks (sub-trees) and reserves preplanned spare capacity to set up multiple protection rings in each segment-block for providing rapid local recovery. The MRLR scheme outperforms other methodologies in terms of the blocking probability, recovery time, and average hop count of protection path per session for different network topologies.     

Acoustooptic Coherent Mode Coupling in Polarization-Maintaining Fiber and Its Application as a Variable-Polarization-Dependent Loss Element

In this letter, we investigate coherent acoustooptic coherent mode coupling from the LP₀₁ core mode to LP_1m cladding mode of a polarization-maintaining fiber (PMF) induced by two acoustic gratings. We show narrowband variable attenuation along either birefringent axis of the PMF by varying the relative phase between the two acoustic gratings. By launching two acoustic gratings at different frequencies, variable attenuation and low crosstalk along either birefringent axis within the acoustooptic coupling bandwidth are demonstrated     

A lightweight,self‐adaptive lock gate designation scheme for data collection in long‐thin wireless sensor networks

Constrained by the physical environments, the long‐thin topology has recently been promoted for many practical deployments of wireless sensor networks (WSNs). In general, a long‐thin topology is composed of a number of long branches of sensor nodes, where along a branch each sensor node has only one potential parent node toward the sink node. Although data aggregation may alleviate excessive packet contention, the maximum payload size of a packet and the dynamically changing traffic loads may severely affect the amount of sensor readings that may be collected along a long branch of sensor nodes. In addition, many practical applications of long‐thin WSNs demand the exact sensor readings at each location along the deployment areas for monitoring and analysis purposes, so sensor readings may not be aggregated when they are collected. This paper proposes a lightweight, self‐adaptive scheme that designates multiple collection nodes, termed lock gates, along a long‐thin network to collect sensor readings sent from their respective upstream sensor nodes. The self‐adaptive lock gate designation scheme balances between the responsiveness and the congestion of data collection while mitigating the funneling effect. The scheme also dynamically adapts the designation of lock gates to accommodate the time‐varying sensor reading generation rates of different sensor nodes. A testbed of 100 Jennic sensor nodes is developed to demonstrate the effectiveness of the proposed lock gate designation scheme. Copyright © 2011 John Wiley & Sons, Ltd.     

Near optimal routing and capacity management for PWCE-based survivable WDM networks

Protected Working Capacity Envelope (PWCE) has been proposed to simplify resource management and traffic control for survivable WDM networks. In a PWCE-based network, part of the link capacity is reserved for accommodating working routes, and the remaining capacity is reserved for backup routes. The shortest path routing is applied in PWCE-based networks. An arrival call is accepted only when each link along the shortest path has a free working channel. Such a working path routing scheme greatly simplifies the call admission control process for dynamic traffic, and it is especially suitable for implementation in a distributed manner among network nodes. In this article, we investigate two protection strategies: Bundle Protection (BP) and Individual Protection (IDP). In BP, only one backup path can be used to protect a failure component, whereas multiple backup paths can be used in IDP. We formulate four mixed integer non-linear programming (MINLP) problems using BP and IDP strategies for single link and single node failure protection. Each model is designed to determine link metrics for shortest working path routing, working and backup channel assignments, and backup path planning. Our objective is to minimize call-blocking probability on the bottleneck link. Since these models are highly non-linear and non-convex, it is difficult to obtain exact global optimal solutions. We propose a Simulated Annealing-based Heuristic (SAH) algorithm to obtain near optimal solutions. This SAH adopts the concepts of simulated annealing as well as the bi-section technique to minimize call-blocking probabilities. To evaluate the performance, we made simulation comparisons between SAH and the unity link weight assignment scheme. The results indicate that SAH can greatly reduce call-blocking probabilities on benchmark and the randomly generated networks.     

Lateral Nonuniformity Effects of Border Traps on the Characteristics of Metal–Oxide–Semiconductor Field-Effect Transistors Subjected to High-Field Stresses

The lateral nonuniformity (LNU) effects of border traps are studied by exploring both the high- and low-frequency characteristics in N-type channel metal-oxide-semiconductor field-effect transistors. According to experimental data, the deterioration of nonuniformity is significantly enhanced at low frequencies. The cause may be due to the additional trapped charges of border traps (near-interface oxide traps) under the low-frequency measurement. This model is successfully simulated by the combination of low-frequency C-V curves with the heavily and lightly damaged regions. Additionally, the double-peak charge-pumping current is observed in low-frequency measurements, which can further support our hypothesis that border-trap-enhanced LNU exists. Finally, the geometric effect of the polygate and the thickness effect of the gate oxide are also investigated for the nonuniformity issue.     

The effects of boron penetration on p+ polysilicon gatedPMOS devices

The penetration of boron into and through the gate oxides of PMOS devices which employ p⁺ doped polysilicon gates is studied. Boron penetration results in large positive shifts in V_FB , increased PMOS subthreshold slope and electron trapping rate, and decreased low-field mobility and interface trap density. Fluorine-related effects caused by BF₂ implantations into the polysilicon gate are shown to result in PMOS threshold voltage instabilities. Inclusion of a phosphorus co-implant or TiSi₂ salicide prior to gate implantation is shown to minimize this effect. The boron penetration phenomenon is modeled by a very shallow, fully-depleted p-type layer in the silicon substrate close to the SiO ₂/Si interface