Mydriatics release from solid and semi-solid ophthalmic formulations using different in vitro methods

The aim of the present paper was the development of semi-solid (hydrogels) and solid (film) ophthalmic formulations for the controlled release of two mydriatics: phenylephrine and tropicamide. The formulations – based on polyvinylalcohol and hyaluronic acid – were characterized, and release studies were performed with three different in vitro set-ups, i.e. Franz-type diffusion cell, vial method and inclined plane; for comparison, a solution and a commercial insert, both clinically used to induce mydriasis, were evaluated. Both gels and film allowed for a controlled release of drugs, appearing a useful alternative for mydriatics administration. However, the release kinetic was significantly influenced by the method used, highlighting the need for optimization and standardization of in vitro models for the evaluation of drug release from ophthalmic dosage forms.     

Solving advanced multi-objective robust designs by means of multiple objective evolutionary algorithms (MOEA): A reliability application

This paper extends the approach proposed by the second author in [Rocco et al. Robust design using a hybrid-cellular-evolutionary and interval-arithmetic approach: a reliability application. In: Tarantola S, Saltelli A, editors. SAMO 2001: Methodological advances and useful applications of sensitivity analysis. Reliab Eng Syst Saf 2003;79(2):149-59 [special issue]] to obtain a robust system design. The approach based on the use of evolutionary algorithms and interval arithmetic finds the maximum-volume inner box (MIB) or the maximal ranges of variation for each variable that preserve pre-specified design/performance requirements. The original single-objective formulation considers the definition of a MIB around a specified centroid (case 1), or around an unspecified centroid (case 2). In this paper, both cases were successfully modified and solved as multiple-objective (MO) problems, showing the advantages of MO formulations in a design-selection decision framework. Special attention is devoted to the unspecified centre MO problem where the computational efficiency could be a critical issue. In that sense, a new procedure based on the “percentage representation” is proposed. This approach reduces drastically the computational burden, extending the possibilities of use of robust design.     

Porous NiTi shape memory alloys produced by SHS: microstructure and biocompatibility in comparison with Ti2Ni and TiNi3

Shape memory alloys based on NiTi have found their main applications in manufacturing of new biomedical devices mainly in surgery tools, stents and orthopedics. Porous NiTi can exhibit an engineering elastic modulus comparable to that of cortical bone (12–17 GPa). This condition, combined with proper pore size, allows good osteointegration. Open cells porous NiTi was produced by self propagating high temperature synthesis (SHS), starting from Ni and Ti mixed powders. The main NiTi phase is formed during SHS together with other Ni–Ti compounds. The biocompatibility of such material was investigated by single culture experiment and ionic release on small specimen. In particular, NiTi and porous NiTi were evaluated together with elemental Ti and Ni reference metals and the two intermetallic TiNi₃, Ti₂Ni phases. This approach permitted to clearly identify the influence of secondary phases in porous NiTi materials and relation with Ni-ion release. The results indicated, apart the well-known high toxicity of Ni, also toxicity of TiNi₃, whilst phases with higher Ti content showed high biocompatibility. A slightly reduced biocompatibility of porous NiTi was ascribed to combined effect of TiNi₃ presence and topography that requires higher effort for the cells to adapt to the surface.     

Circularly polarized beams and vortex generation in uniaxial media

We deduce the expressions for the two circularly polarized components of a paraxial beam propagating along the optical axis of a uniaxial crystal. We find that each of them is the sum of two contributions, the first being a free field and the second describing the interaction with the opposite component. Moreover, we expand both components as a superposition of vortices of any order, thus obtaining a complete physical picture of the interaction dynamics. Consequently, we argue that a left-hand circularly polarized incoming beam, endowed with a circular symmetric profile, gives rise, inside the crystal, to a right-hand circularly polarized vortex of order 2. The efficiency of this vortex generation is investigated by means of a power exchange analysis. The Gaussian case is fully discussed, showing the relevant features of the vortex generation.     

Application of Brazilian pine-fruit shell as a biosorbent to removal of reactive red 194 textile dye from aqueous solution kinetics and equilibrium study

The Brazilian pine-fruit shell (Araucaria angustifolia) is a food residue, that was used as biosorbent for the removal of non-hydrolyzed reactive red 194 (NRR) and hydrolyzed reactive red 194 (HRR) forms from aqueous solutions. Chemical treatment of Brazilian pine-fruit shell (PW), with chromium (Cr-PW), with acid (A-PW), and with acid followed by chromium (Cr-A-PW) were also tested as alternative biosorbents for the removal of NRR and HRR from aqueous effluents. It was observed that the treatment of the Brazilian pine-fruit shell with chromium (Cr-PW and Cr-A-PW) leaded to a remarkable increase in the specific surface area and average porous volume of these biosorbents when compared to unmodified Brazilian pine-fruit shell (PW). The effects of shaking time, biosorbent dosage and pH on biosorption capacity were studied. In acidic pH region (pH 2.0) the biosorption of NRR and HRR were favorable. The contact time required to obtain the equilibrium was 24h at 25 degrees C. The equilibrium data were fitted to Langmuir, Freundlich, Sips and Redlich-Peterson isotherm models. For NRR reactive dye the equilibrium data were best fitted to the Sips isotherm model using PW and A-PW as biosorbents, and Redlich-Peterson isotherm model using Cr-PW and Cr-A-PW as biosorbents. For HRR reactive dye the equilibrium data were best fitted to the Sips isotherm model using PW, A-PW and Cr-A-PW and the Redlich-Peterson isotherm model for Cr-PW as biosorbent.     

Model building and assessment of the impact of covariates for disease prevalence mapping in low-resource settings: to explain and to predict

This paper provides statistical guidance on the development and application of model-based geostatistical methods for disease prevalence mapping. We illustrate the different stages of the analysis, from exploratory analysis to spatial prediction of prevalence, through a case study on malaria mapping in Tanzania. Throughout the paper, we distinguish between predictive modelling, whose main focus is on maximizing the predictive accuracy of the model, and explanatory modelling, where greater emphasis is placed on understanding the relationships between the health outcome and risk factors. We demonstrate that these two paradigms can result in different modelling choices. We also propose a simple approach for detecting over-fitting based on inspection of the correlation matrix of the estimators of the regression coefficients. To enhance the interpretability of geostatistical models, we introduce the concept of domain effects in order to assist variable selection and model validation. The statistical ideas and principles illustrated here in the specific context of disease prevalence mapping are more widely applicable to any regression model for the analysis of epidemiological outcomes but are particularly relevant to geostatistical models, for which the separation between fixed and random effects can be ambiguous.     

Investments in big data analytics and firm performance: an empirical investigation of direct and mediating effects

Big data has recently been recognised as one of the most important areas of future technology. It has attracted the attention of many industries, since it has the potential to provide companies with high business value. This paper examines the forms of business value that companies can create from big data analytics investments, the direct impacts it has on the financial performance of a firm, and the mediating effects of market performance and customer satisfaction. Drawing on the resource-based view theory, this study demonstrates that the business value achieved from investments in big data analytics leads to advantages in terms of the financial performance of a firm. The results offer evidence of the existence of a customer satisfaction mediation effect and of the absence of a market performance mediation effect. Theoretical and practical implications are discussed at the end of the paper.     

A subcellular model of glucose-stimulated pancreatic insulin secretion

When glucose is raised from a basal to stimulating level, the pancreatic islets respond with a typical biphasic insulin secretion pattern. Moreover, the pancreas is able to recognize the rate of change of the glucose concentration. We present a relatively simple model of insulin secretion from pancreatic beta-cells, yet founded on solid physiological grounds and capable of reproducing a series of secretion patterns from perfused pancreases as well as from stimulated islets. The model includes the notion of distinct pools of granules as well as mechanisms such as mobilization, priming, exocytosis and kiss-and-run. Based on experimental data, we suggest that the individual beta-cells activate at different glucose concentrations. The model reproduces most of the data it was tested against very well, and can therefore serve as a general model of glucose-stimulated insulin secretion. Simulations predict that the effect of an increased frequency of kiss-and-run exocytotic events is a reduction in insulin secretion without modification of the qualitative pattern. Our model also appears to be the first physiology-based one to reproduce the staircase experiment, which underlies 'derivative control', i.e. the pancreatic capacity of measuring the rate of change of the glucose concentration.     

Pulsed optically pumped rubidium clock with high frequency-stability performance

In this paper, we present the performance of a vapor-cell rubidium frequency standard working in the pulsed regime, in which the clock signal is represented by a Ramsey pattern observed on an optically detected laser absorption signal. The main experimental results agree with previously reported theoretical predictions. In particular, we measured a relative frequency stability of σy(τ) - 1.6 × 10(-13)τ-1/2 for integration times, τ, up to 200 s, which represents a record in short-term stability for a vapor-cell clock. We also discuss the most important physical phenomena that contribute to this result.     

FRP strengthening of steel and steel-concrete composite structures: an analytical approach

A recent technique for strengthening steel and steel-concrete composite structures by the use of externally bonded Fiber Reinforced Polymer (FRP) sheets, to increase the flexural capacity of the structural element, is described. Several researches developed FRP strengthening of reinforced concrete and masonry structures, but few experimental studies about steel and steel-concrete composite elements are available. Some examples of guidelines for the design and construction of externally bonded FRP systems for strengthening existing metal structures are available, but the method used to predict the flexural behaviour of FRP strengthened elements is usually based on the hypothesis of elastic behaviour of materials and FRP laminate is mainly considered only under the tensile flange. In this paper, an analytical procedure to predict the flexural behaviour of FRP strengthened steel and steel-concrete composite elements, based on cross-sectional behaviour and taking into account the non-linear behaviour of the materials with any configuration of FRP reinforcement, is given. Analytical predictions are compared with some experimental results available in the literature on the flexural behaviour of FRP strengthened steel and steel-concrete composite elements, showing good agreement of the results, even in the non-linear phase, until failure.