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101.
A. Tremel N. Tirtaatmadja G.W. Stevens A.J. O’Connor 《Chemical engineering science》2009,64(2):247-43
The interaction of living cells with surfaces is important in applications of biomaterials, such as tissue engineering. Characterising and modelling the attachment, migration and proliferation of cells on materials used for tissue engineering provides valuable insight into their potential applications as well as a means of objective comparison. In this study, proliferation and migration of NIH-3T3 fibroblast cells on tissue culture plastic in vitro were quantified. The development of randomly scattered individual cells into confluent cell monolayers proceeded with cell density following a logistic growth pattern. Travelling cell wavefronts produced in a wound healing assay were modelled with a modified Fisher equation incorporating both diffusion and logistic growth. The diffusivity and growth rates thus determined could be used for comparison with cell behaviour on other surfaces or under different conditions. Cell tracking showed that the average effective velocity of cells varied inversely with cell density, supporting contact inhibition of cell movement. 相似文献
102.
The use of High Pressure Grinding Rollers (HPGR) has been widely reported to have major benefits in the treatment of minerals such as iron ore and diamonds. To date there have been few investigations into its use in the treatment of ores containing Platinum-Group Minerals (PGMs). HPGRs are known to reduce energy consumption and wear costs and improve the throughput in the circuit. In the present investigation the effect of the comparative use of HPGR and conventional crushing in combination with either dry or wet rod milling on the flotation of PGMs was studied using batch flotation. Previous studies of a base metal sulphide had shown that either HPGR or conventional crushing followed by dry milling produced the highest grades and recoveries (Palm et al., 2010). However in the present study it was observed that a similar treatment of Platinum-Group Minerals produced the poorest results and the highest grades and recoveries were obtained for the case of conventional crushing in combination with wet milling. The HPGR showed no advantages in terms of flotation performance and dry milling produced particularly poor flotation results.The results were investigated further using various surface characterization techniques in order to determine the reason for the decrease in grades and recoveries of platinum when using dry milling and HPGR as opposed to the case for base metal sulphides. The feed and product samples were analysed using ToF-SIMS, XPS and MLA. The paper will propose reasons to explain the different flotation behaviour of the two ore types following the various comminution processes focusing on the surface characteristics of the ores, the particle size distribution and the pulp chemistry. 相似文献
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Hepatitis C, which is caused by the hepatitis C virus (HCV), is a major public health problem in the United States. HCV is most efficiently transmitted through large or repeated percutaneous exposures to blood. Most patients with acute HCV infection develop persistent infection, and 70 percent of patients develop chronic hepatitis. HCV-associated chronic liver disease results in 8,000 to 10,000 deaths per year, and the annual costs of acute and chronic hepatitis C exceed $600 million. An estimated 3.9 million Americans are currently infected with HCV, but most of these persons are asymptomatic and do not know they are infected. To identify them, primary health care professionals should obtain a history of high-risk practices associated with the transmission of HCV and other bloodborne pathogens from all patients. Routine testing is currently recommended only in patients who are most likely to be infected with HCV. 相似文献
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Tristan I. Croll Kilian Mueller Andrea J. O’Connor Justin J. Cooper-White 《Chemical engineering science》2005,60(17):4924-4934
Soft tissue engineering presents significant challenges compared to other tissue engineering disciplines such as bone, cartilage or skin engineering. The very high cell density in most soft tissues, often combined with large implant dimensions, means that the supply of oxygen is a critical factor in the success or failure of a soft tissue scaffold. A model is presented for oxygen diffusion in a 15-60 mm diameter dome-shaped scaffold fed by a blood vessel loop at its base. This model incorporates simple models for vascular growth, cell migration and the effect of cell density on the effective oxygen diffusivity. The model shows that the dynamic, homogeneous cell seeding method often employed in small-scale applications is not applicable in the case of larger scale scaffolds such as these. Instead, we propose the implantation of a small biopsy of tissue close to a blood supply within the scaffold as a technique more likely to be successful. 相似文献
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