Effects of mite resistance mechanism of geraniums on mortality and behavior of foxglove aphid (Acyrthosiphon solani Kaltenbach)

Geraniums (Pelargonium xhortorum Bailey) possess a pest-resistance mechanism, based on glandular trichomes and the exudate they produce, that has been shown to be effective against the two-spotted spider mite (Tetranychus urticae Koch). Using an intact plant bioassay, the effectiveness of the resistance mechanism was determined for another potential pest, the foxglove aphid (Acyrthosiphon solani Kaltenbach). Comparisons were made between plant lines previously analyzed for their degree of resistance to mites, as well as their glandular trichome density and trichome exudate production. Over 100 aphid adults were bioassayed on each of the five plant lines used in the experiment. In addition to adult aphid survival, the production and survival of nymphs was determined in this bioassay. The results indicate that plant lines that are resistant to the two-spotted spider mite are also resistant to the foxglove aphid, while lines susceptible to mites are susceptible to the aphids. To evaluate the physical impediment features of the trichome exudate, the behavior of foxglove aphid nymphs was compared on two geranium lines, one a resistant line with high trichome densities and large quantities of exudate and the second a susceptible line with few trichomes and reduced exudate. A third leaf surface type was produced by washing the exudate from resistant leaves using a mildly basic buffer solution prior to the bioassay. Aphid behavior was divided into five categories: feeding or probing, resting, wandering, struggling, and immobilized. On both susceptible leaves and resistant leaves from which the exudate had been removed by washing, the aphids settled quickly and were observed with inserted stylets during most of the observation intervals. In contrast, aphids on the unwashed resistant leaf surfaces often became ensnared in the sticky trichome exudate and had difficulty in settling to probe the leaf. Physical entrapment by glandular trichome exudate appears to be an important aspect of aphid resistance in geraniums.     

Effects of dietary oils and methyl ethyl ketone peroxide onin vivo lipid peroxidation and antioxidants in rat heart and liver

Weanling male Sprague-Dawley rats were fed diets for four weeks which differed in their content of n−6 (corn oil; CO) and n−3 fatty acids (fish oil; FO), but were similar in their content of saturated and monounsaturated fatty acids and vitamin E. At the end of the four-week feeding period, each dietary group was subdivided into two groups. One group received a single placebo injection of α-tocopherol-stripped corn oil (TSCO); the other group received a single injection of the free radical generator, methyl ethyl ketone peroxide (MEKP), in TSCO. Twenty-four hours after injection, the effect of dietary oil and MEKP treatment on endogenous lipid peroxide (LPO) production (measured as methylene blue formed by the “Determiner LPO” assay), glutathione (GSH) and vitamin E content, and fatty acid composition of phosphatidylcholine and phosphatidylethanolamine in heart and liver from unfasted animals were measured. FO-fed rats had significantly heavier hearts and livers, increased levels of n−3 fatty acids in membrane phospholipids, and higher liver LPO levels than CO-fed rats. MEKP treatment resulted in significantly lower body weights and liver GSH levels. The data indicate that dietary n−3 fatty acids increase lipid peroxidation in liver somewhat more than in heart. The study also demonstrates that the effect of induced oxidative stress due to a single dose of MEKP on lipid peroxide formation and antioxidant status in tissues from unfasted animals was independent of the dietary oils.     

The crystal state binding of dithionite to deoxy-hemoglobin

The crystal state binding of sodium ditbionite to deoxyhemoglobinis reported. Dithionite has been used extensively to deoxygenatehemoglobin and myoglobin and there has been considerable interestamong users of dithionite about its effect on protein structureand binding site(s). We have determined that dithionite bindsto deoxygenated hemoglobin crystals at the interface of twomolecules in the crystal lattice. Specific residues involvedin hydrogen bonds or salt interactions with dithionite includeHisll6 and Hisll7 of the ft subunit and Lysl6 of the ß₂subunit of the adjacent hemoglobin molecule. No binding wasobserved at the symmetry related Hisll6 and 117 ß1 residues.We have shown that dithionite does not affect the native hemoglobinstructure or the binding of several allosteric inhibitors tohemoglobin and can be used to mount T state crystals in theair     

Lipid composition of the membrane released after an in vitro acrosome reaction of epididymal boar sperm

Prior to fertilization, mammalian sperm must undergo the acrosome reaction, which involves modifications of the plasma and outer acrosomal membranes followed by vesiculation and release of the membranes. The membrane fraction that was released from caudal boar sperm undergoing an in vitro acrosome-like reaction was isolated and characterized with respect to density, marker enzymes and lipid composition. This membrane had a lower phospholipid/protein ratio (mg/mg) than the sperm plasma membrane, whereas both membranes had similar molar sterol/phospholipid ratios. The major phospholipid was sphingomyelin, followed by phosphatidylethanolamine and phosphatidylcholine, whereas in the plasma membrane the order was reversed; the two major phosphoglycerides contained alkylacyl and alkenylacyl species in addition to the diacyl species. The released membrane also contained lower amounts of cholesterol sulfate and unsaturated fatty acids than the plasma membranes. These results, in combination with our studies on the changes of the sperm membranes during maturation and acrosome reaction, will allow a better understanding of the mechanism of the sperm acrosome reaction.     

Molar absorptivity of poly(methyl methacrylate) in the near-ultraviolet region

The molar absorptivities of methyl methacrylate monomer and of thermally polymerized, monomer-free poly(methyl methacrylate) have been measured in the range 2200–3000 Å, and smoothed values are presented at wavelength intervals of 20 Å over much of this range. Typical amounts of monomer present in a thermally polymerized bulk polymer and in films cast from CH₂Cl₂ solutions of the bulk polymer have been ascertained. The use of such absorptivity values to determine the amount of monomer present in polymer samples is evaluated.     

Prevention of water pollution problems in mining: The bactericide technology

In pyritic environments, the bacteriaThiobacillus ferrooxidans catalyze acid formation by increasing the oxidation rate of pyrite by a factor of one million. This acid solubilizes metals and pollutes adjacent streams and lands. Bactericide sprays during mining and waste disposal operations attack the source of the problem by preventing acid formation and metals solubilization. Used in conjunction with current water treatment systems, bactericides can dramatically reduce operating costs. Controlled release bactericides contribute to successful reclamation by providing assurance against revegetation failure and post-reclamation water quality problems that can necessitate perpetual water treatment. While inhibitingT. ferrooxidans these organic compounds aid in the establishment of beneficial heterotrophic bacteria which support vegetation. These conditions continue to persist after the bactericide is depleted from the controlled release system. Case Studies I and II show that bactericides inhibit acid generation during hard rock and coal mining operations and they are cost effective. Case Studies III and IV illustrate the improvement in water quality and vegetation after reclamation when controlled release bactericides were used. Economic analyses show cost benefits are achieved when controlled-release bactericides are part of the reclamation plan.     

Thiamine concentrations in lake whitefish eggs from the upper Great Lakes are related to maternal diet

Thiamine deficiency is responsible for reproductive impairment in several species of salmonines in the Great lakes, and is thought to be caused by the consumption of prey containing thiaminase, a thiamine-degrading enzyme. Because thiaminase levels are extremely high in dreissenid mussels, fish that prey on them may be susceptible to thiamine deficiency. We determined thiamine concentrations in lake whitefish Coregonus clupeaformis eggs from the upper Laurentian Great Lakes to assess the potential for thiamine deficiency and to determine if thiamine concentrations in lake whitefish eggs were related to maternal diet. Mean thiamine concentrations in lake whitefish eggs were highest in Lake Huron, intermediate in Lake Superior, and lowest in Lake Michigan. Some fish had thiamine concentrations below putative thresholds for lethal and sublethal effects in salmonines, suggesting that some larval lake whitefish may currently be at risk of at least sublethal effects of low thiamine concentrations, although thiamine thresholds are unknown for lake whitefish. Egg thiamine concentrations in lake whitefish eggs were statistically significantly related to isotopic carbon signatures, suggesting that egg thiamine levels were related to maternal diet, but low egg thiamine concentrations did not appear to be associated with a diet of dreissenids. Egg thiamine concentrations were not statistically significantly related to multifunction oxidase induction, suggesting that lower egg thiamine concentrations in lake whitefish were not related to contaminant exposure.     

Relationship between the attenuation of downwelling irradiance at 490 nm with the attenuation of PAR (400 nm-700 nm) in the Baltic Sea

The vertical attenuation coefficient of diffuse downwelling irradiance at 490 nm (K_d 490) is a parameter that we routinely derive from SeaWiFS images of the Baltic Sea. Here, through model simulations, we examine the relationship between K_d(490), and the vertical attenuation coefficient of PAR (K_d PAR), as this later coefficient determines the light available for aquatic photosynthesis. A simple semi-analytical model is used to predict K_d(490) and K_d(PAR), as a function of the concentrations of chlorophyll, colored dissolved organic material (CDOM), suspended inorganic, and suspended organic particulate material. A series of model simulations based on variations in these optically significant constituents over a range realistic for the Baltic Sea, are used to define the relationship between the two attenuation coefficients.

Kd(PAR)=0.6677Kd^0.6763(490).     

Priority-based rate control for service differentiation and congestion control in wireless multimedia sensor networks

In wireless multimedia sensor networks (WMSNs) a sensor node may have different types of sensor which gather different kinds of data. To support quality of service (QoS) requirements for multimedia applications having a reliable and fair transport protocol is necessary. One of the main objectives of the transport layer in WMSNs is congestion control. We observe that the information provided may have different levels of importance and argue that sensor networks should be willing to spend more resources in disseminating packets carrying more important information. Some applications of WMSNs may need to send real time traffic toward the sink node. This real time traffic requires low latency and high reliability so that immediate remedial and defensive actions can be taken when needed. Therefore, similar to wired networks, service differentiation in wireless sensor networks is also an important issue. We present a priority-based rate control mechanism for congestion control and service differentiation in WMSNs. We distinguish high priority real time traffic from low priority non-real time traffic, and service the input traffic based on its priority. Simulation results confirm the superior performance of the proposed model with respect to delays, delay variation and loss probability.