Bufalin Inhibits Tumorigenesis,Stemness, and Epithelial–Mesenchymal Transition in Colorectal Cancer through a C-Kit/Slug Signaling Axis

Colorectal cancer (CRC) is a major source of morbidity and mortality, characterized by intratumoral heterogeneity and the presence of cancer stem cells (CSCs). Bufalin has potent activity against many tumors, but studies of its effect on CRC stemness are limited. We explored bufalin’s function and mechanism using CRC patient-derived organoids (PDOs) and cell lines. In CRC cells, bufalin prevented nuclear translocation of β-catenin and down-regulated CSC markers (CD44, CD133, LGR5), pluripotency factors, and epithelial–mesenchymal transition (EMT) markers (N-Cadherin, Slug, ZEB1). Functionally, bufalin inhibited CRC spheroid formation, aldehyde dehydrogenase activity, migration, and invasion. Network analysis identified a C-Kit/Slug signaling axis accounting for bufalin’s anti-stemness activity. Bufalin treatment significantly downregulated C-Kit, as predicted. Furthermore, overexpression of C-Kit induced Slug expression, spheroid formation, and bufalin resistance. Similarly, overexpression of Slug resulted in increased expression of C-Kit and identical functional effects, demonstrating a pro-stemness feedback loop. For further study, we established PDOs from diagnostic colonoscopy. Bufalin differentially inhibited PDO growth and proliferation, induced apoptosis, restored E-cadherin, and downregulated CSC markers CD133 and C-Myc, dependent on C-Kit/Slug. These findings suggest that the C-Kit/Slug axis plays a pivotal role in regulating CRC stemness, and reveal that targeting this axis can inhibit CRC growth and progression.     

SecMVX:Analysis on the Vulnerability of Multi-Variant Execution

As an active defenses technique, multi-variant execution(MVX) can detect attacks by moni-toring the consistency of heterogeneous variants with parallel executio...     

复杂过程分析的模糊聚类分类器设计及其性能

本文采用模式识别方法推断烧结矿质量。在给出模糊系统聚类分析算法基础上,用软件实现了基于模糊聚类分类器和动态聚类分类器,并用现场实测的样本采用\     

土壤腐蚀性关键因素的评价与选取

在22种土壤腐蚀性因素的聚类分析的基础上,通过对单个因素分类能力的特征评价,从同类中选取关键性因素,进而在特征空间中进行有关样本的土壤腐蚀性预测和考评,为今后土壤腐蚀性研究中主要因素的选取和等级评判提供一定的依据。     

材料大气腐蚀数据库应用软件的研制

从材料大气腐蚀数据库应用系统如何在IBM/PC-XT计算机上实现的角度,介绍它的机器环境和系统构成,详细地叙述了该系统的应用程序。该系统的优点是操作方便,容易掌握。它的应用对实现全国大气腐蚀试验站的管理科学化具有重要意义。     

Consequence calculation for relocation after nuclear accidents

This paper presents the results of dose and cost calculation for relocation after nuclear accidents. In order to quantify the relationship between radiation dose and relevant parameters defining protective actions, well-designed calculations have been performed and the results analyzed. On the basis of this, some dependencies between the important parameters describing the features of relocation and decontamination have been mathematically formulated. The similar has also been done for some economic costs as a result of implementing the countermeasures considered in this paper.     

LED环氧树脂封装材料研究进展

针对封装材料在使用中的缺陷,综述了对环氧树脂的增韧、提高耐热性、改善透明性、改善加工性能的研究现状,并介绍了LED环氧树脂封装材料的发展前景。     

MIDP2.0中AMS对MIDlet生命周期控制的设计与实现

本文首先对MIDP2．0和AMS进行了简要介绍。然后对MIDlet的生命周期展开了详细的讨论。最后通过对几个协同完成控制功能的类的分析,描述了AMS对MIDlet生命周期控制的设计和实现。     

动态注射成型机塑化过程中的分散效应

采用电磁动态注射成型机塑化聚丙烯(PP)与纳米CaCO_3的共混料。通过扫描电子显微镜观察以及差示扫描量热法分析,探讨了振动参数对注射机预塑过程中分散效应的影响。实验发现,随着振动频率和振幅的增加,纳米CaCO_3在PP熔体中分散得更均匀,平均粒径逐渐减小;混合料的熔点向高温移动,混合质量得以改善,有利于完善结晶。因而表明,引入振动力场有效地促进了CaCO_3的分散,提高了注塑机的塑化效果。