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141.
142.
Measurement Techniques - The affiliation of the first author, E. V. Kostikova, is incorrect  相似文献   
143.
Broad substrate tolerance and excellent regioselectivity, as well as independence from sensitive cofactors have established benzoic acid decarboxylases from microbial sources as efficient biocatalysts. Robustness under process conditions makes them particularly attractive for preparative-scale applications. The divalent metal-dependent enzymes are capable of catalyzing the reversible non-oxidative (de)carboxylation of a variety of electron-rich (hetero)aromatic substrates analogously to the chemical Kolbe-Schmitt reaction. Elemental mass spectrometry supported by crystal structure elucidation and quantum chemical calculations verified the presence of a catalytically relevant Mg2+ complexed in the active site of 2,3-dihydroxybenoic acid decarboxylase from Aspergillus oryzae (2,3-DHBD_Ao). This unique example with respect to the nature of the metal is in contrast to mechanistically related decarboxylases, which generally have Zn2+ or Mn2+ as the catalytically active metal.  相似文献   
144.
Multimedia Tools and Applications - The recognition of human emotion is a significant contribution to many computer vision appli-cations. Despite its importance, this work is the first one towards...  相似文献   
145.
We report the feasibility of electrospinning of protein-polymer multilayered scaffolds with selected materials such as non-hydrolyzed silk sericin (SS), polyethylene oxide (PEO), and polylactic acid (PLA), with tuned fiber size and properties for each layer. We present a new innovative way for the electrospinning (ES) of non-hydrolyzed SS mixed with PEO yielding fibers with an average diameter ranging between 120 and 150 nm. Different SS:PEO ratios have been electrospun to study the effect of the concentration of SS protein on the fibers size and shape, as well and their electrospinnability. Electrospun SS:PEO fibers display weak to no mechanical resistance (non-measurable) and their deposition onto a sturdier scaffold is necessary to allow their use in biomedical and/or pharmaceutical fields. Therefore, bilayer scaffolds have been fabricated consisting of a PLA support and SS:PEO fibers obtained from the optimized SS:PEO ratio (1.2:4). They are composed of a sturdy hydrophobic layer of PLA fibers and a layer of sticky hydrophilic SS:PEO fibers. The scaffolds have been characterized extensively by Fourier transforms infra-red (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and their resistance to mechanical stress. Finally, hydrophobicity of both layers has been determined by measuring the contact angle of water droplets on the scaffolds, further proving the bilayer nature of the scaffolds.  相似文献   
146.
In this paper, we consider new approaches when designing video systems with multiple video streams in conformity with the technology of crystal-based video systems combining photoreceiving matrix and computer within one engineering and methodological unity. It is demonstrated by the example of solving the problem of image stabilization that crystal-based video systems with multiple video outputs make it possible to increase the accuracy and speed of image stabilization control loop on the photoreceiving matrix by many times.  相似文献   
147.
The effect of storage after irradiation of corn grains on steeping process, starch isolation, quality of by-products and fatty acids composition of germ oil was investigated. It was found that post-irradiation storage led to a decrease in water absorption and protein solubilization during steeping process. The starch yield was lower, contained a high percentage of protein and was darker from above 500 Krads. Post-irradiation caused a change in the proportions and disappearance or induced new fatty acids in the corn germ oil.  相似文献   
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