Production and cross-reactivity patterns of a panel of high affinity monoclonal antibodies to Vibrio cholerae O139 Bengal

Abnormal brain stem auditory-evoked responses (BAER) were recorded on 14 dogs with brain lesions confirmed by necropsy (n = 13) or magnetic resonance imaging and surgical biopsy (n = 1). Lesions included brain stem or cerebellar tumors (6 dogs), brain stem trauma (1 dog), forebrain tumors (3 dogs), hydrocephalus (2 dogs), granulomatous meningoencephalitis (1 dog), and meningoencephalitis (1 dog). Five affected dogs were comatose at the time of recording. BAER abnormalities could be classified as (1) absence of some or all of waves I to V, (2) increased latencies, with wave V being most frequently affected, or (3) a reduction in the amplitude ratio of waves V/I.     

Recombinant human granulocyte colony-stimulating factor (filgrastim) following high-dose chemotherapy and peripheral blood progenitor cell rescue in high-grade non-Hodgkin's lymphoma: clinical benefits at no extra cost

In order to evaluate the potential clinical and economic benefits of granulocyte colony-stimulating factor (G-CSF, filgrastim) following peripheral blood progenitor cells (PBPC) rescue after high-dose chemotherapy (HDCT), 23 consecutive patients aged less than 60 years with poor-prognosis, high-grade non-Hodgkin's lymphoma (NHL) were entered into a prospective randomized trial between May 1993 and September 1995. Patients were randomized to receive either PBPC alone (n = 12) or PBPC+G-CSF (n = 11) after HDCT with busulphan and cyclophosphamide. G-CSF (300 microg day[-1]) was given from day +5 until recovery of granulocyte count to greater than 1.0 x 10(9) l(-1) for 2 consecutive days. The mean time to achieve a granulocyte count > 0.5 x 10(9) l(-1) was significantly shorter in the G-CSF arm (9.7 vs 13.2 days; P<0.0001) as was the median duration of hospital stay (12 vs 15 days; P = 0.001). In addition the recovery periods (range 9-12 vs 11-17 days to achieve a count of 1.0 x 10(9) l[-1]) and hospital stays (range 11-14 vs 13-22 days) were significantly less variable in patients receiving G-CSF in whom the values clustered around the median. There were no statistically significant differences between the study arms in terms of days of fever, documented episodes of bacteraemia, antimicrobial drug usage and platelet/red cell transfusion requirements. Taking into account the costs of total occupied-bed days, drugs, growth factor usage and haematological support, the mean expenditure per inpatient stay was pound sterling 6500 (range pound sterling 5465-pound sterling 8101) in the G-CSF group compared with pound sterling 8316 (range pound sterling 5953-pound sterling 15,801) in the group not receiving G-CSF, with an observed mean saving of 1816 per patient (or 22% of the total cost) in the G-CSF group. This study suggests that after HDCT and PBPC rescue, the use of G-CSF leads to more rapid haematological recovery periods and is associated with a more predictable and shorter hospital stay. Furthermore, and despite the additional costs for G-CSF, these clinical benefits are not translated into increased health care expenditure.     

Controlling parameters of focused ion beam (FIB) on high aspect ratio micro holes milling

Focused ion beam (FIB) direct milling is now recognized as a new method of fabrication, due to high flexibility in milling dimensions, the possible geometries and the material selectivity. This paper discusses the fabrication of micro holes using FIB direct milling in terms of high aspect ratio structures, including FIB parameters and the major effects of FIB milling. It is deduced that sputter yield of material gives a major impact to the depth of milling. Optimization parameters coupled control of FIB direct milling will provide a precise means of fabricating of high aspect ratio micro holes whilst resolving the problem of re-deposition and amorphisation which is common in micro milling.     

Understanding bioenergy production and optimisation at the nanoscale – a review

ABSTRACT

Nanotechnology has an increasingly large impact on a wide range of biotechnological, pharmacological and pure technological applications. Its current use in bioenergy production from biomass is very limited. This paper examines the potential interrelationships between nanotechnology and bioenergy production through a comprehensive literature review and analysis of data from biomass characterisation studies. The aim of this review is to indicate how nanotechnology can be applied in biomass-to-bioenergy conversion. This study shows currently nanotechnology has been applied in the production of only two types of biomass, i.e. sludge and algae. Hence, interaction of nanomaterials with active sludge and algal cells were examined. Our extensive literature review indicates that anaerobic digestion process in sludge can potentially be enhanced by using magnetite nanoparticles, which gives higher methane yields. On the other hand, nanosilver reduces growth and causes adverse effects on the morphology of green algae. This process for bioenergy generation has already been successfully applied to sludge and algae biomass. Our study confirms that the process can also be used in the production of bioenergy from the other biomasses, such as agricultural wastes and industrial residues. Outcomes of this work will be an important tool for implementing nanotechnology in bioenergy research.     

Occlusion filling in stereo: Theory and experiments

A number of stereo matching algorithms have been developed in the last few years, which also have successfully detected occlusions in stereo images. These algorithms typically fall short of a systematic study of occlusions; they predominantly emphasize matching and regard occlusion filling as a secondary operation. Filling occlusions, however, is useful in many applications such as image-based rendering where 3D models are desired to be as complete as possible. In this paper, we study occlusions in a systematic way and propose two algorithms to fill occlusions reliably by applying statistical modeling, visibility constraints, and scene constraints. We introduce a probabilistic, model-based filling order of the occluded points to maintain consistency in filling. Furthermore, we show how an ambiguity in the interpolation of the disparity value of an occluded point can safely be avoided using color homogeneity when the point’s neighborhood consists of multiple scene surfaces. We perform a comparative study and show that statistically, the new algorithms deliver good quality results compared to existing algorithms.     

An improved facility layout construction method

This study presents a new distance-based facility layout construction technique. Given a two-dimensional (i.e. single-floor) facility layout construction problem in which the order of placement of individual departments is known (a challenging problem in itself), the technique presented herein proposes the use of sub-departments and expected distance functions instead of centroid-to-centroid distances for the placement of departments. In this paper an expected distance function is defined as the probabilistic expectation of the particular distance metric of interest (rectilinear, Euclidean, etc.) in which the parameters involved are defined by random vectors in 2-dimensional Euclidean space. This study presents an enhanced facility layout construction technique that incorporates several enhancements over the well-known systematic layout procedure (SLP). The goal herein is to minimise the error induced by the use of the centroid-to-centroid distances between the departments inherent to the SLP.     

Nanoimprint lithography for optic fluidics with phase gratings for environmental monitoring application

Optic fluidic devices, which combine optical elements such as adaptive optical lenses or diffraction gratings into micro-fluidic devices, are now becoming increasingly important for environmental monitoring, medical diagnostics and chemical detection. In this paper, we present a novel and simple micro/nanofabrication process for SU-8 based optic fluidics structures with phase gratings on the bottom of the micro-channel using our silicon grating templates. The refraction index difference between the fluid and the SU-8 gratings generates a modulation in the phase of the light passing through the device. Our method of fabricating optic fluidic device is promising to be economic in fabrication, high throughput in production and have potential applications for sensing.     

Closed form models for dwell point locations in automated storage carousel systems

This research focuses on determining the optimal dwell point location in an automated storage carousel system (ASCS) to minimise the expected response time for incoming service requests (storages and retrievals). This can be achieved by modelling the problem as a minisum location problem on a line segment with two-dimensional existing facilities (demands) using a continuous approximation of the individual storage locations of the ASCS. Closed form solutions of the associated dwell point location problem have been provided for the two different cases used to model this problem. These two cases arise from the two possible different configurations of the ASCS parameters; the travel times in circumference and height.     

High-Reliability Low-Ag-Content Sn-Ag-Cu Solder Joints for Electronics Applications

Sn-Ag-Cu (SAC) alloy is currently recognized as the standard lead-free solder alloy for packaging of interconnects in the electronics industry, and high- Ag-content SAC alloys are the most popular choice. However, this choice has been encumbered by the fragility of the solder joints that has been observed in drop testing as well as the high cost of the Ag itself. Therefore, low-Ag-content SAC alloy was considered as a solution for both issues. However, this approach may compromise the thermal-cycling performance of the solders. Therefore, to enhance the thermal-cycling reliability of low-Ag-content SAC alloys without sacrificing their drop-impact performance, alloying elements such as Mn, Ce, Ti, Bi, In, Sb, Ni, Zn, Al, Fe, and Co were selected as additions to these alloys. However, research reports related to these modified SAC alloys are limited. To address this paucity, the present study reviews the effect of these minor alloying elements on the solder joint reliability of low-Ag-content SAC alloys in terms of thermal cycling and drop impact. Addition of Mn, Ce, Bi, and Ni to low-Ag-content SAC solder effectively improves the thermal-cycling reliability of joints without sacrificing the drop-impact performance. Taking into consideration the improvement in the bulk alloy microstructure and mechanical properties, wetting properties, and growth suppression of the interface intermetallic compound (IMC) layers, addition of Ti, In, Sb, Zn, Al, Fe, and Co to low-Ag-content SAC solder has the potential to improve the thermal-cycling reliability of joints without sacrificing the drop-impact performance. Consequently, further investigations of both thermal-cycling and drop reliability of these modified solder joints must be carried out in future work.