Client-specific counselor self-efficacy in novice counselors: Relation to perceptions of session quality.

Two versions of a counselor self-efficacy (CSE) measure were administered to 110 prepracticum counselors: a general version, assessing perceived capability to perform basic helping skills and manage the session process with clients generally; and a client-specific version, tapping capability to perform the same behaviors with a specific, current client. Client-specific CSE was found to (a) relate moderately to strongly with general CSE over the course of four counseling sessions, (b) increase significantly over sessions, and (c) account for unique variance in counselors' evaluations of the quality of their sessions. Although it was not a useful direct predictor of clients' session ratings, higher client-specific CSE was associated with greater congruence between counselors' and clients' perceptions of session quality. Implications for further research and training are considered. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Applications of infrared spectroscopy to customer problems in the vinyl industry

Identifying polymers, additives and contaminants presents unique challenges. Fourier transform infrared spectroscopy (FT-IR) coupled with spectral searching techniques is a very efficient and powerful methodology to answer most identification questions. It is based on the unique IR spectrum characteristic of each compound. This paper outlines procedures employed in the identification of typical PVC formulations, starting with the resin and proceeding to impact modifier, process aids, lubricants, stabilizer system and fillers. The emphasis is on FT-IR microscopy as the most versatile approach requiring small samples and minimal to no sample preparation. Other commonly used FT-IR techniques are also outlined. Wet separation protocols, applicability and limitations as related to FT-IR analysis are discussed. Typical examples include vinyl siding, packaging and bottle formulations as well as contaminants often encountered in these formulations and raw materials.     

Chemistry of small organic molecules on snow grains: the applicability of artificial snow for environmental studies

The utilization of artificial snow for environmentally relevant (photo)chemical studies was systematically investigated. Contaminated snow samples were prepared by various methods: by shock freezing of the aqueous solutions sprayed into liquid nitrogen or inside a large walk-in cold chamber at -35 °C, or by adsorption of gaseous contaminants on the surface of artificially prepared pure or natural urban snow. The specific surface area of artificial snow grains produced in liquid nitrogen was determined using valerophenone photochemistry (400-440 cm(2) g(-1)) to estimate the surface coverage by small hydrophobic organic contaminants. The dynamics of recombination/dissociation (cage effect) of benzyl radical pairs, photochemically produced from 4-methyldibenzyl ketone on the snow surface, was investigated. The initial ketone loading, c = 10(-6)-10(-8) mol kg(-1), only about 1-2 orders of magnitude higher than the contaminant concentrations commonly found in nature, was already well below monolayer coverage. We found that the efficiency of out-of-cage reactions decreased at much higher temperatures than those previously determined for frozen solutions; however, the cage effect was essentially the same no matter what technique of snow production or ketone deposition/uptake was used, including the experiments with collected natural snow. The experimental observation that the contaminant molecules are initially self-associated even at the lowest concentrations was supported by DFT calculations. We conclude that, contrary to frozen aqueous solutions, in which the impurities reside in a 3D cage (micropocket), contaminant molecules located on the artificial snow grain surface at low concentrations can be visualized in terms of a 2D cage. Artificial snow thus represents a readily available study matrix that can be used to emulate the natural chemical processes of trace contaminants occurring in natural snow.     

Microfluidic transendothelial electrical resistance measurement device that enables blood flow and postgrowth experiments

Transendothelial electronic resistance (TEER) measurements are performed across a cell layer immobilized on a microfluidic device that also enables the cell layer to interact with a flowing stream of red blood cells (RBCs). A bipolar pulsed square wave potential is applied across a monolayer of bovine pulmonary artery endothelial cells, and the resulting current response is measured and integrated. The overall impedance of the cell layer provides an indicator of cell layer integrity. After cell seeding on the device, a decrease in TEER signal from 22.3 ± 1.6 μC to 3.5 ± 0.4 μC (corresponding to a resistance of 40.9 ± 2.9 Ω·cm(2) to 259.1 ± 27.4 Ω·cm(2)) was observed after 8 h of cell growth. Intracellular nitric oxide (NO) production by the immobilized endothelial cells that had reached confluence was 34% higher than those cells that had not reached confluence, as indicated by the integrated TEER system. Importantly, this NO production by the confluent endothelium was stimulated by ATP released from RBCs flowing under the endothelial cells. In this construct, the described microfluidic device enables both a TEER-based evaluation of cell layer integrity and molecularly communicated interactions of these cells with a flowing stream of blood components.     

Diagnosing Czech Patients with Inherited Platelet Disorders

A single-center study was conducted on 120 patients with inherited disorders of primary hemostasis followed at our hematological center. These patients presented a variety of bleeding symptoms; however, they had no definitive diagnosis. Establishing a diagnosis has consequences for the investigation of probands in families and for treatment management; therefore, we aimed to improve the diagnosis rate in these patients by implementing advanced diagnostic methods. According to the accepted international guidelines at the time of study, we investigated platelet morphology, platelet function assay, light-transmission aggregometry, and flow cytometry. Using only these methods, we were unable to make a definitive diagnosis for most of our patients. However, next-generation sequencing (NGS), which was applied in 31 patients, allowed us to establish definitive diagnoses in six cases (variants in ANKRD26, ITGA2B, and F8) and helped us to identify suspected variants (NBEAL2, F2, BLOC1S6, AP3D1, GP1BB, ANO6, CD36, and ITGB3) and new suspected variants (GFI1B, FGA, GP1BA, and ITGA2B) in 11 patients. The role of NGS in patients with suspicious bleeding symptoms is growing and it changes the diagnostic algorithm. The greatest disadvantage of NGS, aside from the cost, is the occurrence of gene variants of uncertain significance.     

Therapeutic Efficacy and Outcomes of Remdesivir versus Remdesivir with Tocilizumab in Severe SARS-CoV-2 Infection

The infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) generated many challenges to find an effective drug combination for hospitalized patients with severe forms of coronavirus disease 2019 (COVID-19) pneumonia. We conducted a retrospective cohort study, including 182 patients with severe COVID-19 pneumonia hospitalized between March and October 2021 in a Pneumology Hospital from Cluj-Napoca, Romania. Among patients treated with standard of care, 100 patients received remdesivir (R group) and 82 patients received the combination of remdesivir plus tocilizumab (RT group). We compared the clinical outcomes, the inflammatory markers, superinfections, oxygen requirement, intensive care unit (ICU) admission and mortality rate before drug administration and 7 days after in R group and RT group. Borg score and oxygen support showed an improvement in the R group (p < 0.005). Neutrophiles, C-reactive protein (CRP) and serum ferritin levels decreased significantly in RT group but with a higher rate of superinfection in this group. ICU admission and death did not differ significantly between groups. The combination of remdesivir plus tocilizumab led to a significantly improvement in the inflammatory markers and a decrease in the oxygen requirement. Although the superinfection rate was higher in RT group than in R group, no significant difference was found in the ICU admission and mortality rate between the groups.     

Model Identification and Control of Electromagnetic Actuation in Continuous Casting Process With Improved Quality

This paper presents an original theoretical framework to model steel material properties in continuous casting line process. Specific properties arising from non-Newtonian dynamics are herein used to indicate the natural convergence of distributed parameter systems to fractional order transfer function models. Data driven identification from a real continuous casting line is used to identify model of the electromagnetic actuator device to control flow velocity of liquid steel. To ensure product specifications, a fractional order control is designed and validated on the system. A projection of the closed loop performance onto the quality assessment at end production line is also given in this paper.