Deconstructing the Effects of Matrix Elasticity and Geometry in Mesenchymal Stem Cell Lineage Commitment

A wide variety of environmental factors including physical and biochemical signals are responsible for stem cell behavior and function. In particular, matrix elasticity and cell shape have been shown to determine stem cell function, yet little is known about the interplay between how these physical cues control cell differentiation. For the first time, by using ultraviolet (UV) lithography to pattern poly(ethylene) glycol (PEG) hydrogels, it is possible to manufacture microenvironments capable of parsing the effects of matrix elasticity, cell shape, and cell size in order to explore the relationship between matrix elasticity and cell shape in mesenchymal stem cell (MSC) lineage commitment. These data show that cells cultured on 1000 μm² circles, squares, and rectangles are primarily adipogenic lineage regardless of matrix elasticity, while cells cultured on 2500 and 5000 μm² shapes more heavily depend on shape and elasticity for lineage specification. It is further characterized how modifying the cell cytoskeleton through pharmacological inhibitors can modify cell behavior. By showing MSC lineage commitment relationships due to physical signals, this study highlights the importance of cell shape and matrix elasticity in further understanding stem cell behavior for future tissue engineering strategies.     

Composite Living Fibers for Creating Tissue Constructs Using Textile Techniques

The fabrication of cell‐laden structures with anisotropic mechanical properties while having a precise control over the distribution of different cell types within the constructs is important for many tissue engineering applications. Automated textile technologies for making fabrics allow simultaneous control over the color pattern and directional mechanical properties. The use of textile techniques in tissue engineering, however, demands the presence of cell‐laden fibers that can withstand the mechanical stresses during the assembly process. Here, the concept of composite living fibers (CLFs) in which a core of load bearing synthetic polymer is coated by a hydrogel layer containing cells or microparticles is introduced. The core thread is drawn sequentially through reservoirs containing a cell‐laden prepolymer and a crosslinking reagent. The thickness of the hydrogel layer increases linearly with to the drawing speed and the prepolymer viscosity. CLFs are fabricated and assembled using regular textile processes including weaving, knitting, braiding, winding, and embroidering, to form cell‐laden structures. Cellular viability and metabolic activity are preserved during CLF fabrication and assembly, demonstrating the feasibility of using these processes for engineering functional 3D tissue constructs.     

Penetration–elimination method for five-axis CNC machining of sculptured surfaces

In this paper, a new tool positioning strategy for five-axis machining, called the penetration–elimination method (PEM), is introduced. The PEM gains from two innovative techniques that can considerably improve the computational efficiency during the calculation of the optimal tool orientations.The first technique includes developing a quantitative definition for the gouging concept and using this definition in conjunction with powerful numerical root-finder algorithms to determine the optimized tool orientations. The second technique dynamically detects the ineffective grid points and drops them from calculations and consequently takes a great role in reducing the computational burden. The ability of the PEM in removing various types of gouging has been shown via several examples. The computational efficiency of the PEM has been compared with another recently described method named the arc-intersect method (AIM). This comparison shows that although the two methods reach the same solution for the tool orientation, the PEM is averagely 7.5 times faster than the AIM.     

Fuzzy-based multiscale edge detection

A new fuzzy-based multiscale edge detection technique is presented. The proposed approach achieves optimal edge detection using the wavelet decomposition of the original signal followed by a novel fuzzy-based decision technique that is applied across the scales. Results indicate a significant improvement in locating edges compared to other multiscale approaches.     

Primary lymphoproliferative disorders of the breast

Primary breast lymphoproliferative disorders are rare lesions and include both the malignant lymphomas and the benign pseudolymphomas. We reviewed 4,491 consecutive cases of breast cancer diagnosed and treated between 1973 and 1988. Patients with lymphoma in other sites and those with lymphomas limited to axillary nodes were excluded. RESULTS. Five patients (0.11%) presented with primary lymphoreticular lesions, of which three were primary non-Hodgkin's lymphoma and two were pseudolymphomas. Patients were followed clinically through to the present time or until death occurred. Surgical procedures included incisional or excisional biopsy in four patients and modified radical mastectomy in one. Two patients received chemo-therapy and one received radiotherapy. One patient with pseudolymphoma subsequently developed infiltrating ductal carcinoma of the same breast. Three patients with primary breast non-Hodgkin's lymphoma died within the follow-up period, with a mean survival of 33 months. CONCLUSIONS. We conclude that primary breast lymphoma is a rare and aggressive breast malignancy with a poor prognosis despite different treatment options.     

Quantitative automated inspection of standard parts using machine vision

On line automated visual inspection for quality and process control is becoming a very important requirement in an automated manufacturing environment. This paper examines the possibility of real-time inspection of standard part using machine vision.     

Noise cross PSD estimation using phase information in diffuse noise field

Among various speech enhancement methods, two-microphone noise reduction systems are utilized for their low cost implementation and acceptable performance. Coherence-based methods are well known as efficient two-microphone noise reduction techniques. These techniques, however, do not work well when the received noise signals are correlated. Coherence-based methods can be improved when the cross power spectral density (CPSD) of input noises is available. In this paper, we propose a new method for estimating noise CPSD based on the assumption of a diffuse noise field. With this assumption, we estimate the noise CPSD using phase information. Then, the estimated noise CPSD is used to calculate a coherence-based gain filter which is then employed to enhance noisy signals. We compare the proposed phase-based noise CPSD estimation with a noise CPSD estimation technique based on a voice activity detector (VAD), both of which are herein separately employed in a two-microphone speech enhancement configuration. The comparison shows that the two-microphone speech enhancement scheme utilizing the proposed noise CPSD estimation technique outperforms the enhancement system using the VAD-based noise CPSD estimation.     

Influence of PP‐g‐MA on morphology,mechanical properties and deformation mechanism of copolypropylene/clay nanocomposite

Copolypropylene/organoclay nanocomposites are prepared by melt intercalation method in this research. Two different routes for addition of compatibilizer are examined, i.e. addition in the twin‐screw extruder along with the polymer and the clay powder simultaneously and premixing the compatibilizer with the reinforcement in a batch mixer before addition to the polypropylene (PP) matrix. Morphology, tensile and impact properties and deformation mechanisms of the samples made via two procedures are studied and compared with those of the noncompatibilized system. To study the structure of nanocomposites, x‐ray diffraction and transmission electron microscopy techniques are utilized. The deformation mechanisms of different samples are examined via reflected and transmitted optical microscopy. The results reveal that introduction of compatibilizer and also the procedure in which the compatibilizer is added to the compound, affect structure and mechanical properties of nanocomposite. The elastic modulus of PP‐clay nanocomposite has increased 11.5% with incorporation of compatibilizer. Also, introduction of organoclay without compatibilizer facilitates crazing at the notch tip of PP in 3PB testing. Incorporation of compatibilizer, however, makes difficulties in initiation and growth of crazes at the notch tip. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009     

The effects of ginger intake on weight loss and metabolic profiles among overweight and obese subjects: A systematic review and meta-analysis of randomized controlled trials

This systematic review and meta-analysis of randomized controlled trials (RCTs) was performed to summarize the effect of ginger intake on weight loss, glycemic control and lipid profiles among overweight and obese subjects. We searched the following databases through November 2017: MEDLINE, EMBASE, Web of Science, and Cochrane Central Register of Controlled Trials. The relevant data were extracted and assessed for quality of the studies according to the Cochrane risk of bias tool. Data were pooled using the inverse variance method and expressed as Standardized Mean Difference (SMD) with 95% Confidence Intervals (95% CI). Heterogeneity between studies was assessed by the Cochran Q statistic and I-squared tests (I²). Overall, 14 studies were included in the meta-analyses. Fourteen RCTs with 473 subjects were included in our meta-analysis. The results indicated that the supplementation with ginger significantly decreased body weight (BW) (SMD ?0.66; 95% CI, ?1.31, ?0.01; P = 0.04), waist-to-hip ratio (WHR) (SMD ?0.49; 95% CI, ?0.82, ?0.17; P = 0.003), hip ratio (HR) (SMD ?0.42; 95% CI, ?0.77, ?0.08; P = 0.01), fasting glucose (SMD ?0.68; 95% CI, ?1.23, ?0.05; P = 0.03) and insulin resistance index (HOMA-IR) (SMD ?1.67; 95% CI, ?2.86, ?0.48; P = 0.006), and significantly increased HDL-cholesterol levels (SMD 0.40; 95% CI, 0.10, 0.70; P = 0.009). We found no detrimental effect of ginger on body mass index (BMI) (SMD ?0.65; 95% CI, ?1.36, 0.06; P = 0.074), insulin (SMD ?0.54; 95% CI, ?1.43, 0.35; P = 0.23), triglycerides (SMD ?0.27; 95% CI, ?0.71, 0.18; P = 0.24), total- (SMD ?0.20; 95% CI, ?0.58, 0.18; P = 0.30) and LDL-cholesterol (SMD ?0.13; 95% CI, ?0.51, 0.24; P = 0.48). Overall, the current meta-analysis demonstrated that ginger intake reduced BW, WHR, HR, fasting glucose and HOMA-IR, and increased HDL-cholesterol, but did not affect insulin, BMI, triglycerides, total- and LDL-cholesterol levels.