Cyclic variations in the permeability of the cell wall of Saccharomyces cerevisiae.

To study cell-cycle-related variations in wall permeability of Saccharomyces cerevisiae, two approaches were used. First, an asynchronous culture was fractionated by centrifugal elutriation into subpopulations containing cells of increasing size. The subpopulations represented different stages of the cell cycle as judged by light microscopy. Cell wall porosity increased when these subpopulations became enriched with budded cells. Secondly, synchronous cultures were obtained by releasing MATa cells from alpha-factor induced G1-arrest. These cultures grew synchronously for at least two generations. The cell wall porosity increased sharply in these cultures, shortly before buds became visible and was maximal during the initial stages of bud growth. It decreased in cells which had completed nuclear migration and before abscission of the bud had occurred. The porosity reached its lowest value during abscission and in unbudded cells. We examined the incorporation of mannoproteins into the wall during the cell cycle. SDS-extractable mannoproteins were incorporated continuously. However, the incorporation of glucanase-extractable mannoproteins, which are known to affect cell wall porosity, showed cyclic oscillations and reached its maximum after nuclear migration. This coincided with a rapid decrease in cell wall porosity, indicating that glucanase-extractable mannoproteins might contribute to this decrease.     

Effects of e-enforcement in the Netherlands

E-enforcement, or electronic enforcement, is the use of electronic tools in law enforcement. We shall distinguish between the first and second generations of e-enforcement. The first generation of e-enforcement tools provided means of "automated enforcement". This article is based on our research into the effects of two forms of second generation e-enforcement: the digital tachograph and 'Weigh in Motion' (WIM). The digital tachograph replaces the analog tachograph in all heavy goods vehicles within the European Union. The equipment registers drivers' driving and rest times. Weigh in Motion with Video (WIM-Vid) is a system involving sensors in the road and cameras, which detect and register overloading of heavy goods vehicles. The WIM-Vid system was developed and implemented in the Netherlands and is currently attracting international attention. We compare the situation prior to the introduction of these e-enforcement measures to the situation after the introduction of e-enforcement.     

STUDY ON RESIDUES OF ~（14）C－FENITROTHION IN MODEL RICE－FISH ECOSYSTEM AND FIELD RICE－FISH ECOSYSTEM

ＳＴＵＤＹＯＮＲＥＳＩＤＵＥＳＯＦ~（１４）Ｃ－ＦＥＮＩＴＲＯＴＨＩＯＮＩＮＭＯＤＥＬＲＩＣＥ－ＦＩＳＨＥＣＯＳＹＳＴＥＭＡＮＤＦＩＥＬＤＲＩＣＥ－ＦＩＳＨＥＣＯＳＹＳＴＥＭＺｈａｎｇＺｈｏｎｇｌｉａｎｇ（张仲良）；ＷａｎｇＨｕａｘｉｎ（王化新）；Ｇ?..     

X射线聚束透镜原理及其应用

本文阐述了一种新型的Ｘ射线聚束装置─—Ｘ射线透镜的聚束原理。给出了透镜的基本设计方法，并对它应用于Ｘ射线光刻研究领域的前景作了展望。     

用于重离子核反应实验测量的纵向电场气体电离室

本文报道了一种新型的大动态范围，多叠层探测器系统组件之一的纵向电场气体电离室的构造，性能和在中能得离子核反应实验中的应用。     

The application of semantic classification trees to naturallanguage understanding

This article describes a new method for building a natural language understanding (NLU) system, in which the system's rules are learnt automatically from training data. The method has been applied to design of a speech understanding (SU) system. Designers of such systems rely increasingly on robust matchers to perform the task of extracting meaning from one or several word sequence hypotheses generated by a speech recognizer. We describe a new data structure, the semantic classification tree (SCT), that learns semantic rules from training data and can be a building block for robust matchers for NLU tasks. By reducing the need for handcoding and debugging a large number of rules, this approach facilitates rapid construction of an NLU system. In the case of an SU system, the rules learned by an SCT are highly resistant to errors by the speaker or by the speech recognizer because they depend on a small number of words in each utterance. Our work shows that semantic rules can be learned automatically from training data, yielding successful NLU for a realistic application     

A multi-purpose CMOS sensor interface for low-power applications

A dedicated low-power CMOS transponder microchip is presented as part of a novel telemetry implant for biomedical applications. This mixed analog-digital circuit contains an identification code and collects information on physiological parameters, i.e., body temperature and physical activity, and on the status of the battery. To minimize the amount of data to be transmitted, a dedicated signal processing algorithm is embedded within its circuitry. All telemetry functions (encoding, modulation, generation of the carrier) are implemented on the integrated circuit. Emphasis is on a high degree of flexibility towards sensor inputs and internal data management, extreme miniaturization, and low-power consumption to allow a long implantation lifetime     

New insights into the relation between channel hot carrier degradation and oxide breakdown short channel nMOSFETs

In this letter, we report new findings in the relation between channel hot-carrier (CHC) degradation and gate-oxide breakdown (BD) in short-channel nMOSFETS biased at V/sub G/>V/sub D/. We observe that the time-to-BD is strongly reduced in the hot carrier regime and that although the channel hot-electron injection into the oxide occurs mainly at the drain side, stress-induced leakage current (SILC) generation and oxide BD always occur at the source side. The results of these measurements indicate that not solely the energy of the injected electrons but also the oxide electric field is determinant in the oxide BD process.