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71.
72.
de Oliveira Neto Roberto Evaristo Cartaxo Juliana de Melo Rodrigues Alisson Mendes Barros Smea Valensca Alves da Costa Fabiana Pereira Neves Gelmires de Arajo Menezes Romualdo Rodrigues 《Clean Technologies and Environmental Policy》2022,24(5):1403-1415
Clean Technologies and Environmental Policy - Powdered and coarse perlite wastes (PP and CP) were used to develop new sustainable mortar compositions. The as-received PP and CP wastes were... 相似文献
73.
Irene L. Gutirrez Fabiana Novellino Javier R. Caso Borja García-Bueno Juan C. Leza Jos L. M. Madrigal 《International journal of molecular sciences》2022,23(6)
The chemokine CCL2 participates in multiple neuroinflammatory processes, mainly through the recruitment of glial cells. However, CCL2 has also been proven to exert different types of actions on these cells, including the modification of their response to inflammatory stimuli. In the present study we analyzed the effect of CCL2 on the resolution of inflammation in astrocytes. We observed that genetic removal of CCL2 increases the expression of the enzymes responsible for the synthesis of specialized pro-resolving mediators arachidonate 15-lipoxygenase and arachidonate 5-lipoxygenase in the brain cortex of 5xFAD mice. The expression of FPR2 receptor, known to mediate the activity of pro-resolving mediators was also increased in mice lacking CCL2.The downregulation of these proteins by CCL2 was also observed in cultured astrocytes. This suggests that CCL2 inhibition of the resolution of inflammation could facilitate the progression of neuroinflammatory processes. The production of the pro-inflammatory cytokine IL-1beta by astrocytes was analyzed, and allowed us to confirm that CCL2 potentiates the activation of astrocytes trough the inhibition of pro-resolving pathways mediated by Resolvin D1. In addition, the analysis of the expression of TNFalpha, MIP1alpha and NOS2 further confirmed CCL2 inhibition of inflammation resolution in astrocytes. 相似文献
74.
Marco Biondi Laura Indolfi Francesca Ungaro Fabiana Quaglia Maria Immacolata La Rotonda Paolo A. Netti 《Journal of materials science. Materials in medicine》2009,20(10):2117-2128
In tissue engineering, the recapitulation of natural sequences of signaling molecules, such as growth factors, as occurring
in the native extracellular matrix (ECM), is fundamental to support the stepwise process of tissue regeneration. Among the
manifold of tissue engineering strategies, a promising one is based on the creation of the chrono-programmed presentation
of different signaling proteins. This approach is based upon the integration of biodegradable microspheres, loaded with suitable
protein molecules, within scaffolds made of collagen and, in case, hyaluronic acid, which are two of the fundamental ECM constituents.
However, for the design of bioactivated gel-like scaffolds the determination of release kinetics must be performed directly
within the tissue engineering template. In this work, biodegradable poly(lactic-co-glycolic)acid (PLGA) microspheres were
produced by the multiple emulsion-solvent evaporation technique and loaded with rhodamine-labelled bovine serum albumin (BSA-Rhod),
a fluorescent model protein. The microdevices were dispersed in collagen gels and collagen-hyaluronic acid (HA) semi-interpenetrating
networks (semi-IPNs). BSA-Rhod release kinetics were studied directly on single microspheres through confocal laser scanning
microscopy (CLSM). To thoroughly investigate the mechanisms governing protein release from PLGA microspheres in gels, BSA-Rhod
diffusion in gels was determined by fluorescence correlation spectroscopy (FCS), and water transport through the microsphere
bulk was determined by dynamic vapor sorption (DVS). Moreover, the decrease of PLGA molecular weight and glass transition
temperature (Tg) were determined by gel permeation chromatography (GPC) and differential scanning calorimetry (DSC), respectively. Results
indicate that protein release kinetics and delivery onset strongly depend on the complex interplay between protein transport
through the PLGA matrix and in the collagen-based release media, and water sequestration within the scaffolds, related to
the scaffold hydrophilicity, which is dictated by HA content. The proper manipulation of all these features may thus allow
the obtainment of a fine control over protein sequential delivery and release kinetics within tissue-engineering scaffolds. 相似文献
75.
Lutein is an oxygenated carotenoid (xanthophyll) found in dark green leafy vegetables. High intakes of lutein may lower the
risk of age-related macular degeneration. Current understanding of human lutein metabolism as it might occur in vivo is incomplete. Therefore, we conducted a feasibility study where we dosed a normal adult woman with 14C-lutein (125 nmol, 36 nCi 14C), dissolved in olive oil (0.5 g/kg body weight) and mixed in a banana shake. Blood, urine, and feces collected before the
dose was administered served to establish baseline values. There-after, blood was collected for 63 d following the dose, while
feces and urine were collected for 2 wk post-dose. The 14C contents in plasma, urine, and feces were measured by accelerator MS. The 14C first appeared in plasma 1 h after dosing and reached its highest level,≈2.08% of dose/L plasma, at 14 h post-dose. The
plasma pattern of 14C did not include a chylomicrons/VLDL (intestinal) peak like that when the same subject received 14C-β-carotene (a previous test), suggesting that lutein was handled differently from β-carotene by plasma lipoproteins. Lutein
had an elimination half-life (t
1/2) of≈10 d. Forty-five percent of the dose of 14C was eliminated in feces and 10% in urine in the first 2 d after dosing. Quantifying human lutein metabolism is a fertile
area for future research. 相似文献
76.
Vanessa Rios de Souza Patrícia Aparecida Pimenta Pereira Ana Carla Marques Pinheiro Luiz Carlos de Oliveira Lima Rafael Pio Fabiana Queiroz 《Journal of food science》2014,79(9):S1776-S1781
In Brazil, there are numerous blackberry cultivars under cultivation, however, only a few cultivars, such as the Guarani, are displayed/used for processing. In this context the aim of this work was to study the effects of different Brazilian blackberry cultivars (Guarani, Brazos, Comanche, Tupy, Cherokee, Caingangue, and Choctaw) on the physicochemical characteristics, texture profiles and the consumer acceptance of the resulting jelly to identify the potential use of these cultivars in the jelly industry. It is feasible to produce blackberry jellies with the Tupy, Comanche, Brazos, Guarani, and Choctaw cultivars because these jellies demonstrated good acceptability combined with good productivity. Consumers were shown to have a preference for a less adhesive, more acidic jelly. This study provides a useful insight about the Brazilian blackberry cultivar potential in jelly processing. 相似文献
77.
Romina L. Abarca Francisco J. Rodríguez Abel Guarda María J. Galotto Julio E. Bruna Mary A. Fávaro Perez Fabiana Ramos Souza Felipe Marisa Padula 《Food and Bioprocess Technology》2017,10(9):1585-1594
The aim of this study was to develop, characterize and evaluate in vitro the efficacy of active films, based on an inclusion complex formed by β-cyclodextrin, 2-nonanone and two polymer matrices (polylactic acid and low density polyethylene). The different films were characterized by scanning electronic microscopy (SEM), differential scanning calorimetry (DSC), themogravimetric analysis (TGA), optical properties and antimicrobial activity against B. cinerea. The results showed important differences in the parameters evaluated where the level of agglomerates of additives was a key to explain these changes. Finally, microbiological analysis showed high effectiveness in reducing the Botrytis cinerea growth. The active films developed in this study were able to inhibit the growth of phytopathogenic fungus B. cinerea at different experimental conditions. The studied films have potential use for packaging fresh fruit susceptible to biological attack by this fungus. 相似文献
78.
Fabiana A. Curylofo‐Zotti Maria P. Fernandes Amanda A. Martins Ana Paula Macedo Lucas Fabricio B. Nogueira Ana Paula Ramos Silmara A. M. Corona 《Microscopy research and technique》2020,83(2):133-139
This study aimed to investigate dentin wettability and surface morphology after selective removal of carious lesion by erbium‐doped yttrium aluminum garnet (Er:YAG) laser, followed by dentin biomodification with carbodiimide (EDC) and chitosan (CHI). Seventy‐eight bovine dentin specimens were submitted to caries induction. Specimens were distributed according to methods of carious removal (n = 39): bur at low‐speed (40,000 rpm) or Er:YAG laser (noncontact mode, 250 mJ/pulse and 4Hz). All specimens were etched with 35% phosphoric acid, and subdivided according to dentin biomodification (n = 13): Control (no biomodification), EDC or CHI. The contact angle (n = 10) between adhesive system (3M ESPE) and dentin surface was measured by a goniometer. Eighteen specimens (n = 3) were analyzed by scanning electron microscopy. Data were analyzed by two‐way ANOVA and Tukey's test (α = .05). The method used to remove carious lesion did not influence the wettability of dentinal surface (p = .748). The angles produced on the remaining dentin after biomodification were influenced (p = .007). CHI promoted higher contact angles (p = .007) and EDC did not differ from the control group (p = .586). In the bur‐treated group, most tubules were open, regardless of which biomodifier was used. Laser modified the organic matrix layer. CHI promoted partially closed tubules in some areas while EDC exposed dentinal tubules. Regardless of which method was used for selective removal of carious lesion, biomodification with EDC did not affect the dentin wettability, whereas CHI changed the wettability of remaining dentin. Both biomodifiers promoted a slight change on dentin morphology. 相似文献
79.
Preference ranking of colour in raw and cooked shrimps 总被引:1,自引:0,他引:1
Jane Parisenti Luiz H. Beirão Vera L. C. G. Tramonte Fabiana Ourique Camila C. da Silveira Brito Caroline Camila Moreira 《International Journal of Food Science & Technology》2011,46(12):2558-2561
The reddish colour to shrimp, which has been associated with its high quality, is one of the major factors for its acceptability by consumers. The aim of this work was to analyse consumer preference regarding colour in raw and cooked shrimps. Farmed shrimps Litopenaeus vannamei (13.8 + 1.16 g) were fed with control or supplemented food (60 ppm of carotenoids). The shrimp samples were analysed in naked eye, confirmed by colorimetry and sorted in the following categories: dark grey (L* 27.99; h 168.16), grey (L* 32.58; h 124.60), and light grey (L* 36.2; h 119.35) for raw shrimps; and intense orange (L* 61.49; h 54.69), orange (L* 65.97; h 57.79) and light orange (L* 72.65; h 70.17) for cooked shrimps. A preference ranking test was performed with 35 judges invited to rank the samples in descending order of preference. Consumers prefer lighter raw shrimp (light grey and grey) and brightly orange cooked shrimps (orange and intense orange), which indicates that supplementing shrimp food for pigmentation should be followed by improving its acceptance by consumer. These results indicate a challenge for the shrimp industry, as the lightest raw shrimps are also not so brightly orange coloured after cooking. 相似文献
80.
Julián Puszkiel Fabiana Gennari Pierre Arneodo Larochette Fahim Karimi Claudio Pistidda Rapee Gosalawit-Utke Julian Jepsen Torben R. Jensen Carsten Gundlach José Bellosta von Colbe Thomas Klassen Martin Dornheim 《International Journal of Hydrogen Energy》2013
The hydrogen sorption behavior of the Mg2FeH6–MgH2 hydride system is investigated via in-situ synchrotron and laboratory powder X-ray diffraction (SR-PXD), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), particle size distribution (PSD) and volumetric techniques. The Mg2FeH6–MgH2 hydride system is obtained by mechanical milling in argon atmosphere followed by sintering at high temperature and hydrogen pressure. In-situ SR-PXD results show that upon hydriding MgH2 is a precursor for Mg2FeH6 formation and remained as hydrided phase in the obtained material. Diffusion constraints preclude the further formation of Mg2FeH6. Upon dehydriding, our results suggest that MgH2 and Mg2FeH6 decompose independently in a narrow temperature range between 275 and 300 °C. Moreover, the decomposition behavior of both hydrides in the Mg2FeH6–MgH2 hydride mixture is influenced by each other via dual synergetic-destabilizing effects. The final hydriding/dehydriding products and therefore the kinetic behavior of the Mg2FeH6–MgH2 hydride system exhibits a strong dependence on the temperature and pressure conditions. 相似文献