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101.
钚是环境放射性污染调查和核事故应急监测重点关注的污染核素,土壤样品中钚的定量分析包括样品消解、化学分离和仪器测量等3个步骤。为缩短样品消解时间、提高化学分离效率,对土壤样品微波消解和钚的阴离子交换分离进行了研究,建立了微波消解-阴离子交换分离-电感耦合等离子体质谱(ICP-MS)测量 同位素稀释法定量分析污染土壤样品中239Pu的流程。以6 mol/L盐酸为浸取试剂,在浸取液体积与土壤质量之比为4∶1~5∶1 mL/g时,5 g土壤样品中239Pu的加标回收率大于99%。以氢氟酸、硝酸和盐酸的混酸为消解试剂,在氢氟酸浓度为13.2 mol/L时,1 g土壤样品中239Pu的加标回收率大于94%。用所建流程测得的IAEA-375土壤标准样品中239Pu的含量与参考值无显著差异,该流程对1 g土壤样品中239Pu的检出限为84 mBq/kg。 相似文献
102.
Xiliang Du Guowen Liu Juan J. Loor Zhiyuan Fang Ryan Bucktrout Yuchen Yang Qianqian Ye Zhen Shi Taiyu Shen Xinghui Wang Zhicheng Peng Chenxu Zhao Bin Lv Dongmei Xing Yiwei Zhu Xiaobing Li Xinwei Li 《Journal of dairy science》2018,101(12):11175-11185
The ability of liver to respond to changes in nutrient availability is essential for the maintenance of metabolic homeostasis. Autophagy encompasses mechanisms of cell survival, including capturing, degrading, and recycling of intracellular proteins and organelles in lysosomes. During negative nutrient status, autophagy provides substrates to sustain cellular metabolism and hence, tissue function. Severe negative energy balance in dairy cows is associated with fatty liver. The aim of this study was to investigate the hepatic autophagy status in dairy cows with severe fatty liver and to determine associations with biomarkers of liver function and inflammation. Liver and blood samples were collected from multiparous cows diagnosed as clinically healthy (n = 15) or with severe fatty liver (n = 15) at 3 to 9 d in milk. Liver tissue was biopsied by needle puncture, and serum samples were collected on 3 consecutive days via jugular venipuncture. Concentrations of free fatty acids and β-hydroxybutyrate were greater in cows with severe fatty liver. Milk production, dry matter intake, and concentration of glucose were all lower in cows with severe fatty liver. Activities of serum aspartate aminotransferase, alanine aminotransferase, glutamate dehydrogenase, and γ-glutamyl transferase were all greater in cows with severe fatty liver. Serum concentrations of haptoglobin and serum amyloid A were also markedly greater in cows with severe fatty liver. The mRNA expression of autophagosome formation-related gene ULK1 was lower in the liver of dairy cows with severe fatty liver. However, the expression of other autophagosome formation-related genes, beclin 1 (BECN1), phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3), autophagy-related gene (ATG) 3, ATG5, and ATG12, did not differ. More important, ubiquitinated proteins, protein expression of sequestosome-1 (SQSTM1, also called p62), and microtubule-associated protein 1 light chain 3 (MAP1LC3, also called LC3)-II was greater in cows with severe fatty liver. Transmission electron microscopy revealed an increased number of autophagosomes in the liver of dairy cows with severe fatty liver. Taken together, these results indicate that excessive lipid infiltration of the liver impairs autophagic activity that may lead to cellular damage and inflammation. 相似文献
103.
κ-卡拉胶影响大豆分离蛋白乳浊液稳定性的研究 总被引:1,自引:0,他引:1
研究了卡拉胶对大豆分离蛋白乳浊液粒度分布、乳析率和离心沉淀率的影响,在此基础上分析了静置过程中乳浊液粒径与乳析率、离心沉淀率之间相关性,结果表明:乳浊液粒径随卡拉胶浓度由小到大依次为0.03%<空白样<0.06%<0.09%;而体系的表观粘度随卡拉胶浓度的增大而升高;静置过程中乳浊液顶部粒径d3,2与乳析率有较好的相关性;乳浊液底部d3,2与离心沉淀率有很好的相关性。进一步分析了其可能的作用机理:卡拉胶低浓度时,卡拉胶分子被吸附到液滴的蛋白质正电荷区域,增加了液滴间的静电排斥,从而增加了体系的絮凝稳定性;随着卡拉胶浓度增大,卡拉胶会引起体系排斥絮凝。 相似文献
104.
To solve Multimodal optimization prob-lems (MOPs), a Novel Quantum entanglement-inspired meta-heuristic framework (NMF-QE) is proposed. Its main inspirations are two concepts of quantum physics:quantum entanglement and quantum superposition. When given Proto-born particles (PBPs) of a population, these two concepts are mathematically developed to generate twin-born and combination-born particles, respectively. And if any elite-born particles would be created by a local re-searching strategy. These three or four groups of particles come together as a whole search population of NMF-QE to realize exploration and exploitation of algo-rithms. To guarantee dynamical optimization capability of NMF-QE, the individual evolutionary mechanism of some existing meta-heuristics will be adopted to iteratively create PBPs. A selected meta-heuristic is coupled with NMF-QE to present its improved variant. Numerical results show that the proposed NMF-QE can effectively improve optimization performance of meta-heuristics on MOPs. 相似文献
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107.
为研究AS1411对宫颈癌HeLa细胞的辐射增敏机理,采用γ-H2AX免疫荧光法观察HeLa细胞DNA损伤情况;流式细胞术检测AS1411对HeLa细胞周期及凋亡的影响。结果显示:经AS1411预处理的HeLa细胞核内DNA断裂点数目明显增多;AS1411预处理不影响由辐射引起的细胞周期阻滞,但能够促进辐射诱导的HeLa细胞凋亡率的增长。AS1411通过阻止由辐射引起的DNA损伤的修复及诱发细胞凋亡增加HeLa细胞的辐射敏感性,但此作用机理与细胞周期无关。 相似文献
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109.
氮离子注入选育高效发酵木糖生产L(+)-乳酸的米根霉 总被引:2,自引:0,他引:2
为了获得能够高效发酵木糖生产L( )-乳酸的菌株,采用氮离子注入诱变方法,对出发菌株米根霉RLC41-6进行改良,获得高产L( )-乳酸菌株RQ4012,发酵周期为72h,产酸能力达74.37g/L,产酸速率达1.03g/(L.h),比RLC41-6提高了1.6倍.经过多次传代实验证明该菌株具有较好的遗传稳定性. 相似文献
110.