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41.
The present paper describes the evolution of our work concerning the problem of speech recognition. Beginning with a classical hidden Markov model (HMM), we have investigated two ways to improve the performance of this basic structure. The first way was to realize a neuro-statistical hybrid by integrating a multilayer perceptron (MLP) as a posteriori probability estimator. The system was further refined by adding supplementary discriminative training (DT) based on the minimum classification error (MCE). Tests performed on a 15,000 isolated spoken-word database, showed an increase in the recognition rate from 92.2% for the HMM-based recognition system, to 94.7% for the HMM-MLP system, and then to 98.1% for the refined HMM-MLP-DT system. The second way to improve the classical HMM was to build a fuzzy-statistical hybrid, FHMM, based on a fuzzy similarity measure instead of the probabilistic measure specific to the usual statistical model. The benefits of the fuzzy measure introduction were evaluated on a vowel recognition task, and a decrease of approximately 3% in the error rate is reported.  相似文献   
42.
We develop an approach based on the subsolution–supersolution method for an elliptic system of hemivariational inequalities. The system exhibits full dependence on the gradient of the solution and is doubly coupled on both the source and multivalued terms. We prove the existence of solutions in a prescribed trapping region and, as an application, provide a criterion for obtaining positive solutions.  相似文献   
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44.
    
Favorable mechanical and electrical properties motivate the use of 2D semiconductors in flexible electronic devices. One of the main challenges here is the absence of a practical doping strategy which should provide air‐stable, tunable doping levels in a process with a low thermal budget. Here, it is shown that SU8, an epoxy‐based photoresist, can be used for nondegenerate n‐type doping of monolayer MoS2. The doping level can be finely tuned via low‐temperature annealing. The doping method exhibits good ambient stability. The high degree of mechanical flexibility and low processing temperature also allows the integration of SU8 coating with flexible MoS2 FETs, where it can provide both controllable doping and act as an encapsulation layer. The demonstrated stability of the devices to bending and exposure to water confirms the attractiveness of using SU8 in flexible electronic devices based on 2D semiconductors in a simple, versatile and scalable approach.  相似文献   
45.
    
Microbial electrosynthetic cells containing Methylobacterium extorquens were studied for the reduction of CO2 to formate by direct electron injection and redox mediator-assisted approaches, with CO2 as the sole carbon source. The formation of a biofilm on a carbon felt (CF) electrode was achieved while applying a constant potential of −0.75 V versus Ag/AgCl under CO2-saturated conditions. During the biofilm growth period, continuous H2 evolution was observed. The long-term performance for CO2 reduction of the biofilm with and without neutral red as a redox mediator was studied by an applied potential of −0.75 V versus Ag/AgCl. The neutral red was introduced into the systems in two different ways: homogeneous (dissolved in solution) and heterogeneous (electropolymerized onto the working electrode). The heterogeneous approach was investigated in the microbial system, for the first time, where the CF working electrode was coated with poly(neutral red) by the oxidative electropolymerization thereof. The formation of poly(neutral red) was characterized by spectroscopic techniques. During long-term electrolysis up to 17 weeks, the formation of formate was observed continuously with an average Faradaic efficiency of 4 %. With the contribution of neutral red, higher formate accumulation was observed. Moreover, the microbial electrosynthetic cell was characterized by means of electrochemical impedance spectroscopy to obtain more information on the CO2 reduction mechanism.  相似文献   
46.
    
Delving deeper is possible in whole-body in vivo imaging using a super-bright membrane-targeting BODIPY dye ( BD ). The dye was used to monitor homing of ex vivo fluorescently labelled neutrophils to an injured liver of dark-pigmented C57BL/6 mice. In vivo imaging system (IVIS) data conclusively showed an enhanced signal intensity and a higher signal-to-noise ratio in mice receiving neutrophils labelled with the BD dye relative to those labelled with a gold standard dye at 2 h post in vivo administration of fluorescently labelled cells. Fluorescence-activated cell sorting (FACS) confirmed that BD is nontoxic, and an exceptional cell labelling dye that opens up precision deep-organ in vivo imaging of inflammation in mice routinely used for biomedical research. The origin of enhanced performance is identified with the molecular structure and the distinct localisation of the dye within cells that enable remarkable changes in its optical parameters.  相似文献   
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This study aimed to investigate the role of Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase 2 (PLOD2) in glioblastoma (GBM) pathophysiology. To this end, PLOD2 protein expression was assessed by immunohistochemistry in two independent cohorts of patients with primary GBM (n1 = 204 and n2 = 203, respectively). Association with the outcome was tested by Kaplan–Meier, log-rank and multivariate Cox regression analysis in patients with confirmed IDH wild-type status. The biological effects and downstream mechanisms of PLOD2 were assessed in stable PLOD2 knock-down GBM cell lines. High levels of PLOD2 significantly associated with (p1 = 0.020; p2 < 0.001; log-rank) and predicted (cohort 1: HR = 1.401, CI [95%] = 1.009–1.946, p1 = 0.044; cohort 2: HR = 1.493; CI [95%] = 1.042–2.140, p2 = 0.029; Cox regression) the poor overall survival of GBM patients. PLOD2 knock-down inhibited tumor proliferation, invasion and anchorage-independent growth. MT1-MMP, CD44, CD99, Catenin D1 and MMP2 were downstream of PLOD2 in GBM cells. GBM cells produced soluble factors via PLOD2, which subsequently induced neutrophils to acquire a pro-tumor phenotype characterized by prolonged survival and the release of MMP9. Importantly, GBM patients with synchronous high levels of PLOD2 and neutrophil infiltration had significantly worse overall survival (p < 0.001; log-rank) compared to the other groups of GBM patients. These findings indicate that PLOD2 promotes GBM progression and might be a useful therapeutic target in this type of cancer.  相似文献   
48.
    
This review article explores the telocytes’ roles in inflammatory bowel diseases (IBD), presenting the mechanisms and hypotheses related to epithelial regeneration, progressive fibrosis, and dysmotility as a consequence of TCs’ reduced or absent number. Based on the presented mechanisms and hypotheses, we aim to provide a functional model to illustrate TCs’ possible roles in the normal and pathological functioning of the digestive tract. TCs are influenced by the compression of nearby blood vessels and the degree of fibrosis of the surrounding tissues and mediate these processes in response. The changes in intestinal tube vascularization induced by the movement of the food bowl, and the consequent pH changes that show an anisotropy in the thickness of the intestinal tube wall, have led to the identification of a pattern of intestinal tube development based on telocytes’ ability to communicate and modulate surrounding cell functions. In the construction of the theoretical model, given the predictable occurrence of colic in the infant, the two-layer arrangement of the nerve plexuses associated with the intestinal tube was considered to be incompletely adapted to the motility required with a diversified diet. There is resulting evidence of possible therapeutic targets for diseases associated with changes in local nerve tissue development.  相似文献   
49.
Dumitru Pavel  Jolanta Lagowski 《Polymer》2006,47(25):8389-8399
The main objective of this research is to develop and apply state-of-the-art computational tools to achieve an understanding of intermolecular interactions in molecular imprinting of chemical warfare (CW) agents into complex monomeric systems. Molecular dynamic (MD) simulations were carried out for different monomeric molecular systems in order to predict the interaction energies, the closest approach distances and the active site groups between the simulated molecular systems and different CW agents. The minimized structures of CW agents have been obtained with the use of molecular mechanics approach. NVT MD simulations at room temperature were carried out to obtain equilibrated conformations in all cases. The simulated molecular systems consisted of a ligand (CW agents) and commonly used functional monomers.During this study, it was found that electrostatic interactions play the most significant role in the formation of molecular imprinting materials. The simulated systems indicate that the functional groups of monomers interacting with ligands tend to be either -COOH or CH2CH-.  相似文献   
50.
    
The novel coronavirus disease, coined as COVID-19, is a murderous and infectious disease initiated from Wuhan, China. This killer disease has taken a large number of lives around the world and its dynamics could not be controlled so far. In this article, the spatio-temporal compartmental epidemic model of the novel disease with advection and diffusion process is projected and analyzed. To counteract these types of diseases or restrict their spread, mankind depends upon mathematical modeling and medicine to reduce, alleviate, and anticipate the behavior of disease dynamics. The existence and uniqueness of the solution for the proposed system are investigated. Also, the solution to the considered system is made possible in a well-known functions space. For this purpose, a Banach space of function is chosen and the solutions are optimized in the closed and convex subset of the space. The essential explicit estimates for the solutions are investigated for the associated auxiliary data. The numerical solution and its analysis are the crux of this study. Moreover, the consistency, stability, and positivity are the indispensable and core properties of the compartmental models that a numerical design must possess. To this end, a nonstandard finite difference numerical scheme is developed to find the numerical solutions which preserve the structural properties of the continuous system. The M-matrix theory is applied to prove the positivity of the design. The results for the consistency and stability of the design are also presented in this study. The plausibility of the projected scheme is indicated by an appropriate example. Computer simulations are also exhibited to conclude the results.  相似文献   
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