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Manuela Costanzo Elisabetta Esposito Maddalena Sguizzato Maria Assunta Lacavalla Markus Drechsler Giuseppe Valacchi Carlo Zancanaro Manuela Malatesta 《International journal of molecular sciences》2021,22(10)
In this pilot study, ethosomes and transethosomes were investigated as potential delivery systems for cholecalciferol (vitamin D3), whose deficiency has been correlated to many disorders such as dermatological diseases, systemic infections, cancer and sarcopenia. A formulative study on the influence of pharmaceutically acceptable ionic and non-ionic surfactants allowed the preparation of different transethosomes. In vitro cytotoxicity was evaluated in different cell types representative of epithelial, connective and muscle tissue. Then, the selected nanocarriers were further investigated at light and transmission electron microscopy to evaluate their uptake and intracellular fate. Both ethosomes and transethosomes proven to have physicochemical properties optimal for transdermal penetration and efficient vitamin D3 loading; moreover, nanocarriers were easily internalized by all cell types, although they followed distinct intracellular fates: ethosomes persisted for long times inside the cytoplasm, without inducing subcellular alteration, while transethosomes underwent rapid degradation giving rise to an intracellular accumulation of lipids. These basic results provide a solid scientific background to in vivo investigations aimed at exploring the efficacy of vitamin D3 transdermal administration in different experimental and pathological conditions. 相似文献
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Costanzo GA Siccardi M Barychev V De Marchi A 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2000,47(1):249-255
The high C-field Cs beam frequency standard is presently a working machine that is undergoing first evaluations. The projected 10(-14 ) accuracy goal is as yet unattained, mainly because of inadequate C-field uniformity and stability. An analysis of the projected possible C-field improvements and the consequent uncertainty is here reported. 相似文献
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P Vittorioso R Cowling JD Faure M Caboche C Bellini 《Canadian Metallurgical Quarterly》1998,18(5):3034-3043
The pasticcino (pas) mutants of Arabidopsis thaliana are a new class of plant developmental mutants; members of this class show ectopic cell proliferation in cotyledons, extra layers of cells in the hypocotyl, and an abnormal apical meristem. This phenotype is correlated with both cell division and cell elongation defects. There are three complementation groups of pas mutants (pas1, pas2, and pas3, with, respectively 2, 1, and 4 alleles). Here we describe in more detail the pas1-1 allele, which was obtained by insertional mutagenesis. The PAS1 gene has been cloned and characterized; it encodes an immunophilin-like protein similar to the p59 FK506-binding protein (FKBP52). PAS1 is characterized by an FKBP-like domain and three tetratricopeptide repeat units. Although the presence of immunophilins in plants has already been demonstrated, the pas1-1 mutant represents the first inactivation of an FKBP-like gene in plants. PAS1 expression is altered in pas1 mutants and in the pas2 and pas3 mutants. The expression of the PAS1 gene is increased in the presence of cytokinins, a class of phytohormones originally discovered because of their ability to stimulate cell division. These results are of particular relevance as they show for the first time that an FKBP-like protein plays an important role in the control of plant development. 相似文献
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M Seo S Akaba T Oritani M Delarue C Bellini M Caboche T Koshiba 《Canadian Metallurgical Quarterly》1998,116(2):687-693
Aldehyde oxidase (AO; EC 1.2.3.1) activity was measured in seedlings of wild type or an auxin-overproducing mutant, superroot1 (sur1), of Arabidopsis thaliana. Activity staining for AO after native polyacrylamide gel electrophoresis separation of seedling extracts revealed that there were three major bands with AO activity (AO1-3) in wild-type and mutant seedlings. One of them (AO1) had a higher substrate preference for indole-3-aldehyde. This AO activity was significantly higher in sur1 mutant seedlings than in the wild type. The difference in activity was most apparent 7 d after germination, the same time required for the appearance of the remarkable sur1 phenotype, which includes epinastic cotyledons, elongated hypocotyls, and enhanced root development. Higher activity was observed in the root and hypocotyl region of the mutant seedlings. We also assayed the indole-3-acetaldehyde oxidase activity in extracts by high-performance liquid chromatography detection of indole-3-acetic acid (IAA). The activity was about 5 times higher in the extract of the sur1 seedlings, indicating that AO1 also has a substrate preference for abscisic aldehyde. Treatment of the wild-type seedlings with picloram or IAA caused no significant increase in AO1 activity. This result suggested that the higher activity of AO1 in sur1 mutant seedlings was not induced by IAA accumulation and, thus, strongly supports the possible role of AO1 in IAA biosynthesis in Arabidopsis seedlings. 相似文献
17.
An integrated probe performing phaseless near-field measurements on a polar surface is presented. The unknown near-field phase is computed by an interferometric technique used in conjunction with the minimisation of a suitable functional. A microstrip patch antenna for synthetic aperture radar (SAR) applications is considered to validate the method 相似文献
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Several techniques were presented in the literature in order to perform accurate tracking of frequencies for different purposes. Most of them are modified versions of the traditional discrete Fourier transform (DFT) or other spectrum-estimation techniques. This paper presents a procedure based on the statistical analysis of the current signal in the time domain, referred to as maximum covariance method for frequency tracking (MCMFT), which allows to obtain high-frequency resolution independent of the sampling frequency and of the time acquisition period. Owing to the proposed procedure, the spectrum lines related to supply frequency or to rotor-slotting frequency for an induction machine can be detected with extreme accuracy within a wide range of sampled data conditions. Then, an accurate slip computation, or speed estimation, for sensorless control or distribution network diagnosis can be performed. Comparisons between previously existing methods and the proposed one are reported, in order to critically analyze its performances. Two different-sized induction machines were used for the experiments. 相似文献
20.
With the spread of computer technology into the artistic fields, new application scenarios for computer-based applications of symbolic music representation (SMR) have been identified. The integration of SMR in a versatile multimedia framework such as MPEG will enable the development of a huge number of new applications in the entertainment, education, and information delivery domains. 相似文献