Macrophage migration inhibitory factor is a neuroendocrine mediator of endotoxaemia

The cytokine macrophage migration inhibitory factor (MIF) is a major protein constituent of the anterior pituitary gland released into the bloodstream during endotoxaemia. For many years, MIF had been thought to be a T cell product associated with delayed-type hypersensitivity reactions. The identification of MIF as a pituitary 'stress' hormone provides an important link in the regulation of systemic inflammatory responses by the central nervous system.     

Sequence-specific methylation of the mouse H19 gene in embryonic cells deficient in the Dnmt-1 gene

Currently, the pig species is regarded as the most likely organ donor for human xenotransplantation in the future. However, it cannot be granted that the pig will be the optimal species of choice. We have studied human anti-sheep antibodies in comparison with anti-pig antibodies. The anti-sheep lymphocytotoxic and hemagglutination titers were in the range 8 to 128 and 2 to 32, respectively, in single individuals, which were considerably lower than the anti-pig titers of these individuals. Perfusion of sheep kidneys with human blood reduced the anti-sheep xenoantibody titers to zero as measured by lymphocytotoxic, hemagglutination, and sheep aortic endothelial cell antibody binding assays. The perfused kidneys showed generalised depositions of human IgM and C3c in the vascular tree and focal depositions of C1q and fibrin. Obliteration of capillaries by human platelets and polymorphonuclear cells were observed. Total neutral glycolipid fractions were isolated from sheep intestinal, pancreatic, and kidney tissues. By using a chromatogram binding assay, a monoclonal anti-Forssman antibody identified a single compound with five sugar residues in all organs. Several glycolipid bands were stained in all organs by the Gal(alpha)1-specific lectin I-B4 from Griffonia (Bandeiraea) Simplicifolia. A human AB serum pool showed staining by both IgG and IgM antibodies of the Forssman and Gal(alpha)1-terminating components as well as some other, not structurally identified, components. The Forssman and Gal(alpha)1-reactivity in human sera could be eliminated by immunoadsorption using Forssman and Gal(alpha)1-3Gal-immunoadsorbent columns, respectively. Immunostaining of sheep kidney tissue sections showed the presence of Gal(alpha)1-terminating epitopes by immunoperoxidase and immunogold silver staining techniques. Proximal convoluted tubules showed a strong staining, while thin loops of Henle, collecting ducts, urothelium, and vessels showed a weaker staining. Distal convoluted tubules and thick loops of Henle were completely negative. In summary, human serum contains anti-sheep xenoantibodies reacting mainly with the Forssman and Gal(alpha)1-determinants in sheep tissues and the anti-sheep antibody titers are lower than the corresponding anti-pig titers.     

Efficient immune responses in mice lacking N-region diversity

Mice with a null mutation in the terminal deoxynucleotidyl transferase (TdT) gene harbor immunoglobulin and T cell receptor repertoires essentially devoid of N-region diversity. Consequently, the CDR3 loops important for antigen recognition are shorter and considerably less diverse than those of wild-type controls. We find surprisingly normal immune responses in TdT0 mice, as regards both efficiency and specificity. This provokes a reconsideration of the assumption that N-region diversity is required for an effective T and B cell repertoire.     

A new technology for preparation of saturating powder mixtures for diffusion galvanizing

The powder mixtures used for diffusion galvanizing (Zn + inert filler + activator) have the following deficiencies: susceptibility to sintering and fusing under elevated CTT temperatures, complexity of correcting the set of components in repeated use of the mixtures and the related instability of the results obtained, complexity of regeneration of worked-out mixtures, elevated ecological hazard of their use, etc. This explains the need for the present work, which shows that most of the mentioned deficiencies can be eliminated by an appropriate choice of the mixture components and (or) preliminary treatment of them.     

Modulation of drug-induced sensitization processes by endogenous opioid systems

Behavioural sensitization involves progressive increases in behavioural responses to repeated intermittent administration of drugs of abuse. Behavioural sensitization is observed to the locomotor stimulant, rewarding and discriminative effects of a drug. These are effects which seem to be essential in the initiation, expression and maintenance of a drug-seeking behaviour. Therefore the phenomenon of behavioural sensitization may have important implications for the understanding of addictive processes. Findings given in this review demonstrate the involvement of endogenous opioid systems in the initiation of sensitized responses on the neurochemical level, i.e., within the mesolimbic dopaminergic system, as well as on the behavioural level. Specifically, it is shown that behavioural sensitization to morphine and cocaine is modulated by endogenous kappa-opioid systems.     

Making broadband-ISDN successful

It is argued that the technology needed to support the emerging broadband integrated services digital network technology (B-ISDN) is on the horizon, but the deployment of this technology in the telecommunication plant will not be sufficient to make B-ISDN a commercial success. Two corequisites for ensuring the long-term success of the emerging B-ISDN include stimulating the demand for bandwidth by inventing and promoting new telecommunication applications, and enabling the interconnection or convergence of the emerging high-speed network and the existing cable television infrastructure. The author believes that this is necessary because no alternative seems to offer a workable base for financing the transition to B-ISDN and for reaching the needed economy of scale, and because the intrinsic value of the public network grows combinatorially with the number of subscribers     

Block access estimation for clustered data using a finite LRUbuffer

Data access cost evaluation is fundamental in the design and management of database systems. When some data items have duplicates, a clustering effect that can heavily influence access costs is observed. The availability of a finite amount of buffer memory in real systems has an even more dramatic impact. A comprehensive cost model for clustered data retrieval by an index using a finite buffer is presented. The approach combines and extends previous models based either on finite buffer or on uniform data clustering assumptions. The computational costs of the formulas proposed are independent of the data size or of the query cardinality and need only a single statistics per search key, the clustering factor, to be maintained by the system. The predictive power and the accuracy of the model are shown in comparison with actual costs resulting from simulations