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Binding of zinc to bovine and human milk proteins 总被引:1,自引:0,他引:1
Zn binding by whole bovine and human casein and by purified bovine caseins and whey proteins was investigated by equilibrium dialysis. Bovine alpha s1-casein had the greatest Zn-binding capacity (approximately 11 atoms Zn/mol). Protein aggregation was observed as Zn concentration was increased and the protein precipitated at a free Zn concentration of 1.7 mM. Zn binding increased with increasing pH in the range 5.4-7.0 and decreased with increasing ionic strength. Competition between Zn and Ca was observed for binding to alpha s1-casein indicating common binding sites for these two metals. Bovine beta-casein bound up to 8 atoms Zn/mol and precipitated at a free Zn concentration of approximately 2.5 mM, while kappa-casein bound 1-2 atoms Zn/mol. Whole bovine and human casein bound 5-8 atoms Zn/mol and precipitated at a free Zn concentration of approximately 2.0 mM. Scatchard plots for Zn binding to caseins showed upward convexity, possibly due to Zn-induced association of caseins. Apparent average association constants (Kapp) for all caseins were similar (log Kapp 3.0-3.2). Enzymic dephosphorylation of alpha s1- or whole bovine casein markedly reduced, but did not eliminate, Zn binding. Thus, phosphoserine residues appeared to be the primary Zn-binding sites in caseins. With the exception of bovine serum albumin, which bound over 8 atoms Zn/mol, the bovine whey proteins, beta-lactoglobulin, alpha-lactalbumin and lactotransferrin, had little capacity for Zn binding. 相似文献
44.
OBJECTIVE: To propose a definition for stage IA1 cervical adenocarcinoma, based on the International Federation of Gynecology and Obstetrics (FIGO) staging system, and to determine if patients meeting criteria might be candidates for conservative surgery. METHODS: Two hundred women were diagnosed with early-stage cervical adenocarcinoma from 1982 to 1996. Histopathologic sections were reviewed by a gynecologic pathologist. Medical records were reviewed, and patients included in this study had microscopically identifiable lesions, up to 3 mm invasive depth, up to 7 mm tumor width, and negative margins if cone biopsy was performed. RESULTS: Twenty-one patients with microinvasive adenocarcinoma met criteria for FIGO stage IA1 carcinoma of the cervix. The median (range) follow-up was 76 (30-172) months and median (range) patient age was 38 (24-75) years. Definitive treatment included type II or III radical hysterectomy in 16 cases, simple abdominal or vaginal hysterectomy in four cases, and loop electrosurgical excision procedure in one case; one patient received adjuvant pelvic radiation. The histologic subtypes were endocervical adenocarcinoma in 18 cases, adenosquamous carcinoma in two cases, and clear-cell adenocarcinoma in one case. There was no evidence of parametrial invasion or lymph node metastases in any patient who had radical surgery, and there were no disease recurrences. CONCLUSION: Patients with microinvasive adenocarcinoma who met criteria for FIGO stage IA1 cervical carcinoma had disease limited to the cervix, and conservative surgery, such as cone biopsy or simple hysterectomy, might offer them definitive treatment. 相似文献
45.
Watta and Hassoun (1996) proposed a coupled gradient neural network for mixed integer programming. In this network continuous neurons were used to represent discrete variables. For the larger temporal problem they attempted many of the solutions found were infeasible. This paper proposes an augmented Hopfield network which is similar to the coupled gradient network proposed by Watta and Hassoun. However, in this network truly discrete neurons are used. It is shown that this network can be applied to mixed integer programming. Results illustrate that feasible solutions are now obtained for the larger temporal problem. 相似文献
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This case of ectopic pancreas found in the pre-pyloric channel of a 2-day-old infant is unique. A review of the literature reveals no other cases of symptomatic ectopic pancreas in an infant of this age. In this patient, signs and symptoms were consistent with pyloric stenosis. Upper gastrointestinal study and esophagogastroduodenoscopy (EGD) revealed the diagnosis. This case is examined and the literature is reviewed. 相似文献
48.
DA McCubbrey DD Cody EL Peterson JL Kuhn MJ Flynn SA Goldstein 《Canadian Metallurgical Quarterly》1995,28(8):891-899
This study investigated (1) whether a characterization of the macroscopic architecture within the vertebral centrum would improve predictions of vertebral strength, (2) if regions in the centrum where least bone loss with age occurs are more predictive of vertebral strength, and (3) whether different patterns of the macroscopic architecture are predictive of static as compared to fatigue strength. To characterize the vertebral macroscopic architecture, a regional bone mineral density (rBMD) technique was used that estimated the cancellous density distribution (in 18 specific regions of the vertebral centrum) for vertebrae T7-L4, from spines of 20 female cadavers. Static and fatigue failure properties of whole vertebrae were obtained, and predictive models of static and fatigue failure properties of whole vertebrae were examined. We found that (1) vertebral failure properties were better predicted by combinations of vertebral regional cancellous density (multiple linear regressions) rather than by any individual region of cancellous density alone (simple linear regressions); (2) models using regions of density that demonstrated minimum decline with age [from the data of Flynn and Cody (Calcif. Tissue Int. 53, S170-S175 (1993))] resulted in better correlations with ex vivo vertebral static failure properties than models using density regions that showed maximum decline with age, and (3) static and fatigue characteristics required different density regions to reach significance. (A comparison of models predictive of static and fatigue failure properties revealed that anterior density regions were most often included in predictive models of the static properties while posterior regions were more predictive of the fatigue properties).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
49.
This paper presents an integrative model developed this past year by the Building Construction (BC) Department at Virginia Tech (VT), designed to help each student master the competencies necessary to succeed in the 21st Century as a building constructor in a changing global market-place. The curriculum described in this paper presents a dynamic, practical, applied academic model; balances the construction education concepts of practical experience based knowledge with academic inquiry; integrates people and communication skills with the pragmatic building construction skills; and provides a construction program that maintains a strong identity positioned between architecture and engineering. Strategically this task was accomplished by weaving vertical and horizontal integration into the BC curriculum at Virginia Tech. Results of this first year are presented, including lessons learned, external evaluations, and recommendations for adaptation. 相似文献
50.
The present research examines episodes of favor exchange among peer employees. We posit that favor receivers' and favor givers' commitment to their exchange relationships with one another will be accounted for by different factors. As predicted, in 2 different organizational contexts, receivers' commitment to their relationships with givers was found to be more related to their judgments of the givers' interactional justice when performing the favor, whereas givers' commitment to their relationships with receivers was shown to be more associated with their judgments of the favorability of the outcomes associated with the favor that they performed. The implications of these findings for how givers and receivers can better manage favor exchange, and hence their relationships with each other, are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献