全文获取类型
收费全文 | 1616篇 |
免费 | 32篇 |
国内免费 | 3篇 |
专业分类
电工技术 | 23篇 |
综合类 | 2篇 |
化学工业 | 168篇 |
金属工艺 | 19篇 |
机械仪表 | 12篇 |
建筑科学 | 23篇 |
矿业工程 | 2篇 |
能源动力 | 21篇 |
轻工业 | 97篇 |
水利工程 | 9篇 |
石油天然气 | 4篇 |
无线电 | 107篇 |
一般工业技术 | 99篇 |
冶金工业 | 963篇 |
原子能技术 | 6篇 |
自动化技术 | 96篇 |
出版年
2023年 | 3篇 |
2022年 | 9篇 |
2021年 | 9篇 |
2020年 | 7篇 |
2019年 | 18篇 |
2018年 | 21篇 |
2017年 | 20篇 |
2016年 | 8篇 |
2015年 | 18篇 |
2014年 | 31篇 |
2013年 | 47篇 |
2012年 | 23篇 |
2011年 | 32篇 |
2010年 | 26篇 |
2009年 | 46篇 |
2008年 | 27篇 |
2007年 | 25篇 |
2006年 | 26篇 |
2005年 | 21篇 |
2004年 | 21篇 |
2003年 | 25篇 |
2002年 | 36篇 |
2001年 | 31篇 |
2000年 | 16篇 |
1999年 | 38篇 |
1998年 | 338篇 |
1997年 | 195篇 |
1996年 | 117篇 |
1995年 | 59篇 |
1994年 | 55篇 |
1993年 | 64篇 |
1992年 | 12篇 |
1991年 | 13篇 |
1990年 | 10篇 |
1989年 | 19篇 |
1988年 | 13篇 |
1987年 | 9篇 |
1986年 | 7篇 |
1985年 | 7篇 |
1984年 | 4篇 |
1983年 | 4篇 |
1982年 | 9篇 |
1981年 | 14篇 |
1980年 | 6篇 |
1979年 | 6篇 |
1978年 | 4篇 |
1977年 | 34篇 |
1976年 | 62篇 |
1975年 | 3篇 |
1972年 | 2篇 |
排序方式: 共有1651条查询结果,搜索用时 15 毫秒
31.
32.
Primary cultures of neonatal rat aortic smooth muscle cells inoculated at high densities (1 X 10(6) cells/25 cm2 Falcon flask) with adequate nutrient media and pH control grow rapidly and form multilayers of cells with typical "hill and valley" organization. After 10 days growth insoluble elastin formation could be visualized by phase contrast microscopy as small particles which grew rapidly to become larger irregular refractile aggregates and later coalesced to form larger aggregates and small fibres. With light and electronmicroscopy, elastin was the predominant matrix protein formed, with the "hill regions" of cultures containing abundant elastin aggregates and some collagen. In 2-week-old cultures differentiation could be observed within the cell multilayer. The older deeper cells contained more protein synthesis organelles and myofilaments and were in close association with large often coalescing elastin aggregates; compared to younger more superficial cells which contained more free polyribosomes less myofilaments, and were associated with fewer and small elastin aggregates. In older cultures this differentiation was not apparent; the cells contained many myofilaments, dense bodies, and lysosomes. Elastin aggregates and newly formed elastic fibres were abundant in the matrix. Quantitative analysis of insoluble elastin formation in the cell layer during the 4-week culture period indicated continuous biosynthesis and deposition which paralleled that of desmosine formation. Amino-acid analysis of a hot alkali insoluble residue (regarded as elastin) from 30-day-old cultures gave a profile identical with neonatal rat aortic elastin in vivo. Insoluble collagen formation in the cell layer tended to plateau after the log phase of growth was completed (10 days). Proteoglycans were found predominantly in the supernatant media. Glycosaminoglycan analysis revealed a profile of dermatan sulphate (32%), chondroitin 4-sulphate (43%), keratan and heparan sulphate (30%), with only a trace of hyaluronic acid. This study indicates that primary cultures of neonatal rat aortic smooth muscle cells remain differentiated in culture and have the unique capacity to continue to synthesize and deposit large amounts (mg) of insoluble elastin which aggregate and from elastic fibres in vitro. 相似文献
33.
Carlos Casas-Arozamena Alexandra Cortegoso Raquel Pieiro-Perez Alicia Abalo Efigenia Arias Victoria Sampayo Ana Vilar Marta Bouso Eva Diaz Gema Moreno-Bueno Rafael Lpez-Lpez Laura Muinelo-Romay Miguel Abal Juan Cueva 《International journal of molecular sciences》2022,23(15)
Endometrial cancer (EC) is the 4th most common neoplasm of the female genital tract, with 15–20% of patients being of high risk of recurrence which leads to a significant decrease in patient survival. Current therapeutic options for patients with EC are poor, being the combined therapy of carboplatin and paclitaxel the standard of care, with limited efficacy. Therefore, new therapeutic options and better monitoring tools are needed to improve the management of the disease. In the current case report, we showcase the value of liquid biopsy analyses in a microsatellite instability EC patient with initially good prognosis that however underwent rapid progression disease within 6 months post-surgery; through the study of plasma cfDNA/ctDNA dynamics to assess the tumour evolution during treatment, as well as the study of the uterine aspirate as a valuable sample that captures the intra-tumour heterogeneity that allows a comprehensive genomic profiling of the disease to identify potential therapeutic options. Furthermore, preclinical models were generated at the time of tumour progression to assess the efficacy of the identified targeted therapies. 相似文献
34.
Pujol M.C. Massons J. Aguilo M. Diaz F. Rico M. Zaldo C. 《Quantum Electronics, IEEE Journal of》2002,38(1):93-100
The spectroscopic properties of Ho3+ laser channels in KGd(WO4)2 crystals have been investigated using optical absorption, photoluminescence, and lifetime measurements. The radiative lifetimes of Ho3+ have been calculated through a Judd-Ofelt (JO) formalism using 300-K optical absorption results. The JO parameters obtained were Ω2=15.35×10-20 cm2, Ω 4=3.79×10-20 cm2, Ω6 =1.69×10-20 cm2. The 7-300-K lifetimes obtained in diluted (8·1018 cm-3) KGW:0.1% Ho samples are: τ(5F3)≈0.9 μs, τ( 5S2)=19-3.6 μs, and τ(5F5 )≈1.1 μs. For Ho concentrations below 1.5×1020 cm-3, multiphonon emission is the main source of non radiative losses, and the temperature independent multiphonon probability in KGW is found to follow the energy gap law τph -1(0)=βexp(-αΔE), where β=1.4×10-7 s-1, and α=1.4×103 cm. Above this holmium concentration, energy transfer between Ho impurities also contributes to the losses. The spectral distributions of the Ho3+ emission cross section σEM for several laser channels are calculated in σ- and π-polarized configurations. The peak a σEM values achieved for transitions to the 5I8 level are ≈2×10-20 cm2 in the σ-polarized configuration, and three main lasing peaks at 2.02, 2.05, and 2.07 μm are envisaged inside the 5I7→5I8 channel 相似文献
35.
James E. Warner Manuel I. Diaz Wilkins Aquino Marc Bonnet 《Computational Mechanics》2014,54(3):645-659
This work focuses on the identification of heterogeneous linear elastic moduli in the context of frequency-domain, coupled acoustic-structure interaction (ASI), using either solid displacement or fluid pressure measurement data. The approach postulates the inverse problem as an optimization problem where the solution is obtained by minimizing a modified error in constitutive equation (MECE) functional. The latter measures the discrepancy in the constitutive equations that connect kinematically admissible strains and dynamically admissible stresses, while incorporating the measurement data as additional quadratic error terms. We demonstrate two strategies for selecting the MECE weighting coefficient to produce regularized solutions to the ill-posed identification problem: 1) the discrepancy principle of Morozov, and 2) an error-balance approach that selects the weight parameter as the minimizer of another functional involving the ECE and the data misfit. Numerical results demonstrate that the proposed methodology can successfully recover elastic parameters in 2D and 3D ASI systems from response measurements taken in either the solid or fluid subdomains. Furthermore, both regularization strategies are shown to produce accurate reconstructions when the measurement data is polluted with noise. The discrepancy principle is shown to produce nearly optimal solutions, while the error-balance approach, although not optimal, remains effective and does not need a priori information on the noise level. 相似文献
36.
37.
Jeffrey J. Liu Daniel E. Diaz David A. Quist Kenneth D. Karlin 《Israel journal of chemistry》2016,56(9-10):738-755
Primary copper(I)-dioxygen (O2) adducts, cupric-superoxide complexes, have been proposed intermediates in copper-containing dioxygen-activating monooxygenase and oxidase enzymes. Here, mechanisms of C−H activation by reactive copper-(di)oxygen intermediates are discussed, with an emphasis on cupric-superoxide species. Over the past 25 years, many synthetically derived cupric-superoxide model complexes have been reported. Due to the thermal instability of these intermediates, early studies focused on increasing their stability and obtaining physical characterization. More recently, in an effort to gain insight into the possible substrate oxidation step in some copper monooxygenases, several cupric-superoxide complexes have been used as surrogates to probe substrate scope and reaction mechanisms. These cupric superoxides are capable of oxidizing substrates containing weak O−H and C−H bonds. Mechanistic studies for some enzymes and model systems have supported an initial hydrogen-atom abstraction via the cupric-superoxide complex as the first step of substrate oxidation. 相似文献
38.
E. Diaz‐Bejarano F. Coletti S. Macchietto 《American Institute of Chemical Engineers》2017,63(3):984-1001
A complete, systematic approach is presented for the analysis and characterization of fouling and cleaning in refinery heat exchangers. Bringing together advanced thermo‐hydraulic dynamic models, some new formulations, and a method for dynamic analysis of plant data, it allows: extracting significant information from the data; evaluating the fouling state of the units based on thermal measurements and pressure drops, if available; identifying the range of deposit conductivity leading to realistic pressure drops, if pressure measurements are unavailable; estimating key fouling and ageing parameters; estimating the effectiveness of cleaning and surface conditions after a clean; and predicting thermal and hydraulic performance with good accuracy for other periods/exchangers operating in similar conditions. An industrial case study demonstrates the performance prediction in seamless simulations that include partial and total cleanings for over 1000 days operation. The risks of using thermal effects alone and the significant advantages of including pressure drop measurements are highlighted. © 2016 American Institute of Chemical Engineers AIChE J, 63: 984–1001, 2017 相似文献
39.
Elliptic fourier analysis of cell and nuclear shapes 总被引:1,自引:0,他引:1
40.
Yeudy F. Vargas Alzate Lluis G. Pujades Beneit Alex H. Barbat Jorge E. Hurtado Gomez Sergio A. Diaz Alvarado Diego A. Hidalgo Leiva 《Structure and Infrastructure Engineering》2018,14(6):817-829
Most of buildings and structures are usually projected according to two main axes. However, the geographical position of these buildings varies randomly. Such random distributions of the azimuthal positions of structures, in most of the cities, generally, are not accounted for when assessing their seismic risk; certainly, the direction of the seismic loads is another highly random variable. Moreover, an additional important source of uncertainty is related to the structural response, mainly due to the random character of the mechanical properties. There is a consensus that uncertainties must be considered for adequately assessing the seismic risk of structures, but these directionality effects have not been deeply explored so far. In this article, the influence of the high uncertainty involved in these input variables on the expected seismic damage is analysed. Thus, an actual earthquake, which affected the southern part of Spain, is studied. Notably, damages on a group of affected buildings, located close to the epicentre, are analysed and discussed in detail. The results show that the influence of the random azimuthal position of structures is an important source of uncertainty and that it should be taken into account when estimating the expected seismic risk in urban areas. 相似文献