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91.
Kilian Simmet Eckhard Wolf Valeri Zakhartchenko 《International journal of molecular sciences》2021,22(1)
The nucleus of a differentiated cell can be reprogrammed to a totipotent state by exposure to the cytoplasm of an enucleated oocyte, and the reconstructed nuclear transfer embryo can give rise to an entire organism. Somatic cell nuclear transfer (SCNT) has important implications in animal biotechnology and provides a unique model for studying epigenetic barriers to successful nuclear reprogramming and for testing novel concepts to overcome them. While initial strategies aimed at modulating the global DNA methylation level and states of various histone protein modifications, recent studies use evidence-based approaches to influence specific epigenetic mechanisms in a targeted manner. In this review, we describe—based on the growing number of reports published during recent decades—in detail where, when, and how manipulations of the epigenome of donor cells and reconstructed SCNT embryos can be performed to optimize the process of molecular reprogramming and the outcome of nuclear transfer cloning. 相似文献
92.
This study analyzed the methodology and applicability of multivariate cumulative sum (MCUSUM) charts for early mastitis and lameness detection. Data used were recorded on the Karkendamm dairy research farm, Germany, between August 2008 and December 2010. Data of 328 and 315 cows in their first 200 d in milk were analyzed for mastitis and lameness detection, respectively. Mastitis as well as lameness was specified according to veterinary treatments. Both diseases were defined as disease blocks. Different disease definitions for mastitis and lameness (2 for mastitis and 3 for lameness) varied solely in the sequence length of the blocks. Only the days before the treatment were included in the disease blocks. Milk electrical conductivity, milk yield, and feeding patterns (feed intake, number of trough visits, and feeding time) were used for the recognition of mastitis. Pedometer activity and feeding patterns were used for lameness detection. To exclude biological trends and obtain independent observations, the values of each input variable were either preprocessed by wavelet filters or a multivariate vector autoregressive model. The residuals generated between the observed and filtered or observed and forecast values, respectively, were then transferred to a classic or self-starting MCUSUM chart. The combination of the 2 preprocessing methods with each of the 2 MCUSUM sum charts resulted in 4 combined monitoring systems. For mastitis as well as lameness detection requiring a block sensitivity of at least 70%, all 4 of the combined monitoring systems used revealed similar results within each of the disease definitions. Specificities of 73 to 80% and error rates of 99.6% were achieved for mastitis. The results for lameness showed that the definitions used obtained specificities of up to 81% and error rates of 99.1%. The results indicate that the monitoring systems with these study characteristics have appealing features for mastitis and lameness detection. However, they are not yet directly applicable for practical implementations. 相似文献
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Filters used for building general ventilation are tested since more than 20 years, and there is always a heavy discussion about the usability of test data under lab conditions. New is the introduction of a minimum efficiency (EN779; draft). In this study filters are mounted in the ventilation system for 3 and 6 month. Afterwards fractional efficiencies are measured with a DEHS aerosol in a test rig. In addition tests are performed using diesel soot as loading aerosol or isopropanol to reduce the electrostatic charge on the synthetic fibers. The results show a strong influence of the efficiency on the test procedure and very loosely agreement of these results with those using ASHRAE dust. 相似文献
95.
Scanning electrochemical microscopy (SECM) in its alternating current mode (AC-SECM) has been of growing importance to detect and interpret local variations of electrochemical surface properties with high spatial resolution. The sensitivity of AC-SECM and hence the quality of imaging was found to be highly dependent on the experimental conditions. It was commonly judged using approach curves in the distance domain. As an alternative to current-distance approach curves, the coordinate system based on real and imaginary part of impedance is suggested for their display. In this framework, the difference between the impedance vector magnitude, real or imaginary part of impedance over neighboring surfaces is considered as a measure of imaging contrast. The variable which grants maximum difference is selected for imaging while measurement conditions associated with this maximum indicate optimum scanning conditions. The methodology of determining these optimized parameters is laid out in this contribution and examples are given. Since the optimum parameters are identified before the actual lateral imaging, this time-saving method facilitates the visualization of local electrochemical properties at the highest possible quality. 相似文献
96.
In 1995 at the integral test facility ISB-VVER in Elektrogorsk near Moscow natural circulation experiments were performed, which were scientifically accompanied by the Forschungszentrum Rossendorf. These experiments were the first of this kind at a test facility, which models VVER-1000 thermalhydraulics. Using the code ATHLET which is being developed by ‘Gesellschaft für Anlagen und Reaktorsicherheit’, pre- and post-test calculations were done to determine the thermalhydraulic events to be expected and to define and tune the boundary conditions of the test. The conditions found for natural circulation instabilities and cold leg loop seal clearing could be confirmed by the tests. Besides the thermalhydraulic standard measuring system, the facility was equipped with needle shaped conductivity probes for measuring the local void fractions. 相似文献
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100.
Heide Budde-Meiwes Julia KowalDirk Uwe Sauer Eckhard Karden 《Journal of power sources》2011,196(23):10415-10423
Many battery simulation models, but also electrochemical interpretations are based on impedance spectroscopy. However, the impedance of a battery is influenced by various factors, e.g. in the case of a lead-acid battery: state of charge (SOC), charging or discharging, superimposed dc current, short-term history or homogeneity of the electrolyte. This paper analyses the impact of those factors on impedance spectra of lead-acid batteries. The results show that very detailed information about the conditions during the measurement is crucial for the correct interpretation of a spectrum. 相似文献