全文获取类型
收费全文 | 3266篇 |
免费 | 229篇 |
国内免费 | 3篇 |
专业分类
电工技术 | 16篇 |
综合类 | 2篇 |
化学工业 | 1216篇 |
金属工艺 | 29篇 |
机械仪表 | 83篇 |
建筑科学 | 107篇 |
矿业工程 | 1篇 |
能源动力 | 80篇 |
轻工业 | 995篇 |
水利工程 | 16篇 |
石油天然气 | 15篇 |
无线电 | 99篇 |
一般工业技术 | 398篇 |
冶金工业 | 89篇 |
原子能技术 | 5篇 |
自动化技术 | 347篇 |
出版年
2024年 | 10篇 |
2023年 | 49篇 |
2022年 | 234篇 |
2021年 | 259篇 |
2020年 | 123篇 |
2019年 | 121篇 |
2018年 | 135篇 |
2017年 | 149篇 |
2016年 | 134篇 |
2015年 | 108篇 |
2014年 | 181篇 |
2013年 | 266篇 |
2012年 | 252篇 |
2011年 | 246篇 |
2010年 | 154篇 |
2009年 | 170篇 |
2008年 | 162篇 |
2007年 | 160篇 |
2006年 | 96篇 |
2005年 | 92篇 |
2004年 | 69篇 |
2003年 | 58篇 |
2002年 | 45篇 |
2001年 | 28篇 |
2000年 | 28篇 |
1999年 | 20篇 |
1998年 | 27篇 |
1997年 | 28篇 |
1996年 | 23篇 |
1995年 | 13篇 |
1994年 | 6篇 |
1993年 | 7篇 |
1992年 | 4篇 |
1991年 | 8篇 |
1990年 | 1篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1987年 | 4篇 |
1985年 | 3篇 |
1984年 | 5篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1976年 | 2篇 |
1975年 | 1篇 |
1972年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有3498条查询结果,搜索用时 15 毫秒
61.
Stefania Mamberti Maruthi K. Pabba Alexander Rapp M. Cristina Cardoso Michael Scholz 《International journal of molecular sciences》2022,23(7)
CTCF is a nuclear protein initially discovered for its role in enhancer-promoter insulation. It has been shown to play a role in genome architecture and in fact, its DNA binding sites are enriched at the borders of chromatin domains. Recently, we showed that depletion of CTCF impairs the DNA damage response to ionizing radiation. To investigate the relationship between chromatin domains and DNA damage repair, we present here clonogenic survival assays in different cell lines upon CTCF knockdown and ionizing irradiation. The application of a wide range of ionizing irradiation doses (0–10 Gy) allowed us to investigate the survival response through a biophysical model that accounts for the double-strand breaks’ probability distribution onto chromatin domains. We demonstrate that the radiosensitivity of different cell lines is increased upon lowering the amount of the architectural protein. Our model shows that the deficiency in the DNA repair ability is related to the changes in the size of chromatin domains that occur when different amounts of CTCF are present in the nucleus. 相似文献
62.
Maria Cristina Samson Mariolina Gullì Nelson Marmiroli 《Journal of the science of food and agriculture》2010,90(9):1437-1444
BACKGROUND: Methodologies that enable the detection of genetically modified organisms (GMOs) (authorized and non‐authorized) in food and feed strongly influence the potential for adequate updating and implementation of legislation together with labeling requirements. Quantitative polymerase chain reaction (qPCR) systems were designed to boost the sensitivity and specificity on the identification of GMOs in highly degraded DNA samples; however, such testing will become economically difficult to cope with due to increasing numbers of approved genetically modified (GM) lines. Multiplexing approaches are therefore in development to provide cost‐efficient solution. RESULTS: Construct‐specific primers and probe were developed for quantitative analysis of Roundup Ready ® soybean (RRS) event glyphosate‐tolerant soybean (GTS) 40‐3‐2. The lectin gene (Le1) was used as a reference gene, and its specificity was verified. RRS‐ and Le1‐specific quantitative real‐time PCR (qRTPCR) were optimized in a duplex platform that has been validated with respect to limit of detection (LOD) and limit of quantification (LOQ), as well as accuracy. The analysis of model processed food samples showed that the degradation of DNA has no adverse or little effects on the performance of quantification assay. CONCLUSION: In this study, a duplex qRTPCR using TaqMan minor groove binder‐non‐fluorescent quencher (MGB‐NFQ) chemistry was developed for specific detection and quantification of RRS event GTS 40‐3‐2 that can be used for practical monitoring in processed food products. Copyright © 2010 Society of Chemical Industry 相似文献
63.
Donoso-Bravo A Mailier J Martin C Rodríguez J Aceves-Lara CA Vande Wouwer A 《Water research》2011,45(17):5347-5364
Anaerobic digestion enables waste (water) treatment and energy production in the form of biogas. The successful implementation of this process has lead to an increasing interest worldwide. However, anaerobic digestion is a complex biological process, where hundreds of microbial populations are involved, and whose start-up and operation are delicate issues. In order to better understand the process dynamics and to optimize the operating conditions, the availability of dynamic models is of paramount importance. Such models have to be inferred from prior knowledge and experimental data collected from real plants. Modeling and parameter identification are vast subjects, offering a realm of approaches and methods, which can be difficult to fully understand by scientists and engineers dedicated to the plant operation and improvements. This review article discusses existing modeling frameworks and methodologies for parameter estimation and model validation in the field of anaerobic digestion processes. The point of view is pragmatic, intentionally focusing on simple but efficient methods. 相似文献
64.
Deiweson Souza-Monteiro Mrcia Cristina dos Santos Guerra Leonardo Oliveira Bittencourt Walessa Alana Bragana Arago Aline Dionizio Felipe Martins Silveira Marília Afonso Rebelo Buzalaf Manoela Domingues Martins Maria Elena Crespo-Lopez Rafael Rodrigues Lima 《International journal of molecular sciences》2022,23(4)
Aluminum (Al) is one of the most abundant elements on Earth, and its high extraction rate and industrial use make human exposure very common. As Al may be a human toxicant, it is important to investigate the effects of Al exposure, mainly at low doses and for prolonged periods, by simulating human exposure. This work aimed to study the effects of low-dose exposure to chloride aluminum (AlCl3) on the oxidative biochemistry, proteomic profile, and morphology of the major salivary glands. Wistar male rats were exposed to 8.3 mg/kg/day of AlCl3 via intragastric gavage for 60 days. Then, the parotid and submandibular glands were subjected to biochemical assays, proteomic evaluation, and histological analysis. Al caused oxidative imbalance in both salivary glands. Dysregulation of protein expression, mainly of those related to cytoarchitecture, energy metabolism and glandular function, was detected in both salivary glands. Al also promoted histological alterations, such as acinar atrophy and an increase in parenchymal tissue. Prolonged exposure to Al, even at low doses, was able to modulate molecular alterations associated with morphological impairments in the salivary glands of rats. From this perspective, prolonged Al exposure may be a risk to exposed populations and their oral health. 相似文献
65.
Marialuisa Aragona Caterina Porcino Maria Cristina Guerrera Giuseppe Montalbano Rosaria Laur Marzio Cometa Maria Levanti Francesco Abbate Teresa Cobo Gabriel Capitelli Jos A. Vega Antonino German 《International journal of molecular sciences》2022,23(5)
The brain-derived neurotrophic factor (BDNF) was discovered in the last century, and identified as a member of the neurotrophin family. BDNF shares approximately 50% of its amino acid with other neurotrophins such as NGF, NT-3 and NT-4/5, and its linear amino acid sequences in zebrafish (Danio rerio) and human are 91% identical. BDNF functions can be mediated by two categories of receptors: p75NTR and Trk. Intriguingly, BDNF receptors were highly conserved in the process of evolution, as were the other NTs’ receptors. In this review, we update current knowledge about the distribution and functions of the BDNF-TrkB system in the sensory organs of zebrafish. In fish, particularly in zebrafish, the distribution and functions of BDNF and TrkB in the brain have been widely studied. Both components of the system, associated or segregated, are also present outside the central nervous system, especially in sensory organs including the inner ear, lateral line system, retina, taste buds and olfactory epithelium. 相似文献
66.
Cristina Jansen-Alves Karina F. Fernandes Michele M. Crizel-Cardozo Fernanda D. Krumreich Caroline D. Borges Rui C. Zambiazi 《Food and Bioprocess Technology》2018,11(7):1422-1436
Propolis presents several health benefits due to the presence of bioactive compounds, mainly phenolic compounds; however, its application in food is limited due to undesirable odor and low water solubility. The bioactive compounds are usually susceptible to degradation by exposure to light, heat, or oxygen or by interaction with other compounds, which may limit its biological activity. The study aimed the propolis extract microencapsulation using rice, pea, soybean, and ovoalbumin proteins as wall material by spray drying and to analyze their in vitro digestion. The propolis extract presented a high concentration of apigenin. Encapsulation efficiency was greater than 70%, and it was maintained the antioxidant activity of propolis (88% inhibition of DPPH for propolis extract and >?73% for the microparticles). The DSC, ATR-FTIR, and X-ray diffraction techniques confirmed the encapsulation. The microparticles showed different shapes, sizes, and physical characteristics. The microparticles encapsulated with pea protein could be used in formulations of Minas Frescal cheese due to the controlled released, whereas the other microparticles could be used in pudding formulations. 相似文献
67.
de Diego Isaac Martín Román Ignacio San Montero Javier Cano Conde Cristina Cabello Enrique 《Multimedia Tools and Applications》2019,78(13):17437-17459
Multimedia Tools and Applications - This paper presents a novel distributed intelligent video surveillance architecture based on Wireless Multimedia Sensor Networks (WMSNs). This architecture is... 相似文献
68.
Eduardo Arias‐Egido María Angeles Laguna‐Marco Cristina Piquer Roberto Boada Sofía Díaz‐Moreno 《Advanced functional materials》2019,29(10)
Amorphous and polycrystalline Sn‐doped IrO2 thin films, Ir1‐xSnxO2, are grown for the first time. Their electrical response and strength of the spin–orbit coupling are studied in order to better understand and tailor its performance as spin current detector material. These experiments prove that the resistivity of IrO2 can be tuned over several orders of magnitude by controlling the doping content in both the amorphous and the polycrystalline state. In addition, growing amorphous samples increase the resistivity, thus improving the spin current to charge current conversion. As far as the spin–orbit coupling is concerned, the system not only remains in a strong spin–orbit coupling regime but it seems to undergo a slight enhancement in the amorphous state as well as in the Sn‐doped samples. 相似文献
69.
Antimicrobial Activity of Marjoram (Origanum Majorana) Essential Oil Against the Multidrug‐Resistant Salmonella Enterica Serovar Schwarzengrund Inoculated in Vegetables from Organic Farming 下载免费PDF全文
70.
Two experiments examined the extent to which U.S. viewers' perceptions that Blacks face structural limitations to success, support for the death penalty, and culpability judgments could be influenced by exposure to racialized crime news. Participants were exposed to a majority of Black suspects, a majority of White suspects, unidentified suspects, and noncrime news stories. In addition, participants' prior news viewing was assessed. In Study 1, heavy news viewers exposed to unidentified perpetrators were less likely than heavy news viewers exposed to noncrime stories to perceive that Blacks face structural limitations to success. In addition, heavy news viewers exposed to unidentified perpetrators were more likely than heavy news viewers exposed to noncrime stories to support the death penalty. In Study 2, participants exposed to a majority of Black suspects were more likely than participants exposed to noncrime stories to find a subsequent race-unidentified criminal culpable for his offense. In addition, heavy news viewers were more likely to exhibit the above effect than light news viewers. The methodological and theoretical implications of these findings are discussed in light of chronic activation and the priming paradigm. 相似文献