Separating consciously controlled and automatic influences in memory for new associations.

The process dissociation paradigm was applied to investigate the contributions of automatic and consciously controlled processes to the repetition priming effect for new associations, under elaborative encoding (Experiments 1 and 2) and copy instructions (Experiment 3). Semantically unrelated context–target word pairs were presented during study, and context words and stems were presented during test. Target word stems were paired with the same context words as at study (intact), paired with different context words from study (recombined), or were the stems of unstudied words (control). Participants had to complete stems with the first word that came to mind (indirect), with studied words (inclusion), or with new, unstudied words (exclusion). Results indicated that consciously controlled processes mediated the associative repetition effect under elaborative encoding, whereas automatic processes were implicated under copy instructions. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Minimizing Locality of One-Way Functions via Semi-private Randomized Encodings

A one-way function is d-local if each of its outputs depends on at most d input bits. In Applebaum et al. (SIAM J Comput 36(4):845–888, 2006), it was shown that, under relatively mild assumptions, there exist 4-local one-way functions (OWFs). This result is not far from optimal as it is not hard to show that there are no 2-local OWFs. The gap was partially closed in Applebaum et al. (2006) by showing that the existence of 3-local OWFs is implied by the intractability of decoding a random linear code (or equivalently the hardness of learning parity with noise). In this note we provide further evidence for the existence of 3-local OWFs. We construct a 3-local OWF based on the assumption that a random function of (arbitrarily large) constant locality is one-way. [A closely related assumption was previously made by Goldreich (Studies in Complexity and Cryptography. Miscellanea on the Interplay between Randomness and Computation, pp. 76–87, 2011).] Our proof consists of two steps: (1) we show that, under the above assumption, Random Local Functions remain hard to invert even when some information on the preimage x is leaked and (2) such “robust” local one-way functions can be converted to 3-local one-way functions via a new construction of semi-private randomized encoding. We believe that these results may be of independent interest.     

Gene Variant of Barrier to Autointegration Factor 2 (Banf2w) Is Concordant with Female Determination in Cichlids

Oreochromis fishes exhibit variability of sex-determination (SD) genes whose characterization contributes to understanding of the sex differentiation network, and to effective tilapia farming, which requires all-male culture. However, O. niloticus (On) amh is the only master-key regulator (MKR) of SD that has been mapped (XY/XX SD-system on LG23). In O. aureus (Oa), LG3 controls a WZ/ZZ SD-system that has recently been delimited to 9.2 Mbp, with an embedded interval rich with female-specific variation, harboring two paics genes and banf2. Developing genetic markers within this interval and using a hybrid Oa stock that demonstrates no recombination repression in LG3, we mapped the critical SD region to 235 Kbp on the orthologous On physical map (p < 1.5 × 10⁻²⁶). DNA-seq assembly and peak-proportion analysis of variation based on Sanger chromatograms allowed the characterization of copy-number variation (CNV) of banf2. Oa males had three exons capable of encoding 90-amino-acid polypeptides, yet in Oa females, we found an extra copy with an 89-amino-acid polypeptide and three non-conservative amino acid substitutions, designated as banf2w. CNV analysis suggested the existence of two to five copies of banf2 in diploidic Cichlidae. Disrupting the Hardy–Weinberg equilibrium (p < 4.2 × 10⁻³), banf2w was concordant with female determination in Oa and in three cichlids with LG3 WZ/ZZ SD-systems (O. tanganicae, O. hornorum and Pelmatolapia mariae). Furthermore, exclusive RNA-seq expression in Oa females strengthened the candidacy of banf2w as the long-sought LG3 SD MKR. As banf genes mediate nuclear assembly, chromatin organization, gene expression and gonad development, banf2w may play a fundamental role inducing female nucleus formation that is essential for WZ/ZZ SD.     

Toward a redefinition of implicit memory: Process dissociations following elaborative processing and self-generation.

Does conceptual processing affect unconscious uses of memory? The authors used a process-dissociation procedure to separate automatic (unconscious) and consciously controlled uses of memory in a stem-completion task. Contrary to results from indirect test conditions, estimates derived from the procedure showed no effect of self-generation and no differential effect of semantic and nonsemantic study conditions on automatic uses of memory. These results provide evidence that (1) indirect tests are often contaminated by conscious uses of memory and (2) stem completion is highly dependent on prior perceptual (and perhaps lexical) processing. The experiments demonstrate the advantages of using process-dissociation procedures over comparisons between direct and indirect tests. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Eradication of Multi‐Drug Resistant Bacteria by a Novel Zn‐doped CuO Nanocomposite

Zinc‐doped copper oxide nanoparticles are synthesized and simultaneously deposited on cotton fabric using ultrasound irradiation. The optimization of the processing conditions, the specific reagent ratio, and the precursor concentration results in the formation of uniform nanoparticles with an average size of ≈30 nm. The antibacterial activity of the Zn‐doped CuO Cu_0.88Zn_0.12O in a colloidal suspension or deposited on the fabric is tested against Escherichia coli (Gram negative) and Staphylococcus aureus (Gram positive) bacteria. A substantial enhancement of 10 000 times in the antimicrobial activity of the Zn–CuO nanocomposite compared to the pure CuO and ZnO nanoparticles (NPs) is observed after 10 min exposure to the bacteria. Similar activities are observed against multidrug‐resistant bacteria (MDR), (i.e., Methicillin‐resistant S. aureus and MDR E. coli) further emphasizing the efficacy of this composite. Finally, the mechanism for this enhanced antibacterial activity is presented.     

Bioenergetic and Metabolic Impairments in Induced Pluripotent Stem Cell-Derived Cardiomyocytes Generated from Duchenne Muscular Dystrophy Patients

Duchenne muscular dystrophy (DMD) is caused by mutations in the dystrophin gene and dilated cardiomyopathy (DCM) is a major cause of morbidity and mortality in DMD patients. We tested the hypothesis that DCM is caused by metabolic impairments by employing induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) generated from four DMD patients; an adult male, an adult female, a 7-year-old (7y) male and a 13-year-old (13y) male, all compared to two healthy volunteers. To test the hypothesis, we measured the bioenergetics, metabolomics, electrophysiology, mitochondrial morphology and mitochondrial activity of CMs, using respirometry, LC–MS, patch clamp, electron microscopy (EM) and confocal microscopy methods. We found that: (1) adult DMD CMs exhibited impaired energy metabolism and abnormal mitochondrial structure and function. (2) The 7y CMs demonstrated arrhythmia-free spontaneous firing along with “healthy-like” metabolic status, normal mitochondrial morphology and activity. In contrast, the 13y CMs were mildly arrhythmogenic and showed adult DMD-like bioenergetics deficiencies. (3) In DMD adult CMs, mitochondrial activities were attenuated by 45–48%, whereas the 7y CM activity was similar to that of healthy CMs. (4) In DMD CMs, but not in 7y CMs, there was a 75% decrease in the mitochondrial ATP production rate compared to healthy iPSC-CMs. In summary, DMD iPSC-CMs exhibit bioenergetic and metabolic impairments that are associated with rhythm disturbances corresponding to the patient’s phenotype, thereby constituting novel targets for alleviating cardiomyopathy in DMD patients.     

Mode I interlaminar fracture toughness of Nylon 66 nanofibrilmat interleaved carbon/epoxy laminates

Carbon/epoxy laminates interleaved with laboratory scale electrospun Nylon 66 nanofibrilmat and spunbonded nonwoven mats were investigated. The effect of the nanoscale fibers on the fracture toughness of the composite under pure Mode I loading was evaluated. It was shown that the nanofibrilmat is responsible for a major interlaminar fracture toughness improvement, as high as 255–322%, compared to a noninterleaved carbon/epoxy reference laminate. We further studied the improvement mechanism of the electrospun nanofibrilmat compared to a commercial spunbonded nonwoven Nylon 66 mat. A combination of two interlayer fracture mechanisms responsible for the toughness improvement is suggested: the first is related to the high energy dissipated by bridged thermoplastic nanofibers and the second is attributed to the generation of a plastic zone near the crack tip. The interlaminar fracture mechanisms of both electrospun nanofibrilmat and the nonwoven mat interleaving was analyzed and discussed. POLYM. COMPOS., 2011. © 2011 Society of Plastics Engineers