Application of plant-based antimicrobials for the growth inhibition of clostridia in pressed beet pulp silage

BACKGROUND: In this study the inhibition of hop beta acids on the growth of clostridia in soil‐contaminated pressed sugar beet pulp silages was investigated. Hop beta acids are natural substances which display their effect at low concentrations. Fresh pressed beet pulp material was mixed with soil to artificially contaminate it with clostridia. Laboratory silos were filled with the substrate, stored at 25 °C and opened for sampling at 0, 2, 8, 15, 30, 60, and 90 days. The impact on clostridial growth during silage fermentation was monitored by determination of the pH value and dry matter content, as well as chemical analysis of the fermentation products. Throughout the experiments, the effect of a commercial silage inoculant based on lactic acid bacteria (LAB) and hop‐resistant LAB were examined with and without the combination of plant‐based antimicrobials. RESULTS: Results indicate that in contaminated silage samples without any additives high butyric acid contents occurred due to clostridial growth. This spoilage could not be suppressed by the application of LAB, whereas the combined application of LAB and hop beta acids significantly improved silage quality, which was reflected by favourable organic acid composition (P < 0.05). CONCLUSION: The experimental data indicate that the application of hop beta acids improves the preservation effect of LAB in suppressing clostridial growth in silages and thus demonstrates some potential for the combined use of plant‐based antimicrobials and LAB. Copyright © 2011 Society of Chemical Industry     

Combination of biological mechanisms for a concept study of a fracture-tolerant bio-inspired ceramic composite material

The biological materials nacre and wood are renowned for their impressive combination of toughness and strength. The key mechanisms of these highly complex structures are crack deflection at weak interfaces, crack bridging, functional gradients and reinforcing elements. These principles were applied to a more fracture-tolerant model material which combined porous stiff ceramic layers, manufactured by freeze casting, infiltrated and bonded by a polymer phase reinforced with fabric layers. In the hybrid composites, crack deflection occurred at the ceramic–fabric interface and the intact fabric layers served as crack-bridging elements. Fabric-reinforced epoxy layers stabilized the fracture behaviour and delayed catastrophic failure of the material. The influence of the different components was analysed by varying the ceramic, fabric and interface properties. More ductile fabrics lead to larger strain to failure and more crack bridging but reduced the composite strength and stiffness after initial cracking. Higher elastic mismatch between the components improved crack deflection and bridging but resulted in deterred load transfer and a lower strength. The stiffness and strength of the ceramic layers influenced the elastic properties of the laminar composite and the initial crack resistance. Flaw tolerance was increased with polymer infiltration. We show with our hybrid ceramic–fabric composite as a bio-inspired concept study how fracture toughness, work of fracture and tolerance for cracking can be tailored when the contributing factors, i.e. the ceramic, the fabric and their interface, are modified.     

Deliberative Performance of Television News in Three Types of Democracy: Insights from the United States,Germany, and Russia

We show that television news is considerably more deliberative in established (United States, Germany) than in defective democracies (Russia) and slightly more deliberative in a power‐sharing political system (Germany) than in a power‐concentrating system (United States). We further demonstrate that public‐service channels, nonpartisan programs, and in‐depth news shows make stronger overall contributions toward deliberativeness than their respective counterparts. While national news cultures produce distinct national styles of mediated deliberation, individual channels in the United States (Fox, CNN) and Russia (REN) cut across these national patterns. The significance of deliberative media content for citizens and political elites is discussed.     

Tailoring the Surface of Aluminum-Enriched Diatom Biosilica

Aluminosilicates are widely used as sorbent materials, ion exchangers, and catalysts. While they are often synthesized by hydrothermal methods, microorganisms may open “green” synthesis ways. Diatoms can incorporate aluminum into their micro- and nanostructured silica-based cell walls. Thus, diatoms create intricately structured aluminosilicate materials. The present study investigates not only possible morphological changes during the in vivo Al-enrichment of the diatom species Thalassiosira pseudonana, but also the increase of the specific surface area of Al-enriched biosilica in vitro by etching with alkaline solutions.     

A „Magic Mushroom“ Multi-Product Sesquiterpene Synthase

Psilocybe “magic mushrooms” are chemically well understood for their psychotropic tryptamines. However, the diversity of their other specialized metabolites, in particular terpenoids, has largely remained an open question. Yet, knowledge on the natural product background is critical to understand if other compounds modulate the psychotropic pharmacological effects. CubA, the single clade II sesquiterpene synthase of P. cubensis, was heterologously produced in Escherichia coli and characterized in vitro, complemented by in vivo product formation assays in Aspergillus niger as a heterologous host. Extensive GC-MS analyses proved a function as multi-product synthase and, depending on the reaction conditions, cubebol, β-copaene, δ-cadinene, and germacrene D were detected as the major products of CubA. In addition, mature P. cubensis carpophores were analysed chromatographically which led to the detection of β-copaene and δ-cadinene. Enzymes closely related to CubA are encoded in the genomes of various Psilocybe species. Therefore, our results provide insight into the metabolic capacity of the entire genus.     

Targeting the Central Pocket of the Pseudomonas aeruginosa Lectin LecA

Pseudomonas aeruginosa is an opportunistic ESKAPE pathogen that produces two lectins, LecA and LecB, as part of its large arsenal of virulence factors. Both carbohydrate-binding proteins are central to the initial and later persistent infection processes, i. e. bacterial adhesion and biofilm formation. The biofilm matrix is a major resistance determinant and protects the bacteria against external threats such as the host immune system or antibiotic treatment. Therefore, the development of drugs against the P. aeruginosa biofilm is of particular interest to restore efficacy of antimicrobials. Carbohydrate-based inhibitors for LecA and LecB were previously shown to efficiently reduce biofilm formations. Here, we report a new approach for inhibiting LecA with synthetic molecules bridging the established carbohydrate-binding site and a central cavity located between two LecA protomers of the lectin tetramer. Inspired by in silico design, we synthesized various galactosidic LecA inhibitors with aromatic moieties targeting this central pocket. These compounds reached low micromolar affinities, validated in different biophysical assays. Finally, X-ray diffraction analysis revealed the interactions of this compound class with LecA. This new mode of action paves the way to a novel route towards inhibition of P. aeruginosa biofilms.