Integration of virtual and real environments for engineering service-oriented manufacturing systems

Engineering frameworks are currently required to support the easy, low-cost, modular and integrated development of manufacturing systems addressing the emergent requirements of re-configurability, responsiveness and robustness. This paper discusses the integration of 2D/3D digital software tools with Petri net based service-oriented frameworks to allow the design, configuration, analysis, validation, simulation, monitoring and control of manufacturing systems in a virtual environment and its posterior smooth migration into the real ??physical?? environment. An experimental case study was implemented to validate the proposed concepts, using the Continuum platform to design, compose, analyze, validate and simulate the Petri nets based service-oriented manufacturing control system, and the Delmia Automation^TM software suite to support the rapid prototyping and the easy simulation of the designed control solution. The experimental results prove several aspects of the proposed approach, notably the smooth migration between the design and the operation phases, one of the main objectives of the work.     

Self-Organising Maps: A new way to screen the level of satisfaction of dialysis patients

Evaluation of patient satisfaction has become an important indicator for assessing health care quality. Fresenius Medical Care (FME) as a global provider of dialysis services through its NephroCare network has a strong interest in monitoring patient satisfaction.The aim of the paper is to test and validate a methodology for detecting a residual area of low satisfaction in dialysis patients.The FME Patient Satisfaction Programme questionnaire was distributed to haemodialysis (HD) patients treated in 335 centers of its network. It contained 79 questions covering various satisfaction aspects regarding Dialysis Unit, Dialysis Arrangement, Nurses, Doctors, etc.To analyse the data provided by the questionnaire, the Self-Organising Map (SOM) method was used. SOM is a neural network model for clustering and projecting high-dimensional data into a low-dimensional space, preserving topological relationships of original high-dimensional data spaces.10,632 HD patients completed the questionnaire. Mean age was 63.05 ± 14.93 years with 56.69% males. Response rate was 66%. Overall level of satisfaction was 1.99 (range from ?3 to +3). On average patients were very satisfied with all issues. Nevertheless, a group of patients, around 60 years old, balanced gender ratio, whose level of satisfaction was lower than 1, were highlighted.In the NephroCare clinics patient satisfaction with service is rather high. While traditional analysis usually stops here, the SOM method allows identification of areas of potential improvement for specific patient groups.     

AE energy analysis on concrete bridge beams

Concrete bridges were built originally to have a cost-effective maintenance free life of 120 years, however both in Europe and the USA this has proved to the totally optimistic. Concrete bridges require major maintenance after twenty to thirty years in order to extend their life The Acoustic Emission technique has been successfully applied to monitor and provide information on the safety of concrete bridges, but the processing of the AE data is often not trivial. This paper proposes a method of AE analysis to assess concrete bridges based on the fact that the AE energy is one of the effective parameters to evaluate the damage of a concrete structure. Results from experiments on concrete beams with different design and loading configurations are presented. It is argued that in some cases the state of damage of bridge beams can be estimated from the AE energy analysis when approximately 45% of the ultimate load has been applied.     

Inversion of a radiative transfer model with hyperspectral observations for LAI mapping in poplar plantations

The potential of radiative transfer modelling and inversion techniques for operational uses is investigated in order to retrieve leaf area index in a poplar plantation. The 1-D bidirectional canopy reflectance model SAIL, coupled with the leaf optical properties model PROSPECT, was inverted with hyperspectral airborne DAIS data by means of an iterative method. The root mean square error in LAI estimation was determined against in situ measurements in order to evaluate the impact of different inversion strategies on the LAI retrieval accuracy. These included the selection of an optimal spectral sampling set, the exploitation of prior knowledge in the inversion process and the use of multiview angle data. We claim that the best configuration is achieved by exploiting multiview DAIS data and prior knowledge information about the model variables (RMSE of 0.39 m² m⁻²). It is also shown that the use of prior knowledge and the selection of a limited number of bands forming the optimal spectral sampling are instrumental in increasing the accuracy of the inversion process. Our analysis confirms the operational potential of model inversion for biophysical parameter retrieval.     

Multipurpose modular experimental station for the DiProI beamline of Fermi@Elettra free electron laser

We present a compact modular apparatus with a flexible design that will be operated at the DiProI beamline of the Fermi@Elettra free electron laser (FEL) for performing static and time-resolved coherent diffraction imaging experiments, taking advantage of the full coherence and variable polarization of the short seeded FEL pulses. The apparatus has been assembled and the potential of the experimental setup is demonstrated by commissioning tests with coherent synchrotron radiation. This multipurpose experimental station will be open to general users after installation at the Fermi@Elettra free electron laser in 2011.     

Proteins as biomarkers of oxidative/nitrosative stress in diseases: the contribution of redox proteomics

Reactive oxygen species (ROS) and reactive nitrogen species (RNS) contribute to the pathogenesis and/or progression of several human diseases. Proteins are important molecular signposts of oxidative/nitrosative damage. However, it is generally unresolved whether the presence of oxidatively/nitrosatively modified proteins has a causal role or simply reflects secondary epiphenomena. Only direct identification and characterization of the modified protein(s) in a given pathophysiological condition can decipher the potential roles played by ROS/RNS-induced protein modifications. During the last few years, mass spectrometry (MS)-based technologies have contributed in a significant way to foster a better understanding of disease processes. The study of oxidative/nitrosative modifications, investigated by redox proteomics, is contributing to establish a relationship between pathological hallmarks of disease and protein structural and functional abnormalities. MS-based technologies promise a contribution in a new era of molecular medicine, especially in the discovery of diagnostic biomarkers of oxidative/nitrosative stress, enabling early detection of diseases. Indeed, identification and characterization of oxidatively/nitrosatively modified proteins in human diseases has just begun.     

Discovery of an Allosteric Ligand Binding Site in SMYD3 Lysine Methyltransferase

SMYD3 is a multifunctional epigenetic enzyme with lysine methyltransferase activity and various interaction partners. It is implicated in the pathophysiology of cancers but with an unclear mechanism. To discover tool compounds for clarifying its biochemistry and potential as a therapeutic target, a set of drug-like compounds was screened in a biosensor-based competition assay. Diperodon was identified as an allosteric ligand; its R and S enantiomers were isolated, and their affinities to SMYD3 were determined (K_D=42 and 84 μM, respectively). Co-crystallization revealed that both enantiomers bind to a previously unidentified allosteric site in the C-terminal protein binding domain, consistent with its weak inhibitory effect. No competition between diperodon and HSP90 (a known SMYD3 interaction partner) was observed although SMYD3–HSP90 binding was confirmed (K_D=13 μM). Diperodon clearly represents a novel starting point for the design of tool compounds interacting with a druggable allosteric site, suitable for the exploration of noncatalytic SMYD3 functions and therapeutics with new mechanisms of action.     

Power Efficient SDR Implementation of IEEE 802.11a/p Physical Layer

Software defined physical layer modems can be considered the new trend in the field of communications. Differently from dedicated hardware, software can be easily modified to implement a large variety of standards on the same platform. The use of software can significantly reduce development costs, but generally comes at the price of an increase in silicon area and power consumption. For different reasons, this price is something that is not always convenient or even possible to pay, as in the case of low-cost ICs implementing a single waveform, or even multi-mode modems embedding legacy IPs already available in hardware. In particular, power consumption overhead can be prohibitive for mobile terminals or in general for battery-powered devices. The very first challenge for a computing fabric to be competitive is to find and implement the right trade-off between flexibility and performance. This was the guideline for the design of the Block Processing Engine (BPE), a template architecture conceived for power-efficient baseband processing. The BPE core feature is a mixed-grain instruction set balancing general-purpose fine-grain instructions with more specific coarse-grain instructions wrapping custom hardware modules. To further limit the power consumption, the BPE also implements instruction-pipelining, variable-size SIMD and multi-task support. To prove the efficiency of such an approach, a dual-mode IEEE 802.11a/p receiver has been implemented.     

Influence of the Tunnel Shape on Shotcrete Lining Stresses

Tunnel excavation is frequently carried out in rock masses by the drill and blast method and the final shape of the tunnel boundary can be irregular due to overbreaks. In order to investigate the effects of overbreaks a study of the effect of tunnel boundary irregularity has been carried out. This is done developing a computational tool able to take into account fuzzy variables (i.e., thickness of the beams of the bedded spring approach used for the model). The obtained results show that irregularity effects should be considered when a shotcrete lining is used as the final tunnel lining (for the case where the tunneling procedure does not permit a smooth surface to be obtained). This is crucial to obtain a durable lining.