Isotype-specific antibody responses to foot-and-mouth disease virus in sera and secretions of "carrier' and "non-carrier' cattle

Isotype-specific antibody responses to foot-and-mouth disease virus (FMDV) were measured in the sera and upper respiratory tract secretions of vaccinated and susceptible cattle challenged with FMDV by direct contact or by intranasal inoculation. A comparison was made between cattle that eliminated FMDV and those that developed and maintained a persistent infection. Serological and mucosal antibody responses were detected in all animals after challenge. IgA and IgM were detected before the development of IgG1 and IgG2 responses. IgM was not detected in vaccinated cattle. Challenge with FMDV elicited a prolonged biphasic secretory antibody response in FMDV "carrier' animals only. The response was detected as FMDV-specific IgA in both mucosal secretions and serum samples, which gained statistical significance (P < 0.05) by 5 weeks after challenge. This observation could represent the basis of a test to differentiate vaccinated and/or recovered convalescent cattle from FMDV "carriers'.     

Selective isolation of reversible cold sensitive variants from Chinese hamster ovary cell cultures

The fine structure and cellular associations of the large pigment cells (LPC's) of the compound eye of the house fly were studied with high voltage and conventional electron microscopy. Depending on the sector of the compound eye, the facets are either rectangular or hexagonal. The underside of each facet has indentations exactly aligned with those on top into which inserts an angulated sleeve of LPC's. Under the rectangular lens facet 6 or 8 small compact (in cross section) LPC's join four elongate LPC's. Clusters of compact cells alternate in this ring with elongate ones. Compact cells compress together and become quadrangular (in cross section) several microns below their insertion into the lens and form "building block" corners while elongate cells form "side rails" for the rectangular type of distal pseudocone enclosure. Beneath hexagonal facets all LPC's are rather elongate with out corner cells. In both facet types LPC's enclose the pseudocone for a longitudinal distance of 4 mum and then are displaced as bordering cells by a sleeve of two corneal pigment cells (CPC's), each of which encloses half of the proximal pseudocone. For the following 6 mum of longitudinal distance these concentric sleeves of CPC's and LPC's form a double layer around the pseudocone. At about 10 mum below lens base the two sleeves separate; LPC's become attenuated and extend cable-like to the basement membrane and CPC's enclose the proximal pseudocone, Semper cells and distal retinula. The junction between lens and LPC's has critical structural value in that (1) this is the sole anchorage to the lens by the lengthy remainder of the ommatidium, and (2) LPC's enclose the semiliquid pseudocone in the most distal portion of the pseudocone. In addition to vertical support, the LPC's send out numerous lateral processes that make structural contact among themselves, with the corneal pigment cells and the photoreceptor cells. The structural features of this array are discussed relative to possible physiological roles.     

Conditioned Activity and Instrumental Reinforcement Following Long-Term Oral Consumption of Cocaine by Rats.

Food-reinforced conditioned activity and instrumental responding were measured in rats after 50 weeks of continuous access to a cocaine-saccharin solution in their home cages. The elevation of conditioned activity produced by acute access to the cocaine-saccharin solution in the home cage during testing was abolished by long-term preexposure to the cocaine solution, an effect that was reversed by systemic administration of cocaine immediately prior to testing. By contrast, chronic cocaine preexposure enhanced instrumental responding for both cocaine-sucrose and pure sucrose solutions. These results support the idea that long-term cocaine exposure enhances subsequent reinforcement. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

On finite dimensional realization theory of discrete time nonlinear systems

We characterize finite dimensional realizability of discrete time nonlinear systems which have a Volterra series development with separable structure of the Volterra kernels.     

Hydrothermal catalytic production of fuels and chemicals from aquatic biomass

One of the promising avenues for biomass processing is the use of water as a reaction medium for wet or aquatic biomass. This review focuses on the hydrothermal catalytic production of fuels and chemicals from aquatic biomass. Two different regimes for conversion of aquatic biomass in hydrothermal conditions are discussed in detail. The first is hydrothermal liquefaction, and the second is hydrothermal gasification. The goals of these processes are to produce liquid‐fuel‐range hydrocarbons and methane or hydrogen, respectively. The catalytic upgrading of biocrude resulting from noncatalytic liquefaction and the stability and degradation of catalysts in high temperature water are also discussed. The review concludes with a brief discussion of the outlook for and opportunities within the field of hydrothermal catalytic valorization of biomass. Copyright © 2012 Society of Chemical Industry     

Cumulative Sum Control Charts for Monitoring Weibull‐distributed Time Between Events

The Weibull distribution can be used to effectively model many different failure mechanisms due to its inherent flexibility through the appropriate selection of a shape and a scale parameter. In this paper, we evaluate and compare the performance of three cumulative sum (CUSUM) control charts to monitor Weibull‐distributed time‐between‐event observations. The first two methods are the Weibull CUSUM chart and the exponential CUSUM (ECUSUM) chart. The latter is considered in literature to be robust to the assumption of the exponential distribution when observations have a Weibull distribution. For the third CUSUM chart included in this study, an adjustment in the design of the ECUSUM chart is used to account for the true underlying time‐between‐event distribution. This adjustment allows for the adjusted ECUSUM chart to be directly comparable to the Weibull CUSUM chart. By comparing the zero‐state average run length and average time to signal performance of the three charts, the ECUSUM chart is shown to be much less robust to departures from the exponential distribution than was previously claimed in the literature. We demonstrate the advantages of using one of the other two charts, which show surprisingly similar performance. Copyright © 2014 John Wiley & Sons, Ltd.     

A systems theoretic approach to second moment modelling of probabilistic engineering systems∗

A new approach to modelling probabilistic and stochastic engineering systems using graph theory is outlined. This approach emphasizes the introduction of probabilistic and stochastic concepts at the very beginning of the model building process. This is in marked contrast to past tendencies to add probabilistic concepts to the end of an otherwise deterministic model building process. The development of topological models founded on oriented probabilistic measurements is given. which leads to the identification of some useful statistical properties of the’ random interconnection equations’. The development of probabilistic component models is given, where a component can be characterized independently of other components. Methods for automatically building the second moment representation of the Mixed Nodal Tableau are discussed. The application of the above theory to the probabilistic analysis of a pipe network problem is briefly described.     

Numerical modelling of wind flow over a complex topography

Numerical modelling of wind flow over complex dune topography is an ambitious prospect. There is an increasing need to understand wind flow over complex topography for land planning purposes to enable prediction of sediment transport at a particular site. New surveying techniques permit the rapid development of digital terrain models, however a stumbling block is the ability of Computational Fluid Dynamics (CFD) to emulate the wind flow over such a landscape. To overcome these difficulties, it is important to establish the parameters within which such simulations can operate. This paper details an initial two-dimensional numerical model developed in order to test various modelling assumptions against experimental field wind data. Mason Bay, Stewart Island, New Zealand was chosen as an undisturbed but accessible experimental site with a prevalent on-shore wind perpendicular to a simple foredune and a complex down-wind parabolic dune system. A complex topographical two-dimensional model with vegetation represented as a roughness was compared against field data along a transect dissecting a dune system.This paper establishes that:

* Replicating the roughness patterns at the surface is important

* The inlet profile should be duplicated with care

* Modelling only a portion of the domain can have an effect on the flow patterns due to outflow effects

* There is a modelling decision to be made between the complexity of the topography and the sophistication of the turbulence model and degree to which vegetation and sand transportation are modelled.

The long-term aim is to instil confidence in numerical techniques so that such technology can be used for predictive purposes.