Synthesis and characterization of chemically and electrochemically prepared conducting polymer/iron oxalate composites

Poly(3-octyl-thiophene) (POT) and polypyrrole (PPy) iron oxalate composites were synthesized through a post-polymerization oxidative treatment. The composite of the latter has been prepared also by electrochemical polymerization. The samples have been characterized by X-ray diffraction (XRD), impedance spectroscopy, scanning electron microscope (SEM) combined with energy dispersive X-ray (EDX) spectroscopy, Mössbauer spectroscopy, cyclic voltammetry and electrochemical quartz crystal microbalance (EQCM). In case of PPy, two peaks in the XRD spectra show the presence of iron containing composite, while with POT only the layered structure originating from the octyl side-chain interactions was modified by the composite formation. The assumption of the weakening of short- and long-range interactions was proven by the decrease in conductivity of the composite. The successful electrochemical synthesis resulted a composite of ∼5% iron content, determined by EDX. Mössbauer spectroscopy measurements evidenced a composite containing mixed valence iron oxalate doping ions, which supports the indirect EQCM data.     

On sparse matrix orderings in interior point methods

The major computational task of most interior point implementations is solving systems of equations with symmetric coefficient matrix by direct factorization methods, therefore, the performance of Cholesky-like factorizations is a critical issue. In the case of sparse and large problems the efficiency of the factorizations is closely related to the exploitation of the nonzero structure of the problem. A number of techniques were developed for fill-reducing sparse matrix orderings which make Cholesky factorizations more efficient by reducing the necessary floating point computations. We present a variant of the nested dissection algorithm incorporating special techniques that are beneficial for graph partitioning problems arising in the ordering step of interior point implementations. We illustrate the behavior of our algorithm and provide numerical results and comparisons with other sparse matrix ordering algorithms.     

Research progress of electrode suspensions of lithium-ion flow batteries

锂离子液流电池将锂离子电池的工作原理与传统液流电池的结构特点相结合,是一种正处于基础技术开发阶段的新型电化学储能电池技术,具有输出功率和储能容量彼此独立、成本较低等特点,适用于未来电网储能领域。电极悬浮液作为实现锂离子液流电池充放电功能的主体材料,其导电性能和流动性能是影响锂离子液流电池倍率特性和能量密度的重要因素。论文结合实验数据对该方向面临的主要技术问题及研究重点进行了分析,认为电极悬浮液的研究需要从导电机理、质量比容量、流变性能等方面进一步深入研究,并建立标准评价体系。     

Electrophysiological Effects of the Transient Receptor Potential Melastatin 4 Channel Inhibitor (4-Chloro-2-(2-chlorophenoxy)acetamido) Benzoic Acid (CBA) in Canine Left Ventricular Cardiomyocytes

Transient receptor potential melastatin 4 (TRPM4) plays an important role in many tissues, including pacemaker and conductive tissues of the heart, but much less is known about its electrophysiological role in ventricular myocytes. Our earlier results showed the lack of selectivity of 9-phenanthrol, so CBA ((4-chloro-2-(2-chlorophenoxy)acetamido) benzoic acid) was chosen as a new, potentially selective inhibitor. Goal: Our aim was to elucidate the effect and selectivity of CBA in canine left ventricular cardiomyocytes and to study the expression of TRPM4 in the canine heart. Experiments were carried out in enzymatically isolated canine left ventricular cardiomyocytes. Ionic currents were recorded with an action potential (AP) voltage-clamp technique in whole-cell configuration at 37 °C. An amount of 10 mM BAPTA was used in the pipette solution to exclude the potential activation of TRPM4 channels. AP was recorded with conventional sharp microelectrodes. CBA was used in 10 µM concentrations. Expression of TRPM4 protein in the heart was studied by Western blot. TRPM4 protein was expressed in the wall of all four chambers of the canine heart as well as in samples prepared from isolated left ventricular cells. CBA induced an approximately 9% reduction in AP duration measured at 75% and 90% of repolarization and decreased the short-term variability of APD₉₀. Moreover, AP amplitude was increased and the maximal rates of phase 0 and 1 were reduced by the drug. In AP clamp measurements, CBA-sensitive current contained a short, early outward and mainly a long, inward current. Transient outward potassium current (I_to) and late sodium current (I_Na,L) were reduced by approximately 20% and 47%, respectively, in the presence of CBA, while L-type calcium and inward rectifier potassium currents were not affected. These effects of CBA were largely reversible upon washout. Based on our results, the CBA induced reduction of phase-1 slope and the slight increase of AP amplitude could have been due to the inhibition of I_to. The tendency for AP shortening can be explained by the inhibition of inward currents seen in AP-clamp recordings during the plateau phase. This inward current reduced by CBA is possibly I_Na,L, therefore, CBA is not entirely selective for TRPM4 channels. As a consequence, similarly to 9-phenanthrol, it cannot be used to test the contribution of TRPM4 channels to cardiac electrophysiology in ventricular cells, or at least caution must be applied.     

Therapeutic Window of Ligand‐Free Silver Nanoparticles in Agar‐Embedded and Colloidal State: In Vitro Bactericidal Effects and Cytotoxicity

The inhibition of bacterial growth through effective non‐toxic antimicrobial substances is of great importance for the prevention and therapy of implant infections in various medical disciplines. For the evaluation of a therapeutic window of silver nanoparticles (AgNPs), their bactericidal properties were tested in agar composites and colloids on four medical relevant bacteria. Therefore, we produced AgNPs using high‐power nanosecond laser ablation in water showing a log‐normal particle diameter distribution centered at 17 nm. Bacteria were incubated with AgNP concentrations ranging from 5 to 70 µg · mL^?1 and the growth rate was recorded. Additionally, cytotoxic effects of AgNPs on human gingival fibroblasts were examined. The experiments demonstrated that laser‐synthesized AgNPs resulted in a significant bacterial growth inhibition of more than 80% at the indicated concentrations in a solid agar model (Pseudomonas aeruginosa 10 µg · mL^?1, Streptococcus salivarius 10 µg · mL^?1, Escherichia coli 20 µg · mL^?1, Staphylococcus aureus 70 µg · mL^?1). In a planktonic bacteria model, the growth of the tested bacteria was significantly delayed by the addition of AgNPs at a concentration of 35 µg · mL^?1. The cytotoxic assays showed limited adverse effects on human fibroblasts at concentrations of less than 20 µg · mL^?1. The present study illustrates the strong antibacterial effects of ligand‐free, laser‐generated AgNPs that exhibit moderate cytotoxic effects, resulting in a therapeutically applicable concentration of AgNPs for medical purposes between 10 and 20 µg · mL^?1.     

Exploration–exploitation tradeoff using variance estimates in multi-armed bandits

Algorithms based on upper confidence bounds for balancing exploration and exploitation are gaining popularity since they are easy to implement, efficient and effective. This paper considers a variant of the basic algorithm for the stochastic, multi-armed bandit problem that takes into account the empirical variance of the different arms. In earlier experimental works, such algorithms were found to outperform the competing algorithms. We provide the first analysis of the expected regret for such algorithms. As expected, our results show that the algorithm that uses the variance estimates has a major advantage over its alternatives that do not use such estimates provided that the variances of the payoffs of the suboptimal arms are low. We also prove that the regret concentrates only at a polynomial rate. This holds for all the upper confidence bound based algorithms and for all bandit problems except those special ones where with probability one the payoff obtained by pulling the optimal arm is larger than the expected payoff for the second best arm. Hence, although upper confidence bound bandit algorithms achieve logarithmic expected regret rates, they might not be suitable for a risk-averse decision maker. We illustrate some of the results by computer simulations.     

High performance lattice reduction on heterogeneous computing platform

The lattice reduction (LR) technique has become very important in many engineering fields. However, its high complexity makes difficult its use in real-time applications, especially in applications that deal with large matrices. As a solution, the modified block LLL (MB-LLL) algorithm was introduced, where several levels of parallelism were exploited: (a) fine-grained parallelism was achieved through the cost-reduced all-swap LLL (CR-AS-LLL) algorithm introduced together with the MB-LLL by Józsa et al. (Proceedings of the tenth international symposium on wireless communication systems, 2013) and (b) coarse-grained parallelism was achieved by applying the block-reduction concept presented by Wetzel (Algorithmic number theory. Springer, New York, pp 323–337, 1998). In this paper, we present the cost-reduced MB-LLL (CR-MB-LLL) algorithm, which allows to significantly reduce the computational complexity of the MB-LLL by allowing the relaxation of the first LLL condition while executing the LR of submatrices, resulting in the delay of the Gram–Schmidt coefficients update and by using less costly procedures during the boundary checks. The effects of complexity reduction and implementation details are analyzed and discussed for several architectures. A mapping of the CR-MB-LLL on a heterogeneous platform is proposed and it is compared with implementations running on a dynamic parallelism enabled GPU and a multi-core CPU. The mapping on the architecture proposed allows a dynamic scheduling of kernels where the overhead introduced is hidden by the use of several CUDA streams. Results show that the execution time of the CR-MB-LLL algorithm on the heterogeneous platform outperforms the multi-core CPU and it is more efficient than the CR-AS-LLL algorithm in case of large matrices.     

Oscillating hydrogenase reaction

The hydrogenase-catalyzed oxidation of H₂ includes an autocatalytic step in the reaction cycle. The reaction also exhibits different pH dependence in the H₂ oxidation and in the proton reduction directions. This is not only due to the pH titration of the amino acid side chains as protons are also either the substrates or the products of the reaction. Utilizing the autocatalytic nature of the hydrogenase reaction and the multiple roles of protons therein, together with appropriate limitation of the substrate (gaseous H₂) supply, oscillations can be induced in the system. The reaction oscillates both in space and in time, and can last for days with decreasing frequency until reaching chemical equilibrium. Of all biological oscillating systems described so far, this one is the simplest in that it has the fewest biological components.     

Open tubular heterogeneous enzyme reactors: analysis of a theoretical model

A novel reactor, made by coating the inner wall of tubes with an enzyme resin layer, was mathematically modeled. Michaelis-Menten kinetics, laminar flow and isothermal conditions were assumed and substrate diffusion in both the streaming fluid and the catalyst annulus was taken into account. The behavior and effectiveness of the reactor are discussed in terms of characteristic dimensionless groups including the reactor coordinate which is inversely proportional to the average overall rate at a given conversion. An experimental criterion is given for the absence of all diffusional effects which may mask kinetic data. The calculated reactor coordinates can be approximated by the sun of their limiting values pertaining to the kinetically controlled and bulk diffusion controlled reaction thus permitting a simplified treatment of the reactor data and of problems of reactor design. Conditions for extracting kinetic parameters from experimental data are discussed.