Abrogation of p53 function affects gadd gene responses to DNA base-damaging agents and starvation

The tumor suppressor p53 is required for induction of its downstream effector genes such as GADD45 and CIP1/WAF1 by ionizing radiation (IR). This response is probably mediated through defined p53 binding sites located in the promoter of CIP1/WAF1 and in the third intron of GADD45. In contrast, the gadd gene stress response to base-damaging agents, such as methylmethane sulfonate (MMS) or UV radiation, or medium depletion (starvation) occurs in all mammalian cells examined to date regardless of p53 status for both GADD45 and also GADD153, which is not IR-responsive in many lines with functional p53. These agents strongly induce the p53 protein and raise the possibility that, although p53 is not required for the typical "gadd" response to these agents, p53 may contribute to these non-IR stress responses. This possibility was confirmed by the finding that disruption of p53 function by transfection with dominant-negative vectors expressing HPV E6, mutant p53, or SV40 T Ag reduced the induction of GADD45 and GADD153 as measured by increases in mRNA and protein levels in human lines with wild-type p53. Similarly, induction of these genes by MMS or UV radiation was consistently stronger in the parental mouse embryo fibroblasts compared to cells derived from mice where both p53 alleles had been deleted. Similar qualitative responses were also seen for CIP1/WAF1. In agreement with reduced induction of p53-regulated genes, the G1 checkpoint activated by MMS or UV radiation was markedly abrogated in p53-wt human MCF-7 breast carcinoma cells by E6 expression. Interestingly, induction of reporter constructs driven by the GADD45 or GADD153 promoters was substantially reduced in human cells transfected with mutant p53 or E6 expression vectors or in cells lacking p53 following treatment with MMS, UV radiation, or starvation. Because neither promoter is inducible by IR, and neither contains a strong p53 binding site, these results indicate that p53 has a synergistic or cooperative role in these non-IR stress responses for both GADD45 and GADD153, and that this role is not mediated through identifiable p53-binding sites.     

便携式危险气体报警器的研究与设计

通过测定瓦斯的主要成分甲烷的浓度来间接测量瓦斯浓度是解决目前我国煤矿瓦斯事故多发的有效途径;但大多数测定瓦斯浓度的设备具有体积大、难移动的缺点;针对以上情况,基于对MSP430系列单片机超低功耗和片内外设丰富的特点的深入了解和研究,设计了以甲烷为被检测气体,以MSP430F135为微控制器的便携式危险气体报警器;通过它能够实时监测矿井中甲烷的浓度,并在浓度超过报警上限值时启动蜂鸣、闪光和振动为一体的报警功能;其声、光和震动三为一体的报警功能具有鲜明的独创性和重要性。     

一种分布式原油储罐油水界面监测系统设计

针对采油厂原油储罐油水界面参数自动化测量的技术需求,在介绍电容法测量液位原理的基础上,给出了一种矩阵式分布的微电容串组结构的油水界面测量仪的设计方法,探讨了测量结果的阵列数据获取界面参数的数据分析方法,并借助RS485通信网络实现了原油储罐油水界面的分布式测量;详细阐述了该分布式测量系统的组成、通信协议和主要程序流程;实际应用结果表明,该系统测量精度可达到≤10cm,工作可靠,可以在生产过程中发挥积极作用。     

故障诊断测试系统通用报表生成接口库的设计

针对故障诊断测试系统的测试结果报表生成操作复杂、效率低下、格式难以统一的问题,文章提出了一种新的报表生成方法;设计了测试数据保存的通用数据结构,利用基于ActiveX的LabWindows/CVI与Word、Excel之间的通信技术对报表文档进行操作,生成报表并对文档中的文字和图表进行格式设置,编写了数据保存和报表生成的功能接口并封装成动态链接库,形成了规范的报表生成方案;通过使用此方法,保证了测试和数据保存的实时性,实现了统一、美观的格式控制,大大简化了测试程序的开发。     

一种基于积分投影的人眼定位方法

文章研究了人脸识别中的人眼定位这一重要环节。将经过预处理的人脸图像先通过积分投影变换法进行人脸定位,在定位后的人脸区域内用一定的矩形框搜索,给方框内黑色像素进行计数,当黑色像素个数达到某个比例时,将其标记为人眼位置侯选处。然后将小方框适当扩大,用canny算子算出人眼的边缘轮廓,并在候选位置中进一步确定人眼位置。实验结果表明本文采用的方法简单易行,具有较高的准确率和较强的光照鲁棒性。     

FlexRay总线架构下线控转向系统可靠性分析

文章在研究FlexRay总线理论基础上,对FlexRay总线式线控转向系统的可靠性进行研究,给出了具有容错结构的线控转向系统的方案。在对系统可靠性分析中采用FEMA的分析方法,有效的分析系统中的隐患;采用硬件冗余的方案设计出双通道冗余型的线控系统,大大提高FlexRay线控转向系统的可靠性要求。     

光纤到户（FTTH）的ODN工程设计及测试

文章针对PON系统的基本保护要求,提出了光分路器（OBD）设计原则;采用最坏值法对PON系统最远的用户终端,进行ODN光通道衰减核算;同时,对ODN光缆线路进行分段衰减测试和全程衰减测试,测试结果满足FTTH系统工程规范。根据现场试验的研究和测试结果,规范光纤到户（FTTH）工程系统的规划、设计、施工、监理及竣工验收等工作。     

Indeterminacy-aware service selection for reliable service composition

With the development of Internet and Web service technology, Web service composition has been an effective way to construct software applications; service selection is the crucial element in the composition process. However, the existing selection methods mostly generate static plans since they neglect the inherent stochastic and dynamic nature of Web services. As a result, Web service composition often inevitably terminates with failure. An indeterminacy-aware service selection algorithmbased on an improved Markov decision process (IMDP) has been designed for reliable service composition, but it suffers from higher computation complexity. Therefore, an efficient method is proposed, which can reduce the computation cost by converting the service selection problem based on IMDP into solving a nonhomogeneous linear equation set. Experimental results demonstrate the success rate of service composition has been improved greatly, whilst also reducing computation cost.     

Salient structural elements based texture synthesis

Salient structural elements are ubiquitous in natural textures, and their distribution exhibits some stochastic distribution features. Current texture synthesis algorithms can neither preserve the integrity of the elements nor capture this distributive information. We present an algorithm to treat this high-level visual information. Here, we address the issue by taking specific care of the structural elements. Our texture synthesis process grows the target texture one structural element at a time. A Markov ...     

云平台信息安全整体保护技术研究

目前云计算发展迅速,云平台的安全问题也应引起足够重视。本文根据云平台的结构特点,结合信息系统整体保护要求,提出了一种针对云平台的信息安全整体保护技术方案。该方案将云平台分为云计算环境,云区域边界,云通信网络以及云安全管理中心四个部分,分别对这四部分做了安全性分析,并提出了每一部分的安全保障技术设计要求。