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21.
Brefeldin A (BFA) has been reported to cause disassembly of the Golgi. We have used three-dimensional (3-D) high-resolution scanning electron microscopy (HRSEM) to investigate these effects in human skin fibroblast cells. The spontaneous reassembly during prolonged exposure to BFA and some effects of forskolin were observed. A BFA concentration of 5μg/ml caused Golgi complexes to become vesicular, resulting in a progressive decrease in the size of the Golgi. Morphologic changes were visible within 2 min of BFA incubation, and by 30 min no identifiable Golgi could be found. Spontaneous reassembly of the Golgi apparatus upon the removal of the BFA or with continued long-term exposure with BFA could not be confirmed. Preliminary experiments with forskolin were not effective in reversing or inhibiting the effects of BFA in human fibroblast cells grown in culture. This inability for spontaneous reassembly and nonreversal by forskolin may reflect a differential effect of BFA in various cell types. HRSEM has proven to be useful for observing 3-D morphologic effects of BFA in Golgi. 相似文献
22.
Levin Harvey S.; Mendelsohn Dianne; Lilly Matthew A.; Fletcher Jack M.; Culhane Kathleen A.; Chapman Sandra B.; Harward Harriet; Kusnerik Lori; Bruce Derek; Eisenberg Howard M. 《Canadian Metallurgical Quarterly》1994,8(2):171
To investigate the relationship of severity of pediatric closed head injury (CHI), the contribution of frontal lobe lesions, and age at testing (6–10 yr olds vs 11–15 yr olds) to cognitive deficit, 134 head-injured patients were given the Tower of London (TOL) task and underwent magnetic resonance imaging. 89 normal controls were given the TOL for comparison. Severity of CHI and age at testing were strongly related to cognitive performance on the TOL, including the frequency of breaking the rules. Volume of frontal lobe lesion (but not extrafrontal lesion) contributed to the prediction of perfomance on the TOL even after severity of injury was taken into account. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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David C. Apperley Robin K. Harris Gregory L. Marshall Derek P. Thompson 《Journal of the American Ceramic Society》1991,74(4):777-782
The 29 Si MAS NMR spectra of the 2H, 4H, 6H, and 3C polytypes of silicon carbide are presented. An attempt is made to correlate differences in the chemical shifts with local atomic environment. The results of the analysis of the spectra of pure polytypes are used as a basic for the interpretation of the spectra of mixed polytypes and a discussion of the crystallinity and impurity levels of different samples. Carbon-13 chemical shifts obtained from spectra of the same polytypes are also tabulated. 相似文献
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Gary E. Wilson Derek C. Wadsworth Barry G. Miller Lewis Lommers Peter Kroeger Mitchell Olszewski Robert Kochan Ken Liebelt 《Nuclear Engineering and Design》1992,136(3)
The Modular High Temperature Gas-Cooled Reactor (MHTGR) is a candidate design for new production and commercial power nuclear reactors. The MHTGR has inherent safety features including: (1) passive shutdown and decay heat removal, (2) reduced requirements for operator intervention, thereby reducing sensitivity to operator error, and (3) long time intervals for corrective action. In support of the Department of Energy's (DOE) initial development of the design, the authors have completed an evaluation of the thermal-hydraulic phenomena that will dominate the plant response during representative normal, off-normal and accident conditions. Phenomena having significance to the plant behavior have been identified, and ranked with respect to their relative importance in satisfying operational, investment and/or safety criteria. The resulting information provides the basis for evaluating the applicability of existing computer codes, and defines the requirements for the development of new codes, for thermal-hydraulic systems analysis. The phenomena-based requirements also support the quality assurance related verification and validation of these codes. This paper briefly describes the methodology employed, and gives illustrative examples of the resulting requirements. References are cited for reports that document the full body of requirements and provide additional information for the methodology. 相似文献
27.
Since most end-of-life electronics equipment contain hazardous materials such as lead solder alloys or lead-impregnated glass, it is important to divert them from landfills. For end-of-life products that are not repairable and do not contain reusable parts, bulk recycling is an alternative to recover base materials. In this paper, we contrast production and recycling planning and distribution decisions, activities, and costs. We reveal that while a traditional production facility connects suppliers and customers, a recycling facility connects both "input" and "output" customers. As a result, decisions in short-term bulk recycling planning include what products to accept, what products to process and reprocess, and what products to carry in inventory. Many recyclers set prices to receive "input" based on experience. For various prices to receive "input", we use a new analytical model to investigate the sensitivity of the short-term bulk recycling planning decisions in products from two different sources: industrial returns versus residential returns. The results of the case study show that different decisions are recommended for the industrial returns versus the residential returns when the total quantity of the incoming products is equal. 相似文献
28.
Les Hatton Andy Wright Stuart Smith Gregg Parkes Paddy Bennett Robert Laws 《Software》1988,18(4):301-329
The portability of software has become a major commercial issue in recent times. Such portability does not come easily, as few if any computer languages are really portable in practice. An additional complicating factor, especially in the commercial environment, is that the resulting software must be efficient. This paper describes the design and successful implementation of a 500,000+ line portable FORTRAN 77 package for the processing of seismic data. The package exhibits demonstrably high efficiency on a wide variety of machines from minicomputers to the largest supercomputers. Experiences gained during this exercise throw much light on the integration of the various thought processes which occur during the software engineering cycle, especially the notion of locality. 相似文献
29.
Iron(III) oxide tablets were electrolytically reduced to iron in molten sodium hydroxide at 530 °C and recovered to produce
iron with 2 wt.% oxygen suitable for re-melting. The cell was operated at 1.7 V and an inert nickel anode was used. The thermodynamics
and mechanism of the process was also investigated. By controlling the activity of sodium oxide in the melt, the cell could
be operated below the decomposition voltage of the electrolyte with the net sequence of events being the ionization of oxygen,
its subsequent transport to the anode and discharge leaving behind iron at the cathode. A reduction time of 1 h was achieved
for a 1 g oxide tablet (close to the theoretical reduction time predicted by Faraday’s laws) at a current density of 520 mA cm−2 with iron phase yields of ∼90 wt.%. The energy consumption was 2.8 kWh kg−1. 相似文献
30.