Carbonaceous Materials-12: a Novel Highly Sensitive Graphene Oxide-Based Carbon Electrode: Preparation,Characterization, and Heavy Metal Analysis in Food Samples

Graphene oxide (GO) was covalently attached to glassy carbon (GC) electrode (GC–O–GO) for fabricating nanosensors to determine trace Pb²⁺ and Cd²⁺ using differential pulse anodic stripping voltammetry (DPASV). Surface characterization of the nanofilm-covered electrode was performed via electrochemical cyclic voltammetry (CV), transmission electron microscopy (TEM), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) techniques. Surface pKa of the GO covalent attached GC (GC–O–GO) was calculated via CV. Under optimal conditions, a linear response was found for Pb²⁺ and Cd²⁺ in the range from 1?×?10^?8 to 1?×?10^?12 M. The limit of detections (LODs) of Pb²⁺ and Cd²⁺ were 0.25 pM and 0.28 pM, respectively. The method shows good reproducibility, and stability was successfully applied to measure Pb²⁺ and Cd²⁺ levels in rice, soya, milk, and tap water samples, with good agreement with those obtained by the standard inductively coupled plasma optical emission spectrometry (ICP-OES) method. The method was evaluated by application with the simultaneous determination of the ions in food samples (n?=?6) using the standard addition method. The recoveries of the Pb²⁺ and Cd²⁺ were up to 98 %.     

Effects of process variables on some quality properties of meatballs semi-cooked in a continuous type ohmic cooking system

Ohmic cooking of meatballs was conducted in a continuous type ohmic cooker using different voltage gradients (15, 20 and 25 V/cm) and holding times (0, 15 and 30 s). The color and textural properties and log reductions in total microbial count of the meatballs were assessed. The effects of process variables on these responses were evaluated by linear and quadratic mathematical models. Desirability function was used to determine the optimum ohmic pre-cooking condition by considering the criteria of minimizing hardness ratio, and maximizing chewiness ratio, resilience ratio, log reduction in microbial load, outside chroma ratio, inside chroma ratio and in range of springiness, gumminess and inside L ratios. The optimum ohmic pre-cooking condition was found to be a 15.26 V/cm voltage gradient with no holding time. It is concluded that application of the optimum condition in the related ohmic system offers potential for the production of high quality and safe semi-cooked meat products.     

Comparison of co–gasification efficiencies of coal,lignocellulosic biomass and biomass hydrolysate for high yield hydrogen production

The diversity in the chemical composition of lignocellulosic feedstocks can affect the conversion technologies employed for hydrogen production. Gasification and co–gasification activities of lignocellulosic biomass, biomass hydrolysate, and coal were evaluated for hydrogen rich gas production. The hydrolysates of biomass materials showed the best performance for gasification. The results indicated that biomass hydrolysates obtained from lignocellulosic biomass were more sensitive to degradation and therefore, produced more hydrogen and gaseous products than that of lignocellulosic biomass. The effects of feed (kenaf and sorghum hydrolysate), flow rate (0.3–2.0 mL/min) and temperature (700–900 °C) on hydrogen production and gasification yields were investigated. It was observed that 0.5 mL/min the optimum feed flow rate for the maximum total gas and hydrogen production. Synergism effects were observed for co–gasification of coal/biomass and coal/biomass hydrolysate. In all co–gasification processes, the main component of the gas mixture was hydrogen (≥70%).     

The effect of experimental stress on sarcoplasmic reticulum Ca(2+) transport and meat quality in pig muscle

Stress before slaughter affects the meat quality of pigs and a disorder in the Ca(2+) transport of the sarcoplasmic reticulum (SR) results in inferior meat quality. The object of this work was to determine the impact of stress on SR Ca(2+) transport in pig muscle in animals with no mutation in the Ca(2+) release channel. At about 80 kg live weight, pigs were stressed either by running for 4 min (n=9) or by a 5 min application of a nose snare (n=7). Immediately before and after the application of the stress, as well as 4 h after the stress, biopsy samples of the longissimus muscle were taken and the Ca(2+) uptake of the homogenised muscle samples determined. The nose snare stress reduced the rate of Ca(2+) uptake by 9% (P=0.02) and running by 18% (P=0.06). Uptake in samples taken 4 h after the stress was at initial levels. The pigs were slaughtered at an average live weight of 109 kg. Half the pigs were stressed just before slaughter for 5 min by the use of a nose snare. Immediately after exsanguination longissimus muscle samples were taken, Ca(2+) uptake measured, and later standard meat quality parameters were determined. Although the stress reduced the Ca(2+) uptake rate (61.3 vs. 46.4 nM/min per mg protein for control and stressed pigs; P=0.04), the meat quality was not significantly affected.     

Effect of manufacturing conditions on the corrosion resistance behavior of metallic bipolar plates in proton exchange membrane fuel cells

Metallic bipolar plates are one of the promising alternatives to the graphite bipolar plates in proton exchange membrane fuel cell (PEMFC) systems. In this study, stainless steel (SS304, SS316L, and SS430), nickel (Ni 270), and titanium (Grade 2 Ti) plates with an initial thickness of 51 μm were experimented as bipolar plate substrate materials in corrosion resistance tests. In addition to unformed blanks, SS316L plates were formed with stamping and hydroforming processes to obtain bipolar plates under different process conditions (stamping force, hydroforming pressure, stamping speed, hydroforming pressure rate). These bipolar plates, then, were subjected to corrosion tests, and the results were presented and discussed in detail. Potentiodynamic polarizations were performed to observe corrosion resistance of metallic bipolar plates by simulating the anodic and cathodic environments in the PEMFC. In order to determine the statistical significance of the corrosion resistance differences between different manufacturing conditions, analysis of variance (ANOVA) technique was used on the corrosion current density (I_corr, μA cm⁻²) values obtained from experiments. ANOVA for the unformed substrate materials indicated that SS430 and Ni have less corrosion resistance than the other substrate materials tested. There was a significant difference between blank (unformed) and stamped SS316L plates only in the anodic environment. Although there was no noteworthy difference between unformed and hydroformed specimens for SS316L material, neither of these materials meet the Department of Energy‘s (DOE) target corrosion rate of ≤1 μA cm⁻² by 2015 without coating. Finally, stamping parameters (i.e. speed and force levels) and hydroforming parameters (i.e. the pressure and pressure rate) significantly affected the corrosion behavior of bipolar plates.     

Experimental investigations on the corrosion resistance characteristics of coated metallic bipolar plates for PEMFC

Bipolar plates (BPPs) made of stainless steels preferred in PEM Fuel Cell (PEMFC) applications due to their high electrical conductivity, low material and production costs, low weight and mechanical strength. However, their corrosion resistances are not at desired levels for real PEMFC working conditions. To overcome this issue, different coating types are suggested. In this study, corrosion resistance behavior of 51 μm-thick SS316L metallic bipolar plates that were coated with the three different PVD coatings (TiN, CrN, and ZrN) at three thicknesses (0.1 μm, 0.5 μm, and 1 μm), and then were formed with two different manufacturing processes (stamping and hydroforming) investigated. Potentiodynamic and potentiostatic corrosion experiments were performed on the coated-formed SS316L plates, and coated-unformed blanks. Corrosion test results indicate that 1 μm ZrN coating demonstrated the highest corrosion resistance among the tested cases regardless of the manufacturing process employed. Moreover, hydroformed bipolar plates exhibited higher corrosion resistance than the stamped BPPs, but lower than the blank samples. Hardness measurements were also performed on the coated samples and resulted in higher corrosion resistance for harder surfaces.     

Computational Model for Cyclic Mobility and Associated Shear Deformation

In saturated clean medium-to-dense cohesionless soils, liquefaction-induced shear deformation is observed to accumulate in a cycle-by-cycle pattern (cyclic mobility). Much of the shear strain accumulation occurs rapidly during the transition from contraction to dilation (near the phase transformation surface) at a nearly constant low shear stress and effective confining pressure. Such a stress state is difficult to employ as a basis for predicting the associated magnitude of accumulated permanent shear strain. In this study, a more convenient approach is adopted in which the domain of large shear strain is directly defined by strain space parameters. The observed cyclic shear deformation is accounted for by enlargement and/or translation of this domain in deviatoric strain space. In this paper, the model formulation details involved are presented and discussed. A calibration phase is also described based on data from laboratory sample tests and dynamic centrifuge experiments (for Nevada sand at a relative density of about 40%).     

Conditions and chemical reaction mechanisms by which nitrosamines may be formed in biological products with reference to their possible occurrence in food products

Summary Experiments have shown that Formation of volatile nitrosamines occurs when nitrite and alkylamines are present in the same media. Heterocyclic amines such as pyrrolidine and piperidine contribute to nitrosamine Formation as well. Precursor substances leading to nitrosamine Formation are, besides various biogenic amines, certain amino acids and proteins from meat and Fish if nitrite is present in sufficient quantities and high temperature treatment is used. In order to prevent the Formed nitrosamines From escaping at high temperatures ä protecting colloid must be present. The amount and type of nitrosamine Formed under various experimental conditions have been studied by quantitative and qualitative methods. In cases where large amounts of nitrosamines were Formed, the toxicity of these was demonstrated with mink as test animals.

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Zusammenfassung Untersuchungen haben ergeben, daß die Bildung Flüchtiger Nitrosamine auftritt, wenn Nitrite und Alkylamine in demselben Stoff vorhanden sired. Ebenso tragen heterozyklische Amine wie Pyrrolidin und Piperidin zur Bildung von Nitrosaminen bei. Vorläufer der Nitrosaminbildung sind neben verschiedenen biogenischen Aminen bestimmte Aminosäuren und Proteine aus Fleisch und Finch, vorausgesetzt Nitrit ist in ausreichender Menge vorhanden und vorausgesetzt, daß hohe Erhitzungstemperaturen angewendet wurden. Um das Entweichen von gebildeten Nitrosaminen bei hohen Temperaturen zu vermeiden, muß eine Schutzhülle verwendet werden. Mit Hilfe quantitativer und qualitativer Methoden wurden die Menge und die Art der unter verschieden experimentellen Bedingungen auftretenden Nitrosaminen untersucht. Bei Auftreten stärkerer Mengen an Nitrosaminen wurde deren toxicität an Versuchstieren nachgewiesen.

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Revised version of lecture given at ä WHO meeting on nitrosamines in London, Oct. 1969.

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The authors wish to express their most sincere thanks to Docent A. Helgebostad For his valuable help by carrying out toxicological studies on mink.     

Instrumenting and tuning dataView—a networked application for navigating through large scientific datasets

dataView is an application that allows scientists to fly visually through large, regularly‐gridded, time‐varying 3D datasets from their desktop computers. dataView works with data that has been divided into cubes and sub‐cubes (which we call ‘tiles’ and ‘subtiles’), sampled at three levels of detail and written to a terabyte data server built on a PC cluster. dataView is a networked application. The dataView client component that runs on the scientist's computer is used only for user interaction and rendering. The selection of data subtiles for any given scene, and the geometry computation performed on those subtiles to create the virtual world, are performed by dataView components run in parallel on nodes of the PC cluster. This paper describes how we instrumented and tuned the code for improved performance in a networked environment. We report on how we measured network performance, first by inducing network delay and then by running the dataView client component in Washington DC and the compute components in Los Angeles. We report on the effect that tile size, level of detail, and client CPU speed have on performance. We analyze what happens when the geometry computation is performed in parallel using MPI (Message Passing Interface) vs. in serial, and discuss the effect on performance of adding additional computational nodes. Copyright © 2001 John Wiley & Sons, Ltd.