Supplemental inulin does not enhance iron bioavailability to Caco-2 cells from milk- or soy-based,probiotic-containing,yogurts but incubation at 37 °C does

The in vitro effects of supplemental inulin (4%) on iron (Fe) availability in two different probiotic-containing yogurts were examined. Milk or soy-based yogurts, with and without inulin, were incubated (37 °C) for 48 h or without any incubation before comparison by an in vitro gastrointestinal digestion/Caco-2 cell culture model was used to assess iron bioavailability. The dialysable Fe fraction, cell ferritin formation, and cell associated Fe were monitored. Supplemental inulin decreased dialysable Fe only in non-incubated milk-based yogurt. In both yogurts incubation by itself increased dialysable Fe, and inulin increased the latter only in soy-based yogurt. Cellular ferritin concentration were higher after exposure to non-incubated milk-based than soy-based yogurt, although, after incubation the latter induced the highest ferritin formation. These data suggest that inulin does not have a direct effect on Fe bioavailability in the small intestine, and that probiotic bacteria play an enhancing role on Fe bioavailability.     

Mixed mode failure criteria for brittle elastic V-notched structures

Three mixed mode failure initiation criteria at reentrant corners in brittle elastic materials are examined. Prediction of failure load and crack initiation angle are compared to experimental observations carried out on PMMA (polymer) and MACOR (glass ceramic) V-notched specimens. Since the mode mixity ratio influences greatly both the failure load and crack initiation angle, a detailed experimental procedure has been followed, focusing on obtaining a wide range of mode mixity ratios. It is demonstrated that by assuming a sharp V-notch tip some failure criteria predict reasonably well both the crack initiation angle and failure load.     

Mesoscale Nanostructures as a Bridge Between Homogeneous and Heterogeneous Catalysis

The environment that surrounds catalytically active metallic nanoclusters has an important role in tuning their catalytic reactivity and selectivity and can initiate novel reaction routes. In this review we will demonstrate two different approaches for utilization of the environment in bi-functional, mesoscale catalysts. In these catalytic systems, the molecules that surround the metallic nanoclusters have an active role as co-catalysts. In the first bi-functional system, the steric effects of metal-adsorbed ligands have been exploited for regulating the adsorption orientation of reactants. By favoring specific orientation of the reactants, the products selectivity was widely tuned. In the second bi-functional catalytic system, the catalytic properties of the metallic nanoclusters were controlled by their encapsulation within a polymeric matrix. The oxidation state, catalytic reactivity and stability of metallic nanoclusters were tuned by their encapsulation in polyamidoamine dendrimer molecules. Oxidation of dendrimer-encapsulated nanoclusters into highly oxidized metal-ions activated the catalyst toward a variety of reactions which were previously catalyzed by homogeneous catalysts. Moreover, by modifying the properties of the polymeric matrix, enhanced chemo-, diastereo- and enantio-selectivity were obtained. These two examples of mesoscale catalysts indicate the important role of the surrounding environment in tuning the catalytic reactivity and selectivity. In addition, it is demonstrated that these catalysts can function as a bridge between homogeneous and heterogeneous catalysis.     

Thermal healing of the sub-surface damage layer in sapphire

The sub-surface damage layer formed by mechanical polishing of sapphire is known to reduce the mechanical strength of the processed sapphire and to degrade the performance of sapphire based components. Thermal annealing is one of the methods to eliminate the sub-surface damage layer. This study focuses on the mechanism of thermal healing by studying its effect on surface topography of a- and c-plane surfaces, on the residual stresses in surface layers and on the thickness of the sub-surface damage layer. An atomically flat surface was developed on thermally annealed c-plane surfaces while a faceted roof-top topography was formed on a-plane surfaces. The annealing resulted in an improved crystallographic perfection close to the sample surface as was indicated by a noticeable decrease in X-ray rocking curve peak width. Etching experiments and surface roughness measurements using white light interferometry with sub-nanometer resolution on specimens annealed to different extents indicate that the sub-surface damage layer of the optically polished sapphire is less than 3 μm thick and it is totally healed after thermal treatment at 1450 °C for 72 h.     

Failure initiation at a blunt V-notch tip under mixed mode loading

A criterion to predict crack onset at a sharp V-notch tip in homogeneous brittle materials under a mixed-mode loading was presented and validated by experimental observations in a previous paper by the authors. This criterion slightly underestimates the experimental loads causing failure which is attributed to a small notch tip radius that blunts the sharp corner. This discrepancy is rigorously analyzed mathematically in this paper by means of matched asymptotics involving 2 small parameters: a micro-crack increment length and the notch tip radius. A correction is brought to the initial prediction and a better agreement is obtained with experiments on PMMA notched specimens.     

Oral exposure to polystyrene nanoparticles affects iron absorption

The use of engineered nanoparticles in food and pharmaceuticals is expected to increase, but the impact of chronic oral exposure to nanoparticles on human health remains unknown. Here, we show that chronic and acute oral exposure to polystyrene nanoparticles can influence iron uptake and iron transport in an in vitro model of the intestinal epithelium and an in vivo chicken intestinal loop model. Intestinal cells that are exposed to high doses of nanoparticles showed increased iron transport due to nanoparticle disruption of the cell membrane. Chickens acutely exposed to carboxylated particles (50?nm in diameter) had a lower iron absorption than unexposed or chronically exposed birds. Chronic exposure caused remodelling of the intestinal villi, which increased the surface area available for iron absorption. The agreement between the in vitro and in vivo results suggests that our in vitro intestinal epithelium model is potentially useful for toxicology studies.     

WIP Remodeling Actin behind the Scenes: How WIP Reshapes Immune and Other Functions

Actin polymerization is a fundamental cellular process regulating immune cell functions and the immune response. The Wiskott-Aldrich syndrome protein (WASp) is an actin nucleation promoting factor, which is exclusively expressed in hematopoietic cells, where it plays a key regulatory role in cytoskeletal dynamics. WASp interacting protein (WIP) was first discovered as the binding partner of WASp, through the use of the yeast two hybrid system. WIP was later identified as a chaperone of WASp, necessary for its stability. Mutations occurring at the WASp homology 1 domain (WH1), which serves as the WIP binding site, were found to cause the Wiskott-Aldrich syndrome (WAS) and X-linked thrombocytopenia (XLT). WAS manifests as an immune deficiency characterized by eczema, thrombocytopenia, recurrent infections, and hematopoietic malignancies, demonstrating the importance of WIP for WASp complex formation and for a proper immune response. WIP deficiency was found to lead to different abnormalities in the activity of various lymphocytes, suggesting differential cell-dependent roles for WIP. Additionally, WIP deficiency causes cellular abnormalities not found in WASp-deficient cells, indicating that WIP fulfills roles beyond stabilizing WASp. Indeed, WIP was shown to interact with various binding partners, including the signaling proteins Nck, CrkL and cortactin. Recent studies have demonstrated that WIP also takes part in non immune cellular processes such as cancer invasion and metastasis, in addition to cell subversion by intracellular pathogens. Understanding of numerous functions of WIP can enhance our current understanding of activation and function of immune and other cell types.