Electrospinning of poly(vinylpyrrodine) template for formation of ZrO2 nanoclusters for enhancing properties of composite proton conducting membranes

Nanosized ZrO₂ clusters were prepared by electrospinning a poly(vinylpyrrodine) (PVP)/ZrO₂ mixture for calcination to remove PVP template and sizing. The morphological, chemical, structural, and thermal resistance changes during preparation stages were investigated using scanning electron microscope, energy-dispersive X-ray spectroscopy, transmission electron microscope, X-ray diffraction, and thermogravimetric analysis. The obtained ZrO₂ clusters were used for preparation of nanocomposite membranes by dispersion in 2,6-pyridine polybenzimidazole (2,6-Py-PBI) matrix at 5?wt% content followed by phosphoric acid (PA) doping. The ZrO₂ nanoclusters were found to be uniformly distributed in 2,6-Py-PBI/PA matrix leading to a remarkable increase in the PA doping level and proton conductivity of the obtained composite membrane.     

弯剪作用下螺栓角部连接的性能分析

研究了腹板角钢尺寸对双腹板顶底角钢的弯曲-转动性能影响。研究中采用了多个三维有限元模型,以其几何和材料特性作为影响参数。在这些模型中,所有的连接组件,如梁、柱、角钢及螺栓都采用实体单元建模。构件间的相互作用效应,如螺栓滑移和摩擦,采用表面接触算法建模。为更精确地评估连接件的性能,在栓杆上施加预拉力作为第一荷载。     

Identification and Affinity Determination of Protein-Antibody and Protein-Aptamer Epitopes by Biosensor-Mass Spectrometry Combination

Analytical methods for molecular characterization of diagnostic or therapeutic targets have recently gained high interest. This review summarizes the combination of mass spectrometry and surface plasmon resonance (SPR) biosensor analysis for identification and affinity determination of protein interactions with antibodies and DNA-aptamers. The binding constant (K_D) of a protein–antibody complex is first determined by immobilizing an antibody or DNA-aptamer on an SPR chip. A proteolytic peptide mixture is then applied to the chip, and following removal of unbound material by washing, the epitope(s) peptide(s) are eluted and identified by MALDI-MS. The SPR-MS combination was applied to a wide range of affinity pairs. Distinct epitope peptides were identified for the cardiac biomarker myoglobin (MG) both from monoclonal and polyclonal antibodies, and binding constants determined for equine and human MG provided molecular assessment of cross immunoreactivities. Mass spectrometric epitope identifications were obtained for linear, as well as for assembled (“conformational”) antibody epitopes, e.g., for the polypeptide chemokine Interleukin-8. Immobilization using protein G substantially improved surface fixation and antibody stabilities for epitope identification and affinity determination. Moreover, epitopes were successfully determined for polyclonal antibodies from biological material, such as from patient antisera upon enzyme replacement therapy of lysosomal diseases. The SPR-MS combination was also successfully applied to identify linear and assembled epitopes for DNA–aptamer interaction complexes of the tumor diagnostic protein C-Met. In summary, the SPR-MS combination has been established as a powerful molecular tool for identification of protein interaction epitopes.     

Effect of deep cryogenic processing on tensile toughness of 45WCrV7 steel

Toughness is an important property for being used in steels in engineering applications. In this research, tensile toughness of 45WCrV7 steel was measured and calculated in 10 different processing conditions. The results of tensile test showed that two samples had maximum tensile toughness. Microstructural studies demonstrated that the required condition for high tensile toughness was simultaneous increase in two microstructural factors named content and population density of the secondary carbides because a matrix which was poor of carbon and alloying elements was softened and thus increased total tensile toughness.     

Understanding the Time and Frequency Varying Characteristics of a Multipath Wireless Channel

Designing a spectrally efficient wireless channel requires a comprehensive understanding of its time and frequency varying characteristics, making it a stochastic medium of communication. These characteristics become more challenging to cater at the receiving terminal in a multipath transmission. This is because of the fading effect and Doppler shift of the transmitted frequency, specifically in cellular mobile radio systems and vehicle to vehicle communications. This paper presents the modeling, simulation, and then characterization of a cellular mobile radio multipath channel over its time and frequency varying fading gain. For this purpose, a discrete-time Finite Impulse Response (FIR) type filter of such a channel is designed, modeled, and simulated using time and frequency varying characteristics of the received signal. The simulated channel response is further analyzed in terms of coherence bandwidth, coherence time, delay spread, Doppler spread, and symbol time.

     

Single Molecule with Dual Function on Nanogold: Biofunctionalized Construct for In Vivo Photoacoustic Imaging and SERS Biosensing

Multimodal imaging provides complimentary information that is advantageous in studying both cellular and molecular mechanisms in vivo, which has tremendous potential in pre‐clinical research and clinical translational imaging. It is desirable to design probes for multimodal imaging that can be administered minimally but provides multifaceted information. Herein, we demonstrate the complementary dual functional ability of a nanoconstruct for molecular imaging in both photoacoustic (PA) and surface‐enhanced Raman scattering (SERS) biosensing simultaneously in tandem. To realize this, a group of NIR active organic molecules are designed and synthesized that possess both SERS and PA activity. Nanoconstructs realized by anchoring such molecules onto gold nanoparticles are demonstrated for targeting cancer biomarkers in vivo while providing complimentary information about biodistribution and targeting efficiency. In future, such nanoconstructs could play a major role in identifying surgical margins and also for disease monitoring in translational medicine.     

Internal electrical fault detection techniques in DFIG-based wind turbines: a review

The keys factor in making wind power one of the main power sources to meet the world’s growing energy demands is the reliability improvement of wind turbines (WTs). However, the eventuality of fault occurrence on WT com ponents cannot be avoided, especially for doubly-fed induction generator (DFIG) based WTs, which are operating in severe environments. The maintenance need increases due to unexpected faults, which in turn leads to higher operating cost and poor reliability. Extensive investigation into DFIG internal fault detection techniques has been carried out in the last decade. This paper presents a detailed review of these techniques. It discusses the methods that can be used to detect internal electrical faults in a DFIG stator, rotor, or both. A novel sorting technique is presented which takes into consideration different parameters such as fault location, detection technique, and DFIG modelling. The main mathematical representation used to detect these faults is presented to allow an easier and faster under standing of each method. In addition, a comparison is carried out in every section to illustrate the main differences, advantages, and disadvantages of every method and/or model. Some real monitoring systems available in the market are presented. Finally, recommendations for the challenges, future work, and main gaps in the field of internal faults in a DFIG are presented. This review is organized in a tutorial manner, to be an effective guide for future research for enhancing the reliability of DFIG-based WTs.     

Engineering geological properties of the pyroclastic cone-shaped rocky houses of Kandovan,Iran

Bulletin of Engineering Geology and the Environment - Kandovan is one of the geo-tourism attractions in the East Azarbaijan Province of Iran, where rural houses were carved within the cone-shaped...