Generalizing from single target search to multiple target search

This study was conducted to generalize from models of human performance in single target search to multiple target search. The specific issue was exhaustive search for the complete set of targets on a search field, extending the work of Chan and Chan from a two-target exhaustive search. Mathematical modelling was based on earlier derivations of search for the first of a set of targets. Model and simulation results showed three quite different relationships between mean search time and the number of targets remaining in a search field. The human search strategy for multiple targets was close to the systematic strategy for the first few targets and was close to the random strategy for the last few targets. Plotting mean search time for multiple targets against the number of targets remaining would be a useful and unique way to show human use of systematic search strategy.     

ISSN exercise & sport nutrition review: research & recommendations

Sports nutrition is a constantly evolving field with hundreds of research papers published annually. For this reason, keeping up to date with the literature is often difficult. This paper is a five year update of the sports nutrition review article published as the lead paper to launch the JISSN in 2004 and presents a well-referenced overview of the current state of the science related to how to optimize training and athletic performance through nutrition. More specifically, this paper provides an overview of: 1.) The definitional category of ergogenic aids and dietary supplements; 2.) How dietary supplements are legally regulated; 3.) How to evaluate the scientific merit of nutritional supplements; 4.) General nutritional strategies to optimize performance and enhance recovery; and, 5.) An overview of our current understanding of the ergogenic value of nutrition and dietary supplementation in regards to weight gain, weight loss, and performance enhancement. Our hope is that ISSN members and individuals interested in sports nutrition find this review useful in their daily practice and consultation with their clients.     

Assessing the Floristic Diversity of Hedge Networks: A landscape perspective

The type of land cover adjacent to hedges has been shown to influence the species composition of the ground flora. An assessment of the contribution of hedge networks to the biodiversity of landscapes therefore requires information on the relative lengths of hedge associated with different land covers. In the River Tyne catchment, Northern England, the association between hedges and land cover types differed between four landscapes. Overall, the greatest length of hedge was adjacent to arable crops, improved grasslands and roads. Hedges next to improved grassland and roads were the most species-rich, while those adjacent to arable crops were the least species-rich. Species which were common adjacent to arable crops were also common adjacent to other land covers. Hedges adjacent to improved grassland, roads and semi-natural broadleaved woodland supported species which were not common elsewhere in the hedge network. It was also found that hedges with different land cover on each side supported different floras on each side. When planning landscape-scale surveys of hedge biodiversity, it is important to survey both sides of hedges, and sampling should be stratified to include hedges adjacent to the different types of land cover present.     

Electrokinetic geosynthetics in hydraulic applications

In use most geosynthetics play a passive role. New applications for geosynthetics have been identified if they can provide an active role, initiating biological, chemical or physical change to the matrix in which it is installed as well as providing the established functions. This can be achieved by combining the electrokinetic phenomena of electro-osmosis, electrophoresis and associated electrokinetic functions such as electrolysis with the traditional functions of geosynthetics of drainage, filtration, containment and reinforcement to form electokinetic geosynthetics (EKG). Electrokinetic geosynthetics can be made singly or from combinations of woven, non-woven, needle punched knitted, extruded or laminated materials and can be formed in any 2D or 3D shape.The majority of the uses of EKG are in hydraulic applications or applications with a significant hydraulic component. These can be grouped in separate engineering categories such as civil, mining, and water engineering. The concept of electrokinetic geosynthetics is described and details of applications and case studies are provided in the paper.     

Biomechanical properties of single chondrocytes and chondrons determined by micromanipulation and finite-element modelling

A chondrocyte and its surrounding pericellular matrix (PCM) are defined as a chondron. Single chondrocytes and chondrons isolated from bovine articular cartilage were compressed by micromanipulation between two parallel surfaces in order to investigate their biomechanical properties and to discover the mechanical significance of the PCM. The force imposed on the cells was measured directly during compression to various deformations and then holding. When the nominal strain at the end of compression was 50 per cent, force relaxation showed that the cells were viscoelastic, but this viscoelasticity was generally insignificant when the nominal strain was 30 per cent or lower. The viscoelastic behaviour might be due to the mechanical response of the cell cytoskeleton and/or nucleus at higher deformations. A finite-element analysis was applied to simulate the experimental force-displacement/time data and to obtain mechanical property parameters of the chondrocytes and chondrons. Because of the large strains in the cells, a nonlinear elastic model was used for simulations of compression to 30 per cent nominal strain and a nonlinear viscoelastic model for 50 per cent. The elastic model yielded a Young''s modulus of 14 ± 1 kPa (mean ± s.e.) for chondrocytes and 19 ± 2 kPa for chondrons, respectively. The viscoelastic model generated an instantaneous elastic modulus of 21 ± 3 and 27 ± 4 kPa, a long-term modulus of 9.3 ± 0.8 and 12 ± 1 kPa and an apparent viscosity of 2.8 ± 0.5 and 3.4 ± 0.6 kPa s for chondrocytes and chondrons, respectively. It was concluded that chondrons were generally stiffer and showed less viscoelastic behaviour than chondrocytes, and that the PCM significantly influenced the mechanical properties of the cells.     

Levels of polybrominated diphenyl ether (PBDE) flame retardants in animals representing different trophic levels of the North Sea food Web

The levels of individual PBDE congeners were investigated in the invertebrate species whelk (Buccinum undatum), seastar (Asterias rubens), and hermit crab (Pagurus bernhardus), the gadoid fish species whiting (Merlangius merlangus) and cod (Gadus morhua), and the marine mammal species harbor seal (Phoca vitulina) and harbor porpoise (Phocoena phocoena). These species are all important representatives of different trophic levels of the North Sea food web. All six major PBDE congeners detected (BDEs 28, 47, 99, 100, 153, and 154) are most prevalent in the commercial Penta-BDE formulation. There is no evidence for the occurrence of the Octa-BDE formulation in the North Sea food web, since its dominant congener, BDE183, was never detected. BDE209, the main congener (> 97%) in the Deca-BDE formulation, was detected only in a minority of the samples and always in concentrations around the limit of detection. Since BDE209 is often the major BDE congener in sediments from the area, the main reason for its low concentrations in biota from the North Sea seems to be a relatively low bioaccumulation potential. This can either be due to a low uptake rate of the very large molecule or a relatively rapid excretion after biotransformation. Since all invertebrates investigated are sentinel species, they are highly representative for the area of capture. The highest lipid-normalized concentrations of PBDEs in the invertebrates occurred near the mouth of the river Tees at the East coast of the UK. The geographical distribution of the PBDEs can be explained by the residual currents in the area. The direction of these currents differs between the summer and the winter season as a result of the presence or absence of vertical summer stratification of the deeper waters north of the Dogger Bank. Summer stratification results in the development of a density-driven bottom water current formed after the onset of vertical stratification of the water column in May leaving the UK coast near Flamborough Head toward the Dogger Bank. In winter, the residual currents run in a more southerly direction and follow the UK coastline. The distribution pattern of the PCBs and p,p'-DDE in the invertebrates was entirely different from that of the PBDEs, which could be expected, since the use of these organochlorines in western Europe peaked in the 1960s and 1970s but has been forbidden more than two decades ago, whereas the production and use of the penta-BDE formulation is of a more recent origin. The higher trophic levels of the North Sea food web were represented by the predatory gadoid fish species whiting and cod and the marine mammal species harbor seal and harbor porpoise. The lipid-normalized levels of the six major PBDE congeners in fish were similar to the levels in the invertebrates, but a biomagnification step in concentrations of generally more than an order of magnitude occurred from gadoid fish to marine mammals. Based on the limited number of samples, no differences could be observed between harbor seal and harbor porpoise. In summary, the results in three species of sentinel invertebrates from a network of stations covering a major part of the North Sea basin showed that the estuary of the river Tees at the UK East coast is a major source for tri- to hexa-PBDEs. Throughout the food-chain, the most marked increase in (lipid-normalized) levels of all six PBDE congeners occurred from predatory (gadoid) fish to marine mammals, agreeing with the transition from gill-breathing to lung-breathing animals. This has serious consequences for the route of elimination of POPs, since their elimination from the blood into the ambient seawater via the gill-membrane is no longer possible.     

Three-dimensional phase imaging with the intensity transport equation

Phase can be retrieved from intensity measurements with the intensity transport equation. Three-dimensional image formation of weak phase objects based on this method is investigated. It is shown that, although the refractive index of a thin object can be measured, the three-dimensional variation of refractive index of an arbitrary object cannot, in general, be reconstructed, as spatial frequencies with a zero-axial component are not detected. However, this may not be a problem if regions with known refractive index are present in the sample.     

Quantitative analysis of mitoxantrone by surface-enhanced resonance Raman scattering

Mitoxantrone is an anticancer agent for which it is important to know the concentration in blood during therapy. Current methods of analysis are cumbersome, requiring a pretreatment stage. A method based on surface-enhanced resonance Raman scattering (SERRS) has been developed using a flow cell and silver colloid as the SERRS substrate. It is simple, sensitive, fast, and reliable. Both blood plasma and serum can be analyzed directly, but fresh serum is preferred here due to reduced fluorescence in the clinical samples available. Fluorescence is reduced further by the dilution of the serum in the flow cell and by quenching by the silver of surface-adsorbed material. The effectiveness of the latter process is dependent on the contact time between the serum and the silver. The linear range encompasses the range of concentrations detected previously in patient samples using HPLC methods. In a comparative study of a series of samples taken from a patient at different times, there is good agreement between the results obtained by HPLC and SERRS with no significant difference between them at the 95% limit. The limit of detection in serum using the final optimized procedure for SERRS was 4.0 x 10(-11) M (0.02 ng/mL) mitoxantrone. The ease with which the SERRS analysis can be carried out makes it the preferred choice of technique for mitoxantrone analysis.     

Physical modelling of solute transport in porous media: evaluation of an imaging technique using UV excited fluorescent dye

The development and evaluation of a 2-dimensional physical model, which is designed to assist in the characterisation of complex solute transport problems in porous media, is described. The laboratory model is a transparent 2-dimensional porous media of nominal thickness and uses a non-invasive imaging technique in conjunction with a fluorescent dye tracer (sodium fluorescein) to monitor solute movements. Under ultraviolet (UV) illumination the dye emits visible light which is imaged by a CCD (Charge Coupled Device) camera. The image is processed to estimate the 2-dimensional distribution of tracer concentrations. The system can successfully model a simple contaminant plume within a homogenous porous matrix constructed from glass beads (60-100 microm). Experimental results show that transverse dispersion coefficient was 3.9 x 10(-10) m2/s when sodium fluorescein transported in porous matrix with a walter velocity of 5.71 x 10(-6) m/s. The low transverse dispersion coefficient suggests that the molecular diffusion of solute cannot be neglected under low velocity of the water. The advantages of using UV rather than an ordinary light system are a reduction in noise and experimental errors. Errors due to light dispersion within the model are shown to be negligible for the current model. Since contaminant with aromatic rings are usually fluorescent and biological samples can be labelled by fluorescent dye, this imaging technique using UV excited fluorescent dye will be used to investigate biodegradation process in porous media.