Nonproteolytic Clostridium botulinum toxigenesis in cooked turkey stored under modified atmospheres

The ability of nonproteolytic Clostridium botulinum type B spores to grow and produce toxin in cooked, uncured turkey packaged under modified atmospheres was investigated at refrigeration and mild to moderate abuse temperatures. Cook-in-bag turkey breast was carved into small chunks, surface-inoculated with a mixture of nonproteolytic C. botulinum type B spores, packaged in O2-impermeable bags under two modified atmospheres (100% N2 and 30% CO2:70% N2), and stored at 4, 10, and 15 degrees C. Samples were analyzed for botulinal toxin and indigenous microorganisms, as well as subjected to sensory evaluation, on days 0, 7, 14, 28, 42, and 60. Given sufficient incubation time, nonproteolytic C. botulinum type B grew and produced toxin in all temperature and modified atmosphere treatment combinations. At moderate temperature abuse (15 degrees C), toxin was detected by day 7, independent of packaging atmosphere. At mild temperature abuse (10 degrees C), toxin was detected by day 14, also independent of packaging atmosphere. At refrigeration temperature (4 degrees C), toxin was detected by day 14 in product packaged under 100% N2 and by day 28 in product packaged under 30% CO2:70% N2. Reduced storage temperature significantly delayed toxin production and extended the period of sensory acceptability of cooked turkey, but even strict refrigeration did not prevent growth and toxigenesis by nonproteolytic C. botulinum. At all three storage temperatures, toxin detection preceded or coincided with development of sensory characteristics of spoilage, demonstrating the potential for consumption of toxic product when spoilage-signaling sensory cues are absent.     

Carbon Dioxide Evolution and Moisture Evaporation During Roasting of Coffee Beans

ABSTRACT: Evolution of carbon dioxide and water vapor during roasting of coffee was followed in an isothermal high-temperature short-time and a low-temperature long-time roasting process. In addition, CO₂ release during storage of roasted beans was followed. CO₂ and water vapor concentration were assayed in the exhaust air by nondispersive infrared gas analysis. Although CO₂ evolution rates differed in the 2 processes, the final total amount of CO₂ released after 63 d of storage remained equal. CO₂ evolution and differentiation between evaporation of initial water and chemically formed water showed that chemical reactions leading to relevant amounts of CO₂ and water start at ap -proximately 180°C. A mass balance established on the present measurements was able to account fairly well for the gravimetrically measured roast loss.     

Evaluation and assessment of lumbar load during total shifts for occupational manual materials handling jobs within the Dortmund Lumbar Load Study – DOLLY

The load on the lumbar spine during occupational manual materials handling was previously investigated with respect to short activity sections or to specified load-handling types such as lifting or carrying. Within the so-called Dortmund Lumbar Load Study, analysis of the occupationally induced load on the lumbar spine during total working shifts in the field of surface construction, drop forge, industrial meat processing, and refuse collection was performed on the shop-floor. The body postures adopted, the action forces applied at the hands, and the resultant lumbar load for all load-handling tasks were analysed for 2 shifts in each field on the basis of video evaluations. Via a newly developed detailed classification procedure, the spatial position of the body segments as well as amplitude and direction of the action forces were described in a detailed manner. Consecutive biomechanical model calculations lead, for total shifts, to time courses of various measures for the load on the lumbar spine, such as flexion or torsional moments of force as well as compression and shear forces at the lumbosacral disc. In relation to recommended limits for the maximal disc compression provided in the literature, lumbar load is exceeded in numerous situations during a shift, in particular, with regard to persons of higher age. In a “dose model” applied in this study, the cumulative effect of single-task exposures was considered by superproportional weighting of the compressive force with respect to the corresponding duration of a working task.

Relevance to industry

A comprehensive evaluation of lumbar load for complete shifts is presented considering the real shop-floor conditions. Analyses for dustbin removal, surface construction and industrial meat processing have discovered numerous exceedings of lumbar-load limits. Such tasks should not be performed by older persons from the preventive point of view.     

Wege zur Absicherung eines Intranets

Zusammenfassung Ziel dieses Artikels ist es, einen überblick über technische Sicherheitsl?sungen für Intranets zu geben. Ein Modell, das die Anforderungen an ein Sicherheitssystem in Form von abstrakten Diensten abbildet, dient dazu, verschiedene L?sungsalternativen zur Realisierung der unterschiedlichen Dienste auf Basis offener Standards oder generalisierter Produktklassen beurteilen zu k?nnen. Darauf aufbauend wird eine beispielhafte Referenzarchitektur für Unternehmen auf Basis einer X.509 Public-Key-Infrastruktur entworfen.      

Direct Isomerization of Linoleic Acid to Conjugated Linoleic Acids (CLA) using Gold Catalysts

Supported gold catalysts, e.g., Au on Al₂O₃, Fe₂O₃, CeO₂, MnO₂, TiO₂, ZrO₂, activated carbon, titanium silicalite TS‐1, were prepared and used for the isomerization of linoleic acid (cis‐9,cis‐12‐octadecadienoic acid) to conjugated linoleic acids (CLA) in the presence of hydrogen at 165 °C in a batch reactor. The best results were obtained using a catalyst with 2 wt % Au on TS‐1, which exhibits a high selectivity (78 %) towards CLA. The two biologically active target CLA isomers, i.e., cis‐9,trans‐11‐CLA and trans‐10,cis‐12‐CLA, were the main products. During the isomerization of linoleic acid to CLA, consecutive reactions also took place. These were the hydrogenation of linoleic acid and CLA to monounsaturated octadecenoic acids and the further hydrogenation of monounsaturated acids to stearic acid. Thus, gold catalysts are capable of isomerizing linoleic acid to CLA and hydrogenating their double bonds to an extent that depends on the Au catalyst used.     

Lateralized processing of speech prosodies in the temporal cortex: a 3-T functional magnetic resonance imaging study

Prosodic modulation of speech provides information about emotional states of speakers (affective prosodies) or serves as syntactic markers to change linguistic aspects of speech (linguistic prosodies). Previous electrophysiological investigations and studies on patients with right or left hemisphere damage showed nonuniform results with respect to lateralization of prosodic processing. In this study 20 healthy right-handed volunteers were investigated with functional magnetic resonance imaging of the acoustically responsive areas on the supratemporal plane while detecting phonemes as control targets or prosodies in strings of nonsense syllables and adjectives, the latter randomly intonated in a declarative, interrogative, commanding, happy, or sad fashion. In control task A the phoneme /a/ was detected in the syllables. In control task B the phoneme /a/ was detected in the adjectives, and in the experimental task C the sad intonations (affective) and in the experimental task D the interrogative intonations (linguistic) had to be detected in the same material. In task A intensity-weighted volumes of activated voxels were not different in the two hemispheres (laterality index 0). In task B with an irrelevant phoneme detection with respect to prosodic material, the population split into two subgroups with similar right or left hemispheric lateralization of activity leading to an absolute laterality index of 26.8 across all subjects. During detection of affective prosodies (task C), lateralization was maintained yet the absolute laterality index reduced to 14.5, while there was no lateralization during detection of linguistic prosodies. The sum of activations in the two hemispheres was the same across all tasks and subgroups, which suggests that the lateralizations occurring with presentation and detection of prosodic material depend on a redistribution of activity between hemispheres.     

Rankings und „Wo studieren die Besten?“

Ein Ranking beruht meist auf einer Abbildung einer endlichen Menge von (hochkomplexen) Strukturen auf die reelle Achse.     

ANALYSIS OF HOP EXTRACTS BY SIMPLIFIED COUNTER-CURRENT DISTRIBUTION METHODS

Simplified counter-current distribution procedures are described which allow correct analysis of the α acids and iso α acids content of hop extracts.     

Impact of Annual Average Daily Traffic on Highway Runoff Pollutant Concentrations

The objective of this study was to evaluate correlations between annual average daily traffic (AADT) and storm water runoff pollutant concentrations generated from California Department of Transportation (Caltrans) highway sites. Analyses of data collected from the Caltrans four-year (1997–2001) highway runoff characterization program revealed that, in general, pollutant concentrations from urban highways were higher than those found from nonurban highways. For a limited number of pollutants, however, the concentrations from nonurban highways were found to be higher than the concentrations from urban highways. No direct linear correlation was found between highway runoff pollutant event mean concentrations and AADT. However, through multiple regression analyses, it was shown that AADT has an influence on most highway runoff constituent concentrations, in conjunction with factors associated with watershed characteristics and pollutant build-up and wash off. The other noticeable factors shown to influence the accumulation of pollutants on highways were antecedent dry period, drainage area, maximum rain intensity, and land use.