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91.
CD Louw 《Canadian Metallurgical Quarterly》1976,58(3):169-176
Renal functional abnormalities constituting the syndrome of postobstructive diuresis imply both altered tubular and glomerular membrane properties. To determine the morphologic and ultrastructural correlates of this disorder a rat model was developed and 32 postobstructed kidneys were studied by light and electron microscopy at the midpoint of diuresis and compared to 22 controls. The abnormal morphology was: dilated distal tubules and collecting ducts, isolated proximal and distal tubule cells that allowed free access of luminal contents to the basement membrane, widened terminal bars and intercellular spaces, thickening of the glomerular basement membrane and, depending upon the portion of nephron, normal or reduced adenosine triphosphatase and acid phosphatase content. In order to confirm the functional nature of the nephrons studied as well as to assess glomerular and tubular permeability, horseradish peroxidase and cytochrome c were infused. These tracers, normally permeable to the glomerular basement membrane, were found in the intercellular spaces and to a lesser extent within cell organelles in the postobstructed diuretic animals whereas controls demonstrated a retarded filtration of horseradish peroxidase, no tracer in the intercellular spaces and large amounts of tracer contained within cell organelles. Absence of enzyme activity in the medulla and reduced dark to light cell ratios in the cortical collecting ducts correlated with prior observations made by others of diminished concentration and acidification processes, respectively. An increase in adenosine triphosphatase activity and renin granules within the juxtaglomerular cells indicated increased renin activity. These observations suggest that the renal functional abnormalities of postobstructive diuresis are attributable to altered glomerular and tubular permeabilities as well as with changes in metabolic activity. 相似文献
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93.
Thirteen autistic children were compared to 13 normal children matched to them in mental age, on performance of a visual discrimination task. Form, color, and size were relevant and redundant cues. The groups did not differ significantly in mean trials to reach criterion or in breadth of learning, and both groups increased their breadth of learning after 50 trials of overtraining. Form was preferred to color and size by both autistic and normal children. Within each group, rank on mental age was highly correlated with rank in breadth of learning. Verbal and nonverbal autistic children did not differ in breadth of learning or in dimensional preference. Even nonverbal autistics equaled the performance of their normal controls. Our results suggest that overselective attention is better understood as part of a general developmental lag in cognition in autistic children than as a specific deficit underlying psychotic behavior. 相似文献
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CD Spies J Herpell O Beck C Müller F Pragst S Borg A Helander 《Canadian Metallurgical Quarterly》1999,17(1):19-27
Interactions among growth factors are important in a variety of physiological and pathological processes. The regulation of IGF-I mRNA expression by bFGF was investigated in cultured rat Müller cells and the mechanism of regulation studied. Müller cells from 1- to 3-day-old Sprague-Dawley rats were isolated and cultured with Eagle MEM+10% FCS. Cultured cells were identified by immunocytochemistry using antibodies against vimentin, carbonic anhydrase C, and glutamine synthetase. Cells of passage 1-4 were treated with bFGF, the PKC inhibitor H-7, calphostin C, the PKC activator PMA or the PKA inhibitor H-89, as well as the adenylate cyclase activator forskolin, or adenylate cyclase inhibitor SQ22536. IGF-I and bFGF expression levels were assessed by Northern blot analysis. The addition of bFGF to culture medium down-regulated IGF-I expression in a dose- and time-dependent manner. Decrease of IGF-I expression started at a bFGF concentration of 1 ng ml-1. IGF-I mRNA level declined to 44% of baseline level at 10 ng ml-1 of bFGF, and reached a trough of 40% at 50 ng ml-1. At 10 ng ml-1 of bFGF, down-regulation of IGF-I expression was observed as early as 4 hr (60%) after treatment, and reached a trough of 42% by 8 hr. The temporal and concentration dependence of IGF-I expression by addition of the PKC activator PMA, to culture medium was similar to that due to the addition of bFGF. The down-regulation of IGF-I expression by bFGF (10 ng ml-1) and PMA (0.1 microM) was blocked by the PKC inhibitors H-7 (30 microM) and calphostin C (1 microM). Forskolin (5 microM), an adenylate cyclase activator, had activator, had no effect on IGF-I expression. SQ22536 (100 microM), an adenylate cyclase inhibitor, and H-89, a PKA inhibitor, had no inhibitory effect on bFGF-induced down-regulation of IGF-I expression. These results indicate that bFGF down-regulates IGF-I expression in cultured rat M uller cells through PKC activation. 相似文献
98.
The paper (a) assesses the social and economic demands for water-related recreational, leisure and amenity facilities, (b) reviews the ways in which these demands are currently satisfied for lakes, reservoirs, rivers and canals, and (c) identifies possible future trends. The significant degree of interaction with other uses of water is also discussed. 相似文献
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J. Hensman R. Mills S.G. Pierce K. Worden M. Eaton 《Mechanical Systems and Signal Processing》2010,24(1):211-223
A standard technique in the field of non-destructive evaluation is to use acoustic emissions to characterise and locate the damage events that generate them. The location problem is typically posed in terms of the times of flight of the waves and results in an optimisation problem, which can at times be ill-posed. A method is proposed here for learning the relationship between time of flight differences and damage location using data generated by artificially stimulated acoustic emission (AE)—a classic problem of regression. A structure designed to represent a complicated aerospace component was interrogated using a laser to thermoelastically generate AE at multiple points across the structure's surface. Piezoelectric transducers were mounted on the surface of the structure, and the resulting waveforms were recorded. A Gaussian process (GP) with RBF kernels was chosen for regression. Since during AE monitoring not all events can be guaranteed to be detected by all sensors, a GP was trained on data for all possible combinations (subsets) of sensors. The inputs to the GPs were the differences in time of flight between sensors in the set, and the targets were the locations of the source of ultrasonic stimulation. Subsequent (test) data points were located by every possible GP, given the active sensors. It is shown that maps learned on a given structure can generalise effectively to nominally identical structures. 相似文献