Urinary Excretion of Ecdysterone and Its Metabolites Following Spinach Consumption

Scope

The phytosteroid ecdysterone is present in spinach. In this study, the urinary elimination of ecdysterone and its metabolites in humans is investigated following spinach consumption of two different culinary preparations.

Methods and results

Eight participants (four males, four females) ingested 950 (27.1) g sautéed spinach (average [±standard deviation (SD)]) and 912 (70.6) g spinach smoothie as second intervention after washout. Post-administration urines are analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). After intake of both preparations, ecdysterone and two metabolites, 14-deoxy-ecdysterone, and 14-deoxy-poststerone, are excreted in urine. The maximum concentration of ecdysterone is ranging from 0.09 to 0.41 µg mL⁻¹ after sautéed spinach and 0.08–0.74 µg mL⁻¹ after smoothie ingestion. The total excreted amount (mean% [±SD]) in the urine as a parent drug plus the metabolites is only 1.4 (1.0) for both sautéed spinach and smoothie. The apparent sex related differences in 14-deoxy-poststerone excretion will need further investigations.

Conclusion

Only a small proportion of ecdysterone from spinach is excreted into urine. No significant differences are found in concentration and recovered amount (%) of ecdysterone, 14-deoxy-ecdysterone, and 14-deoxy-poststerone in urine between sautéed spinach and smoothie ingestion. A discrimination between ecdysterone from food or preparations will be challenging based on urinary concentrations only, at least for later post-administration samples.     

Electron energy loss near edge structure on the nitrogen K-edge in vanadium nitrides

This paper presents electron energy-loss near-edge structure (ELNES) data for the N K edges of vanadium nitrides. By rapid thermal processing of vanadium layers in pure nitrogen at high temperatures the two known vanadium nitrides, VN and V₂N, have been prepared. The phases have been checked by electron diffraction and quantitative electron energy-loss spectroscopy (EELS) analysis. Because their crystallographical structures are different, they also exhibit different ELNES features, which can be used as fingerprints for rapidly distinguishing between VN and V₂N. The experimental findings are supported by modelling the N K edge using a band structure approach (full linearized augmented plane wave method).     

The Adaptive Solar Facade: From concept to prototypes

The Adaptive Solar Facade (ASF) is a modular, highly integrated dynamic building facade.The energetic behavior as well as the architectural expression of the facade can be controlled with high spatio-temporal resolution through individually addressable modules. We present the general design process, the current mechanical design, and simulation results on photovoltaic power production and building energy consumption. We introduce the controller concept and show results on solar tracking as well as user interaction. Lastly, we present our current and planned prototypes.     

On the generation of test patterns for multiple faults

This article presents a new method to generate test patterns for multiple stuck-at faults in combinational circuits. We assume the presence of all multiple faults of all multiplicities and we do not resort to their explicit enumeration: the target fault is a single component of possibly several multiple faults. New line and gate models are introduced to handle multiple fault effect propagation through the circuits. The method tries to generate test conditions that propagate the effect of the target fault to primary outputs. When these conditions are fulfilled, the input vector is a test for the target fault and it is guaranteed that all multiple faults of all multiplicities containing the target fault as component are also detected. The method uses similar techniques to those in FAN and SOCRATES algorithms to guide the search part of the algorithm, and includes several new heuristics to enhance the performance and fault detection capability. Experiments performed on the ISCAS'85 benchmark circuits show that test sets for multiple faults can be generated with high fault coverage and a reasonable increase in cost over test generation for single stuck-at faults.     

Helium droplet assisted synthesis of plasmonic Ag@ZnO core@shell nanoparticles

Plasmonic Ag@ZnO core@shell nanoparticles are formed by synthesis inside helium droplets with subsequent deposition and controlled oxidation. The particle size and shape can be controlled from spherical sub-10 nm particles to larger elongated structures. An advantage of the method is the complete absence of solvents, precursors, and other chemical agents. The obtained particle morphology and elemental composition have been analyzed by scanning transmission electron microscopy (STEM) and energy dispersive X-ray spectroscopy (EDS). The results reveal that the produced particles form a closed and homogeneous ZnO layer around a 2–3 nm Ag core with a uniform thickness of (1.33 ± 0.15) nm and (1.63 ± 0.31) nm for spherical and wire-like particles, respectively. The results are supported by ultraviolet photoelectron spectroscopy (UPS), which indicates a fully oxidized shell layer for the particles studied by STEM. The plasmonic properties of the produced spherical Ag@ZnO core@shell particles are investigated by two-photon photoelectron (2PPE) spectroscopy. Upon excitation of the localized surface plasmon resonance in Ag at around 3 eV, plasmonic enhancement leads to the liberation of electrons with high kinetic energy. This is observed for both Ag and Ag@ZnO particles, showing that even if a Ag cluster is covered by the ZnO layer, a plasmonic enhancement can be observed by photoelectron spectroscopy.

     

Enzymatic determination of sorbic acid

 An enzymatic method for the determination of sorbic acid based on the spectrophotometric measurement of sorbyl coenzyme A (sorbyl CoA) at 300 nm was developed. Sorbic acid is converted to sorbyl CoA with acyl CoA synthetase (EC 6.2.1.3) in the presence of coenzyme A and adenosine-5′-triphosphate. The reaction is made quantitative by irreversibly hydrolyzing pyrophosphate formed during the acylation reaction to phosphate in the presence of inorganic pyrophosphatase (EC 3.6.1.1). The absorbance measured at 300 nm is specific for sorbyl CoA and its value is proportional to the amount of sorbic acid in the sample solution. Accuracy, repeatability and recovery rates after standard addition to sample solutions were evaluated. All steps of the enzymatic analysis were carried out on an automatic centrifugal analyzer. The method is fast, precise and reliable, and therefore well suited for routine determinations, especially for high sample throughputs. Received: 7 June 1999 / Revised version: 12 August 1999     

Low-noise operation modes of a passively mode-locked fiber laser

Optimum low-noise operation modes of a passively mode-locked fiber laser are described and characterized. The laser produces 60 fs bandwidth limited pulses with root mean square amplitude fluctuations of 0.4% in a frequency-band from 30 Hz to 100 kHz. It is shown that low-noise operation up to 5 dB below the amplitude noise of the pump laser is possible with overall negative cavity group-velocity dispersion and a large difference between the intracavity loss under free-running and mode-locked laser operation. Additional frequency stabilization reduces the timing jitter to 4 fs in a frequency band from 1 kHz to 100 kHz and 110 fs in the 30 Hz to 100 kHz band     

Estimated benefits of glycemic control in microvascular complications in type 2 diabetes

BACKGROUND: The benefits of intensive glycemic control in patients with type 2 diabetes are not well quantified. It is therefore not clear which patients will benefit most from aggressive glycemic control. OBJECTIVE: To evaluate the efficacy of glycemic control in type 2 diabetes. DESIGN: Markov decision model. PATIENTS: Diabetic patients at a health maintenance organization. MAIN OUTCOME MEASURES: Risks for developing blindness and end-stage renal disease; number of patients and patient-years needed to treat to prevent complications. RESULTS: For a patient in whom diabetes developed before 50 years of age, reducing hemoglobin A1c levels from 9% to 7% results in an estimated 2.3-percentage point decrease (from 2.6% to 0.3%) in lifetime risk for blindness due to retinopathy. The same change in a patient with diabetes onset at 65 years of age would be expected to decrease the risk for blindness by 0.5 percentage points (from 0.5% to < 0.1%). However, the Markov model predicts substantially greater benefit when moving from poor to moderate glycemic control than when moving from moderate to almost-normal glycemic control. Targeting less than 20% of the patients at one health maintenance organization for intensified therapy may prevent more than 80% of the preventable patient-time spent blind. The risks for end-stage renal disease and the risk reduction provided by improved glycemic control are lower than those for blindness. CONCLUSIONS: This probability model, based on extrapolation from the experience with type 1 diabetes, suggests that patients with early onset of type 2 diabetes will accrue substantial benefit from almost-normal glycemic control. In patients with later onset, moderate glycemic control prevents most end-stage complications caused by microvascular disease. These results have important implications for informing patients and allocating health care resources.