Proteomic and peptidomic characterisation of beer: Immunological and technological implications

In this study, the protein and peptide fractions of two commercial Italian barley malt beers, made with different processes by the same producer, have been analysed with a combined immunochemical and mass spectrometry approach. The “gluten” content of beer samples, measured with the R5 monoclonal antibody, was below the caution limit proposed by the Codex Alimentarius for gluten-free foods. The proteomic approach allowed to identify a 17 kDa avenin-like protein partially homologous to hordeins, that was particularly abundant in foam, in addition to the already reported barley albumins (Z₄-barley and ns-LTPs) and to minor amounts of yeast glycolytic enzymes. No intact hordeins were detected, although fragments derived from γ₃- and B-hordein were present. In consideration of the many implications of the protein/peptide pattern, these data provide useful information to improve quality and safety of beer.     

Synthetic peptides as substrate for assaying the proteolytic activity of Lactobacillus helveticus

Four Lactobacillus helveticus strains were studied for proteolytic capacity and general aminopeptidase (AP) and X-Pro dipeptidyl aminopeptidase (DAP) activity. The rate of hydrolysis and the activity against synthetic substrates with N-terminal residues of Arg, Lys, Leu, Glu or Pro, varied markedly among the strains. The X-Pro DAP activity was consistently high. The crude cell-wall and cytoplasm extracts from strain Lb. helveticus ISLC59 were analysed thoroughly for their proteolysis ability by using four synthetic peptide substrates, including alpha(s)1-CN(f1-23). Peptides formed during in vitro hydrolysis of the synthetic substrates by cell wall and cytoplasm preparations were identified by LC-ESI/MS. In doing so, it was possible to infer a prevalent endopeptidase activity splitting Lys7-His8 and Gln13-Glu14 bonds in the cytoplasm, and to deduce a secondary activity, which hydrolysed Glu14-Val15, Leu16-Asn17, Glu18-Asn19 and Lys3-His4 bonds lacking in the cell-wall. The presence of exopeptidases, as mainly AP, DAP, and carboxypeptidase (CPase) was deduced from the formation of several N- and C-terminally truncated peptides sets. The AP activity was higher in the cell-wall layer, where CPase activity was absent. The in vitro assays with cell extracts of the Lb. helveticus ISLC59 strain revealed extensive exopeptidase and endopeptidase activities. In several cases, the hydrolytic system of Lb. helveticus that splits in vitro alpha(s)1-CN(f1-23) peptide bonds was similar to that of Lactococcus lactis. The effects were also compared with those occurring in vivo in hard cheese such as Grana Padano.     

Compressibility and flow properties of a cohesive limestone powder in a medium pressure range

The most important design parameters for roller presses can be referred to flow characteristic of bulk materials. Usually the flow properties are measured in the low stress range 1–50 kPa at the shear rate about 1 mm/min. But this does not fit the stressing conditions in the roller press. Press shear cell was used for shear tests with cohesive limestone powder from Gummern in the so-called medium pressure range 50–1000 kPa.     

A Graph-Theoretic Framework for Assessing the Resilience of Sectorised Water Distribution Networks

Water utilities face a challenge in maintaining a good quality of service under a wide range of operational management and failure conditions. Tools for assessing the resilience of water distribution networks are therefore essential for both operational and maintenance optimization. In this paper, a novel graph-theoretic approach for the assessment of resilience for large scale water distribution networks is presented. This is of great importance for the management of large scale water distribution systems, most models containing up to hundreds of thousands of pipes and nodes. The proposed framework is mainly based on quantifying the redundancy and capacity of all possible routes from demand nodes to their supply sources. This approach works well with large network sizes since it does not rely on precise hydraulic simulations, which require complex calibration processes and computation, while remaining meaningful from a physical and a topological point of view. The proposal is also tailored for the analysis of sectorised networks through a novel multiscale method for analysing connectivity, which is successfully tested in operational utility network models made of more than 100,000 nodes and 110,000 pipes.     

The impact of metal transport processes on bioavailability of free and complex metal ions in methanogenic granular sludge

Bioavailability of metals in anaerobic granular sludge has been extensively studied, because it can have a major effect on metal limitation and metal toxicity to microorganisms present in the sludge. Bioavailability of metals can be manipulated by bonding to complexing molecules such as ethylenediaminetetraacetate (EDTA) or diethylenetriaminepentaacetate (DTPA). It has been shown that although the stimulating effect of the complexed metal species (e.g. [CoEDTA](2-)) is very fast, it is not sustainable when applied to metal-limited continuously operated reactors. The present paper describes transport phenomena taking place inside single methanogenic granules when the granules are exposed to various metal species. This was done using magnetic resonance imaging (MRI). The MRI results were subsequently related to technological observations such as changes in methanogenic activity upon cobalt injection into cobalt-limited up-flow anaerobic sludge blanket (UASB) reactors. It was shown that transport of complexed metal species is fast (minutes to tens of minutes) and complexed metal can therefore quickly reach the entire volume of the granule. Free metal species tend to interact with the granular matrix resulting in slower transport (tens of minutes to hours) but higher final metal concentrations.     

Positioning and immobilization of individual quantum dots with nanoscale precision

We demonstrate a technique for the precise immobilization of nanoscale objects at accurate positions on two-dimensional surfaces. We have developed a water-based photoresist that causes nanostructures such as colloidal quantum dots to segregate to a thin layer at surfaces. By combining this material with electroosmotic feedback control, we demonstrate the ability to position selected, individual quantum dots at specific locations and to immobilize them with 130 nm precision via localized UV exposure.     

Internet: A certain insecurity

Despite the rapidly increasing popularity of the Internet, many still view this new communication system as a fad that will pass as quickly as it has emerged. In many cases, management defers investments in Internet technology and skills until mañana and a day. Who can blame them? Three decades of experience with the information-technology industry have taught these managers that the economic lifespan of its products and companies is insanely short, and that caution is a successful strategy to avoid irresponsible investments. However, as caution degrades into neglect, management degrades into dismissing new products and services without thoroughly vetting them.