Assay of prolactin after freezing cow's milk

Concentrations of prolactin were similar in aliquots of the same milk sample stored for 2 days at 4 C or --23 C but averaged lower if prepared for assay at 30 C than if prepared at 40 or 50 C. Average deviations in prolactin between duplicate measurements relative to respective treatment averages were lowest generally when technique of mixing included vortexing for 5 s immediately prior to pipetting volumes of milk for assay. The average deviation of differences in prolactin among duplicate measurements relative to average concentrations of prolactin were about the same for colostrum, milk, and blood plasma (11, 17, and 14%) stored frozen. Prolactin can be measured reliably in frozen cow's milk provided samples are warmed to 40 C to 50 C and mixed thoroughly when prepared for assay.     

Increased synthesis of prostaglandin-E-like material by platelets from patients with diabetes mellitus

We studied the effects of ADP, epinephrine, collagen and arachidonic acid on platelet production of immunoreactive prostaglandin-E-like material and aggregation in 17 subjects with diabetes mellitus and 21 matched controls. Plateletrich plasma obtained from patients synthesized significantly (P less than 0.05) greater quantities of the prostaglandin-E-like material after exposure to 1 muM ADP, 1, 2 and 5 muM epinephrine and 1 microgram per milliliter of collagen than platelet-rich plasma obtained from controls. That obtained from the diabetic patients was significantly more sensitive (P less than 0.001) to the aggregating effects of the prostaglandin precursor, arachidonic acid, in vitro as compared to controls. Diabetic platelet-rich plasma metabolized arachidonic acid (0.5 mM) to immunoreactive prostaglandin-E-like material at a significantly greater rate (P less than 0.05) and extent (P less than 0.001) than that of controls. Thus, platelets obtained from diabetic patients possess increased activity of the prostaglandin synthetase system, and this characteristic may be related to the increased platelet aggregation associated with the disease.     

Cryotherapy of periocular squamous cell carcinoma in the horse

Squamous cell carcinoma around the eyes of 3 horses was treated with liquid nitrogen, using cryotherapy probes as the method of application. In 2 cases, there was complete regression of the tumor; in the 3rd case, remission and relief of discomfort were temporary.     

Generation of closely spaced, doublet, CFA cathode pulses with aline-type modulator

Dual-channel, line-type modulators that have advantages in size, weight, redundancy, duty cycle, and independent pulse energy adjustment are described. The dual-channel, line-type modulators are used to generate doublet cross-field amplifier (CFA) cathode pulses in a 1.25-MW peak RF power output master oscillator power amplifier (MOPA) transmitter chain. The amplifier chain consists of a 20-kW peak power traveling-wave tube, a 150-kW peak power intermediate CFA, and a final 1.25-MW CFA stage. The intermediate CFA stage modulator (22 kV and 14 A peak) and the final CFA stage modulator (35 kV and 77 A peak) are described with equipment photographs, test data, and results. The performance requirements for each CFA stage are presented. The advantages of the doublet line-type modulator in comparison to other high-power pulse modulators are presented     

Clinical and immunological criteria of the activity of inflammatory reaction and autolymphokine therapy in penetrating wounds of the eye

Fluorometry of histamine and serotonin in cadaveric blood revealed a threefold increase of their concentrations in comparison with those in whole blood of live subjects. This indicates liberation of amines in the course of death and after it when factors inactivating them cease to function. However, the authors consider it possible to validate their conclusions about changes in the content of histamine and serotonin in cadaveric tissues in different diseases, provided certain conditions of selecting the material are adhered to. The distribution of amines in the viscera is not sex-dependent, little depends on the blood content of tissues, and is determined by the organ-specific features of metabolism of these bioactive substances. To illustrate this, the authors consider the content of histamine and serotonin in the organs of patients dead from hemorrhagic fever with the renal syndrome.