Pathway engineering for the production of aromatic compounds in Escherichia coli

The standard enzymatic assay for quantification of D-sorbitol in plasma was adapted to the automatic analyzer Cobas Mira S. In the assay, NAD (reagent) in the presence of sorbitoldehydrogenase (SDH; start reagent) converts D-sorbitol to fructose with formation of NADH, which was detected automatically as the difference between the first and last readings at 340 nm. The sample blank values for each specimen were subtracted to exclude both endogenous D-sorbitol and sugars, which also react as substrates for SDH. The method is simple, rapid (40 samples/h), precise down to endogenous concentrations (coefficient of variation < 5%; limit of determination: 0.38 mg/L) and linear up to 100 mg/L. Samples with higher D-sorbitol concentrations were estimated after dilution. The method was used to measure disposition curves of sorbitol in volunteers after a single intravenous dose of 0.8 g sorbitol.     

用于高压电容器的SrTiO3基陶瓷

报道ＳｒＴｉＯ３基陶瓷的制备方法和介电性质，给出了用此种陶瓷制成的高压电容器的试验结果，并对它位进行了讨论。     

Mass customization of medical devices and implants: state of the art and future directions

The medical field is one in which the need for customization can be clear cut, as providing tailored devices and implants for unique physiologies can provide for a better overall treatment than the use of 'off the shelf' devices and implants. Customization in the production of medical products can be roughly divided into consideration of medical devices, and of implantable parts or systems. The present paper outlines the current state of the art in both of these areas, presents details of projects that are ongoing at the University of Leeds and outlines future research directions.     

吏用Authorware开发CAI多媒体课件的几个关键技术

本文以CAI课件的开发制作过程遇到的一些关键的技术问题加以探讨．并结合实际制作介绍了一些技艺方法、     

精彩展品

环保铁皮船 这艘铁皮船的动力由一支短短的普通蜡烛提供，不但前进、转弯等自如，而且支持时间可长达数小时，令不少买家非常感兴趣。专做德国生意的熊猫贸易老板陈祥龙认为，它既造价低廉又符合环保概念，在国外应该很有市场。凭他经验估计，以8元批发价拿到的产品，售价大约可以卖到12元，利润可观。     

欧盟邻苯二甲酸盐新规明年实施

欧盟最新颁布的关于邻苯二甲酸盐的新指导标准（第2005／84／EC），将于2007年1月16日起开始执行。     

Nutrient removal performance of an anaerobic–anoxic–aerobic process as a function of influent C/P ratio

The laboratory scale anaerobic–anoxic–aerobic (A²O) process fed with synthetic brewage wastewater was designed to investigate the effects of changing feed C/P ratio on the performance of biological nutrient removal (BNR) processes. In the experiment, the influent chemical oxygen demand (COD) concentration was kept at approximately 300 mg L^?1 while the total phosphorus concentration was varied to obtain the desired C/P ratio. Results showed that when the C/P ratio was lower than 32, phosphorus removal efficiency increased as C/P ratio increased linearly, while when the C/P ratio was higher than 32, the P removal efficiency was maintained at 90–98%, and effluent P concentration was lower than 0.5 mg L^?1. However, regardless of the C/P ratio, excellent COD removal (90% or higher) and good total nitrogen removal (75–84%) were maintained throughout the experiments. It was also found that very good linear correlation was obtained between COD uptake per unit P released in the anaerobic zone and C/P ratio. In addition, the P content in the wasted activated sludge increased with the decrease in the C/P ratio. Based on the results, it was recommended that the wastewater C/P ratio and its effects be incorporated into BNR design and operational procedures, appropriate C/P ratios were used to achieve the effluent treatment goals. Copyright © 2005 Society of Chemical Industry     

知识驱动的UG中的产品开发

重点论述了UG中的KBE即KF模块的知识驱动产品开发设计过程及其关键技术，介绍了知识驱动自动化过程，实例剖析了UG中的KF在产品开发设计过程中的应用。     

日本年轻建筑师的现状——1960年代的这一群

一人脉对照 1 日本现代建筑师溯流 引领日本现代建筑进人成熟时期的领袖人物非丹下健三莫属。由师从丹下的模文彦（1928年-）、黑川纪章（1934年-）以及大高正人（1923年-）、菊竹清训（1928年-）等4人在1960年代创导的新陈代谢运动，使主流的欧美建筑界开始认真关注一个亚洲的小国——日本的建筑活动。可以说新陈代谢运动是日本现代建筑发展的基石也是随后其建筑发展的原动力。